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Hypoxia increases epithelial permeability in human nasal epithelia

DC Field Value Language
dc.contributor.author김창훈-
dc.contributor.author윤주헌-
dc.date.accessioned2018-03-26T17:09:34Z-
dc.date.available2018-03-26T17:09:34Z-
dc.date.issued2015-
dc.identifier.issn0513-5796-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/157255-
dc.description.abstractPURPOSE: The nasal mucosa is the first site to encounter pathogens, and it forms continuous barriers to various stimuli. This barrier function is very important in the innate defense mechanism. Additionally, inflammation of the nasal sinus is known to be a hypoxic condition. Here, we studied the effect of hypoxia on barrier function in normal human nasal epithelial (NHNE) cells. MATERIALS AND METHODS: The expression levels of various junction complex proteins were assessed in hypoxia-stimulated NHNE cells and human nasal mucosal tissues. We performed real-time polymerase chain reaction analysis, western blotting, and immunofluorescence assays to examine differences in the mRNA and protein expression of ZO-1, a tight junction protein, and E-cadherin in NHNE cells. Moreover, we evaluated the trans-epithelial resistance (TER) of NHNE cells under hypoxic conditions to check for changes in permeability. The expression of ZO-1 and E-cadherin was measured in human nasal mucosa samples by western blotting. RESULTS: Hypoxia time-dependently decreased the expression of ZO-1 and E-cadherin at the gene and protein levels. In addition, hypoxia decreased the TER of NHNE cells, which indicates increased permeability. Human nasal mucosa samples, which are supposed to be hypoxic, showed significantly decreased levels of ZO-1 and E-cadherin expression compared with control. CONCLUSION: Our results demonstrate that hypoxia altered the expression of junction complex molecules and increased epithelial permeability in human nasal epithelia. This suggests that hypoxia causes barrier dysfunction. Furthermore, it may be associated with innate immune dysfunction after encountering pathogens.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherYonsei University-
dc.relation.isPartOfYONSEI MEDICAL JOURNAL-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHBlotting, Western-
dc.subject.MESHCadherins/analysis*-
dc.subject.MESHCadherins/genetics-
dc.subject.MESHEpithelium/chemistry-
dc.subject.MESHEpithelium/pathology-
dc.subject.MESHHumans-
dc.subject.MESHHypoxia/etiology-
dc.subject.MESHHypoxia/metabolism*-
dc.subject.MESHMembrane Proteins/analysis*-
dc.subject.MESHNasal Mucosa/chemistry*-
dc.subject.MESHNasal Mucosa/pathology-
dc.subject.MESHNasal Mucosa/secretion*-
dc.subject.MESHPermeability/radiation effects*-
dc.subject.MESHRNA, Messenger/genetics-
dc.subject.MESHRNA, Messenger/metabolism-
dc.subject.MESHReal-Time Polymerase Chain Reaction-
dc.subject.MESHTight Junctions/metabolism*-
dc.subject.MESHZonula Occludens-1 Protein-
dc.titleHypoxia increases epithelial permeability in human nasal epithelia-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Otorhinolaryngology-
dc.contributor.googleauthorHyun Jin Min-
dc.contributor.googleauthorTae Hoon Kim-
dc.contributor.googleauthorJoo-Heon Yoon-
dc.contributor.googleauthorChang-Hoon Kim-
dc.identifier.doi10.3349/ymj.2015.56.3.825-
dc.contributor.localIdA01050-
dc.contributor.localIdA02604-
dc.relation.journalcodeJ02813-
dc.identifier.eissn1976-2437-
dc.identifier.pmid25837192-
dc.subject.keywordE-cadherin-
dc.subject.keywordHypoxia-
dc.subject.keywordZO-1-
dc.subject.keywordpermeability-
dc.contributor.alternativeNameKim, Chang Hoon-
dc.contributor.alternativeNameYoon, Joo Heon-
dc.contributor.affiliatedAuthorKim, Chang Hoon-
dc.contributor.affiliatedAuthorYoon, Joo Heon-
dc.citation.volume56-
dc.citation.number3-
dc.citation.startPage825-
dc.citation.endPage831-
dc.identifier.bibliographicCitationYONSEI MEDICAL JOURNAL, Vol.56(3) : 825-831, 2015-
dc.identifier.rimsid41815-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers

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