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Production and characterization of beta-1,4-glucosidase by a strain of Penicillium pinophilum

Authors
 Ah-Reum Joo  ;  Marimuthu Jeya  ;  Kyoung-Mi Lee  ;  Kyoung-Min Lee  ;  Hee-Jung Moon  ;  Yeong-Suk Kim  ;  Jung-Kul Lee 
Citation
 PROCESS BIOCHEMISTRY, Vol.45(6) : 851-858, 2010 
Journal Title
PROCESS BIOCHEMISTRY
ISSN
 1359-5113 
Issue Date
2010
Keywords
Enzyme production ; β-Glucosidase ; Glycoside hydrolase family 3 ; Penicillium pinophilum
Abstract
A high β-glucosidase (BGL)-producing strain was isolated and identified as Penicillium pinophilum KMJ601 based on its morphology and internal transcribed spacer rDNA gene sequence. Under the optimal culture conditions, a maximum BGL specific activity of 3.2 U ml−1 (83 U mg-protein−1), one of the highest levels among BGL-producing microorganisms was obtained. An extracellular BGL was purified to homogeneity by sequential chromatography of P. pinophilum culture supernatants on a DEAE-Sepharose column, a gel filtration column, and then on a Mono Q column. The relative molecular weight of P. pinophilum BGL was determined to be 120 kDa by SDS-PAGE and size exclusion chromatography, indicating that the enzyme is a monomer. The hydrolytic activity of the BGL had a pH optimum of 3.5 and a temperature optimum of 32 °C. P. pinophilum BGL showed a higher activity (Vmax = 1120 U mg-protein−1) than most BGLs purified from other sources. The internal amino acid sequences of P. pinophilum BGL showed a significant homology with hydrolases from glycoside hydrolase family 3. Although BGLs have been purified and characterized from several other sources, P. pinophilum BGL is distinguished from other BGLs by its high activity.
Full Text
https://www.sciencedirect.com/science/article/pii/S1359511310000437
DOI
10.1016/j.procbio.2010.02.005
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
Lee, Kyoung Mi(이경미) ORCID logo https://orcid.org/0000-0002-9038-8162
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/158130
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