78 98

Cited 61 times in

Uropod elongation is a common final step in leukocyte extravasation through inflamed vessels

 Young-Min Hyun  ;  Ronen Sumagin  ;  Pranita P. Sarangi  ;  Elena Lomakina  ;  Michael G. Overstreet  ;  Christina M. Baker  ;  Deborah J. Fowell  ;  Richard E. Waugh  ;  Ingrid H. Sarelius  ;  Minsoo Kim 
 JOURNAL OF EXPERIMENTAL MEDICINE, Vol.209(7) : 1349-1362, 2012 
Journal Title
Issue Date
Animals ; CD18 Antigens/genetics ; CD18 Antigens/metabolism ; Cell Adhesion/genetics ; Cell Adhesion/physiology ; Cell Surface Extensions/genetics ; Cell Surface Extensions/physiology* ; Cells, Cultured ; Endothelium, Vascular/metabolism ; Endothelium, Vascular/pathology ; Human Umbilical Vein Endothelial Cells/metabolism ; Humans ; Integrin alpha3beta1/deficiency ; Integrin alpha3beta1/genetics ; Leukocytes/metabolism ; Leukocytes/physiology* ; Leukocytes/ultrastructure ; Luminescent Proteins/genetics ; Luminescent Proteins/metabolism ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Mice, Transgenic ; Microscopy, Electron ; Microscopy, Fluorescence/methods ; Neutrophils/metabolism ; Neutrophils/physiology ; Neutrophils/ultrastructure ; T-Lymphocytes/metabolism ; T-Lymphocytes/physiology ; T-Lymphocytes/ultrastructure ; Transendothelial and Transepithelial Migration/genetics ; Transendothelial and Transepithelial Migration/physiology* ; Vasculitis/genetics ; Vasculitis/metabolism*
The efficient trafficking of immune cells into peripheral nonlymphoid tissues is key to enact their protective functions. Despite considerable advances in our understanding of cell migration in secondary lymphoid organs, real-time leukocyte recruitment into inflamed tissues is not well characterized. The conventional multistep paradigm of leukocyte extravasation depends on CD18 integrin-mediated events such as rapid arrest and crawling on the surface of the endothelium and transmigration through the endothelial layer. Using enhanced three-dimensional detection of fluorescent CD18 fusion proteins in a newly developed knockin mouse, we report that extravasating leukocytes (neutrophils, monocytes, and T cells) show delayed uropod detachment and become extremely elongated before complete transmigration across the endothelium. Additionally, these cells deposit CD18(+) microparticles at the subendothelial layer before retracting the stretched uropod. Experiments with knockout mice and blocking antibodies reveal that the uropod elongation and microparticle formation are the result of LFA-1-mediated adhesion and VLA-3-mediated cell migration through the vascular basement membrane. These findings suggest that uropod elongation is a final step in the leukocyte extravasation cascade, which may be important for precise regulation of leukocyte recruitment into inflamed tissues.
Files in This Item:
T201206392.pdf Download
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
Yonsei Authors
Hyun, Young-Min(현영민) ORCID logo https://orcid.org/0000-0002-0567-2039
사서에게 알리기


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.