Cited 11 times in
Snail, a transcriptional regulator, represses adiponectin expression by directly binding to an E-box motif in the promoter
DC Field | Value | Language |
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dc.contributor.author | 김경섭 | - |
dc.contributor.author | 이용호 | - |
dc.contributor.author | 이현철 | - |
dc.date.accessioned | 2018-05-10T06:36:41Z | - |
dc.date.available | 2018-05-10T06:36:41Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 0026-0495 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/158301 | - |
dc.description.abstract | Objective : Adiponectin is a hormone that modulates many metabolic processes and is exclusively expressed in adipose tissue. However, complete understanding of the factors that regulate adiponectin expression is lacking. The following were investigated: (1) functional analysis of the human adiponectin promoter, (2) putative adiponectin repressor sequence activity in 3T3-L1 adipocytes using promoter mutagenesis, (3) whether Snail, an E-box binding transcription factor, binds this repressor sequence, (4) if Snail regulates adiponectin expression in 3T3-L1 pre-adipocytes. Materials/Methods: To further understand how adiponectin expression is regulated, we isolated the human adiponectin promoter and analyzed its activity after serial deletions. Results : We found a negative cis-regulatory element located in the adiponectin proximal promoter sequence (-174 to -152 bp), which contained an E-box site (CAACTG). The DNA binding activity of this putative negative regulatory factor was found to be sequence-specific and the binding activity is decreased during adipocyte differentiation time-dependently. Affinity chromatography identified the zinc-finger transcription factor Snail (SNAI1) as the putative negative regulatory factor. Chromatin immunoprecipitation assay and electrophoretic mobility shift assay confirmed that Snail binds to this negative cis-regulatory element in pre-adipocytes, exclusively. Inhibition of Snail expression using small interfering RNA techniques increased adiponectin expression in 3T3-L1 adipocytes, while overexpression of Snail reduced adiponectin expression. Furthermore, we observed an inverse relation between the expression of Snail and the expression of CCAAT-enhancer-binding protein alpha and peroxisome proliferator-activated receptor gamma, which are transcription factors that regulate adipogenesis. Conclusions : Snail is a novel regulator of adiponectin expression and probably has a role in regulating adipogenesis. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | W.B. Saunders | - |
dc.relation.isPartOf | METABOLISM-CLINICAL AND EXPERIMENTAL | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | 3T3-L1 Cells | - |
dc.subject.MESH | Adiponectin/genetics* | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Base Sequence | - |
dc.subject.MESH | Binding Sites | - |
dc.subject.MESH | Blotting, Western | - |
dc.subject.MESH | Chromatin Immunoprecipitation | - |
dc.subject.MESH | DNA Primers | - |
dc.subject.MESH | Electrophoretic Mobility Shift Assay | - |
dc.subject.MESH | Gene Silencing | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Polymerase Chain Reaction | - |
dc.subject.MESH | Promoter Regions, Genetic* | - |
dc.subject.MESH | Snail Family Transcription Factors | - |
dc.subject.MESH | Transcription Factors/genetics | - |
dc.subject.MESH | Transcription Factors/physiology* | - |
dc.title | Snail, a transcriptional regulator, represses adiponectin expression by directly binding to an E-box motif in the promoter | - |
dc.type | Article | - |
dc.contributor.college | 의과대학 내과학교실 | - |
dc.contributor.department | 내과학교실 | - |
dc.contributor.googleauthor | Young Mi Park | - |
dc.contributor.googleauthor | Yong-ho Lee | - |
dc.contributor.googleauthor | Soo Hyun Kim | - |
dc.contributor.googleauthor | Eun Young Lee | - |
dc.contributor.googleauthor | Kyung-Sup Kim | - |
dc.contributor.googleauthor | Darren R. Williams | - |
dc.contributor.googleauthor | Hyun Chul Lee | - |
dc.identifier.doi | 10.1016/j.metabol.2012.04.014 | - |
dc.contributor.localId | A02989 | - |
dc.contributor.localId | A03301 | - |
dc.relation.journalcode | J02223 | - |
dc.identifier.eissn | 1532-8600 | - |
dc.identifier.pmid | 22595290 | - |
dc.identifier.url | http://www.metabolismjournal.com/article/S0026-0495(12)00172-2/abstract | - |
dc.contributor.alternativeName | Lee, Yong Ho | - |
dc.contributor.alternativeName | Lee, Hyun Chul | - |
dc.contributor.affiliatedAuthor | Lee, Yong Ho | - |
dc.contributor.affiliatedAuthor | Lee, Hyun Chul | - |
dc.citation.volume | 61 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 1622 | - |
dc.citation.endPage | 1632 | - |
dc.identifier.bibliographicCitation | METABOLISM-CLINICAL AND EXPERIMENTAL, Vol.61(11) : 1622-1632, 2012 | - |
dc.identifier.rimsid | 40676 | - |
dc.type.rims | ART | - |
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