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CD11b+ Gr1+ bone marrow cells ameliorate liver fibrosis by producing interleukin-10 in mice

DC FieldValueLanguage
dc.contributor.author김자경-
dc.contributor.author서양권-
dc.contributor.author이관식-
dc.contributor.author한광협-
dc.date.accessioned2018-05-10T06:36:02Z-
dc.date.available2018-05-10T06:36:02Z-
dc.date.issued2012-
dc.identifier.issn0270-9139-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/158277-
dc.description.abstractClinical trials and animal models suggest that infusion of bone marrow cells (BMCs) is effective therapy for liver fibrosis, but the underlying mechanisms are obscure, especially those associated with early effects of BMCs. Here, we analyzed the early impact of BMC infusion and identified the subsets of BMCs showing antifibrotic effects in mice with carbon tetrachloride-induced liver fibrosis. An interaction between BMCs and activated hepatic stellate cells (HSCs) was investigated using an in vitro coculturing system. Within 24 hours, infused BMCs were in close contact with activated HSCs, which was associated with reduced liver fibrosis, enhanced hepatic expression of interleukin (IL)-10, and expanded regulatory T cells but decreased macrophage infiltration in the liver at 24 hours after BMC infusion. In contrast, IL-10-deficient (IL-10(-/-) ) BMCs failed to reproduce these effects in fibrotic livers. Intriguingly, in isolated cells, CD11b(+) Gr1(high) F4/80(-) and CD11b(+) Gr1(+) F4/80(+) BMCs expressed more IL-10 after coculturing with activated HSCs, leading to suppressed expression of collagen and α-smooth muscle actin in HSCs. Moreover, these effects were either enhanced or abrogated, respectively, when BMCs were cocultured with IL-6(-/-) and retinaldehyde dehydrogenase 1(-/-) HSCs. Similar to murine data, human BMCs expressed more IL-10 after coculturing with human HSC lines (LX-2 or hTERT), and serum IL-10 levels were significantly elevated in patients with liver cirrhosis after autologous BMC infusion. Conclusion : Activated HSCs increase IL-10 expression in BMCs (CD11b(+) Gr1(high) F4/80(-) and CD11b(+) Gr1(+) F4/80(+) cells), which in turn ameliorates liver fibrosis. Our findings could enhance the design of BMC therapy for liver fibrosis.-
dc.description.statementOfResponsibilityopen-
dc.formatapplication/pdf-
dc.languageEnglish-
dc.publisherWiley-
dc.relation.isPartOfHEPATOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHActins/metabolism-
dc.subject.MESHAnimals-
dc.subject.MESHBone Marrow Cells/immunology-
dc.subject.MESHBone Marrow Cells/metabolism*-
dc.subject.MESHBone Marrow Transplantation-
dc.subject.MESHCD11b Antigen/metabolism-
dc.subject.MESHCD4 Antigens/metabolism-
dc.subject.MESHCarbon Tetrachloride-
dc.subject.MESHCell Communication-
dc.subject.MESHChemokine CCL2/genetics-
dc.subject.MESHChemokine CCL2/metabolism-
dc.subject.MESHCollagen Type I/metabolism-
dc.subject.MESHForkhead Transcription Factors/metabolism-
dc.subject.MESHHepatic Stellate Cells/immunology-
dc.subject.MESHHepatic Stellate Cells/metabolism*-
dc.subject.MESHHumans-
dc.subject.MESHInterleukin-1/genetics-
dc.subject.MESHInterleukin-1/metabolism-
dc.subject.MESHInterleukin-10/immunology-
dc.subject.MESHInterleukin-10/metabolism*-
dc.subject.MESHInterleukin-2 Receptor alpha Subunit/metabolism-
dc.subject.MESHLiver Cirrhosis/chemically induced-
dc.subject.MESHLiver Cirrhosis/immunology*-
dc.subject.MESHLiver Cirrhosis/metabolism*-
dc.subject.MESHLiver Cirrhosis/pathology-
dc.subject.MESHMale-
dc.subject.MESHMice-
dc.subject.MESHMice, Inbred C57BL-
dc.subject.MESHMice, Nude-
dc.subject.MESHMice, Transgenic-
dc.subject.MESHRNA, Messenger/metabolism-
dc.subject.MESHReceptors, Chemokine/metabolism-
dc.subject.MESHStatistics, Nonparametric-
dc.subject.MESHT-Lymphocytes, Regulatory/immunology*-
dc.subject.MESHT-Lymphocytes, Regulatory/metabolism-
dc.subject.MESHTransforming Growth Factor beta1/genetics-
dc.subject.MESHTransforming Growth Factor beta1/metabolism-
dc.titleCD11b+ Gr1+ bone marrow cells ameliorate liver fibrosis by producing interleukin-10 in mice-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Internal Medicine-
dc.contributor.googleauthorYang-Gun Suh-
dc.contributor.googleauthorJa Kyung Kim-
dc.contributor.googleauthorJin-Seok Byun-
dc.contributor.googleauthorHyon-Seung Yi-
dc.contributor.googleauthorYoung-Sun Lee-
dc.contributor.googleauthorHyuk Soo Eun-
dc.contributor.googleauthorSo Yeon Kim-
dc.contributor.googleauthorKwang-Hyub Han-
dc.contributor.googleauthorKwan Sik Lee-
dc.contributor.googleauthorGregg Duester-
dc.contributor.googleauthorScott L. Friedman-
dc.contributor.googleauthorWon-Il Jeong-
dc.identifier.doi10.1002/hep.25817-
dc.contributor.localIdA00852-
dc.contributor.localIdA01891-
dc.contributor.localIdA02666-
dc.contributor.localIdA04268-
dc.relation.journalcodeJ00985-
dc.identifier.eissn1527-3350-
dc.identifier.pmid22544759-
dc.contributor.alternativeNameKim, Ja Kyung-
dc.contributor.alternativeNameSuh, Yang Gun-
dc.contributor.alternativeNameLee, Kwan Sik-
dc.contributor.alternativeNameHan, Kwang Hyup-
dc.contributor.affiliatedAuthorKim, Ja Kyung-
dc.contributor.affiliatedAuthorSuh, Yang Gun-
dc.contributor.affiliatedAuthorLee, Kwan Sik-
dc.contributor.affiliatedAuthorHan, Kwang Hyup-
dc.citation.volume56-
dc.citation.number5-
dc.citation.startPage1902-
dc.citation.endPage1912-
dc.identifier.bibliographicCitationHEPATOLOGY, Vol.56(5) : 1902-1912, 2012-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학교실) > 1. Journal Papers

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