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Visualization of integrin Mac-1 in vivo

DC FieldValueLanguage
dc.contributor.author현영민-
dc.date.accessioned2018-03-26T17:11:08Z-
dc.date.available2018-03-26T17:11:08Z-
dc.date.issued2015-
dc.identifier.issn0022-1759-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/157304-
dc.description.abstractβ2 integrins play critical roles in migration of immune cells and in the interaction with other cells, pathogens, and the extracellular matrix. Among the β2 integrins, Mac-1 (Macrophage antigen-1), composed of CD11b and CD18, is mainly expressed in innate immune cells and plays a major role in cell migration and trafficking. In order to image Mac-1-expressing cells both in live cells and mouse, we generated a knock-in (KI) mouse strain expressing CD11b conjugated with monomeric yellow fluorescent protein (mYFP). Expression of CD11b-mYFP protein was confirmed by Western blot and silver staining of CD11b-immunoprecipitates and total cell lysates from the mouse splenocytes. Mac-1-mediated functions of the KI neutrophils were comparable with those in WT cells. The fluorescence intensity of CD11b-mYFP was sufficient to image CD11b expressing cells in live mice using intravital two-photon microscopy. In vitro, dynamic changes in the intracellular localization of CD11b molecules could be measured by epifluorescent microscopy. Finally, CD11b-expressing immune cells from tissue were easily detected by flow cytometry without anti-CD11b antibody staining.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherElsevier-
dc.relation.isPartOfJOURNAL OF IMMUNOLOGICAL METHODS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHBacterial Proteins/genetics-
dc.subject.MESHCD11b Antigen/immunology*-
dc.subject.MESHCD18 Antigens/immunology-
dc.subject.MESHCell Adhesion-
dc.subject.MESHCell Movement/immunology-
dc.subject.MESHExtracellular Matrix/immunology-
dc.subject.MESHFlow Cytometry-
dc.subject.MESHGene Knock-In Techniques-
dc.subject.MESHIntercellular Adhesion Molecule-1/metabolism-
dc.subject.MESHLuminescent Proteins/genetics-
dc.subject.MESHMacrophage-1 Antigen/analysis*-
dc.subject.MESHMacrophage-1 Antigen/immunology*-
dc.subject.MESHMice-
dc.subject.MESHMice, Inbred C57BL-
dc.subject.MESHMice, Transgenic-
dc.subject.MESHMicroscopy, Fluorescence-
dc.subject.MESHNeutrophils/immunology*-
dc.subject.MESHPhagocytosis/immunology-
dc.subject.MESHSpleen/cytology*-
dc.subject.MESHSpleen/immunology-
dc.subject.MESHStaining and Labeling/methods-
dc.titleVisualization of integrin Mac-1 in vivo-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Anatomy-
dc.contributor.googleauthorKihong Lim-
dc.contributor.googleauthorYoung-Min Hyun-
dc.contributor.googleauthorKris Lambert-Emo-
dc.contributor.googleauthorDavid J Topham-
dc.contributor.googleauthorMinsoo Kim-
dc.identifier.doi10.1016/j.jim.2015.08.012-
dc.contributor.localIdA04814-
dc.relation.journalcodeJ01449-
dc.identifier.eissn1872-7905-
dc.identifier.pmid26342259-
dc.subject.keywordCD11b-
dc.subject.keywordImaging-
dc.subject.keywordIntegrin-
dc.subject.keywordMAC-1-
dc.subject.keywordYFP-
dc.contributor.alternativeNameHyun, Young-Min-
dc.contributor.affiliatedAuthorHyun, Young-Min-
dc.citation.volume426-
dc.citation.number--
dc.citation.startPage120-
dc.citation.endPage127-
dc.identifier.bibliographicCitationJOURNAL OF IMMUNOLOGICAL METHODS, Vol.426(-) : 120-127, 2015-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers

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