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A noncomplementation screen for quantitative trait alleles in saccharomyces cerevisiae

Authors
 Hyun Seok Kim  ;  Juyoung Huh  ;  Linda Riles  ;  Alejandro Reyes  ;  Justin C. Fay 
Citation
 G3-GENES GENOMES GENETICS, Vol.2(7) : 753-760, 2012 
Journal Title
 G3-GENES GENOMES GENETICS 
Issue Date
2012
MeSH
Alleles ; Catalase/genetics ; Cystathionine beta-Synthase/genetics ; Genetic Complementation Test ; Hemizygote ; Mutation ; Phenotype ; Quantitative Trait Loci* ; Saccharomyces cerevisiae/genetics* ; Saccharomyces cerevisiae Proteins/genetics
Keywords
complex trait ; copper sulfate ; hydrogen sulfide
Abstract
Both linkage and linkage disequilibrium mapping provide well-defined approaches to mapping quantitative trait alleles. However, alleles of small effect are particularly difficult to refine to individual genes and causative mutations. Quantitative noncomplementation provides a means of directly testing individual genes for quantitative trait alleles in a fixed genetic background. Here, we implement a genome-wide noncomplementation screen for quantitative trait alleles that affect colony color or size by using the yeast deletion collection. As proof of principle, we find a previously known allele of CYS4 that affects colony color and a novel allele of CTT1 that affects resistance to hydrogen peroxide. To screen nearly 4700 genes in nine diverse yeast strains, we developed a high-throughput robotic plating assay to quantify colony color and size. Although we found hundreds of candidate alleles, reciprocal hemizygosity analysis of a select subset revealed that many of the candidates were false positives, in part the result of background-dependent haploinsufficiency or second-site mutations within the yeast deletion collection. Our results highlight the difficulty of identifying small-effect alleles but support the use of noncomplementation as a rapid means of identifying quantitative trait alleles of large effect.
Files in This Item:
T201206441.pdf Download
DOI
10.1534/g3.112.002550
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
Kim, Hyun Seok(김현석) ORCID logo https://orcid.org/0000-0003-4498-8690
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/158366
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