Cited 28 times in
Generation of Dopamine Neurons from Rodent Fibroblasts through the Expandable Neural Precursor Cell Stage
DC Field | Value | Language |
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dc.contributor.author | 김동욱 | - |
dc.date.accessioned | 2018-03-26T16:54:17Z | - |
dc.date.available | 2018-03-26T16:54:17Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/156944 | - |
dc.description.abstract | Recent groundbreaking work has demonstrated that combined expression of the transcription factors Brn2, Ascl1, and Myt1L (BAM; also known as Wernig factors) convert mouse fibroblasts into postmitotic neuronal cells. However, questions remain regarding whether trans-conversion is achieved directly or involves an intermediary precursor stage. Trans-conversion toward expandable neural precursor cells (NPCs) is more useful than direct one-step neuron formation with respect to yielding a sufficient number of cells and the feasibility of manipulating NPC differentiation toward certain neuron subtypes. Here, we show that co-expression of Wernig factors and Bcl-xL induces fibroblast conversion into NPCs (induced NPCs (iNPCs)) that are highly expandable for >100 passages. Gene expression analyses showed that the iNPCs exhibited high expression of common NPC genes but not genes specific to defined embryonic brain regions. This finding indicated that a regional identity of iNPCs was not established. Upon induction, iNPCs predominantly differentiated into astrocytes. However, the differentiation potential was not fixed and could be efficiently manipulated into general or specific subtypes of neurons by expression of additional genes. Specifically, overexpression of Nurr1 and Foxa2, transcription factors specific for midbrain dopamine neuron development, drove iNPCs to yield mature midbrain dopamine neurons equipped with presynaptic DA neuronal functions. We further assessed the therapeutic potential of iNPCs in Parkinson disease model rats. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | American Society for Biochemistry and Molecular Biology | - |
dc.relation.isPartOf | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Basic Helix-Loop-Helix Transcription Factors/genetics | - |
dc.subject.MESH | Cell Transdifferentiation* | - |
dc.subject.MESH | Cellular Reprogramming | - |
dc.subject.MESH | Dopamine/metabolism* | - |
dc.subject.MESH | Dopaminergic Neurons/cytology* | - |
dc.subject.MESH | Dopaminergic Neurons/metabolism* | - |
dc.subject.MESH | Fibroblasts/cytology* | - |
dc.subject.MESH | Fibroblasts/metabolism* | - |
dc.subject.MESH | Gene Expression | - |
dc.subject.MESH | Hepatocyte Nuclear Factor 3-beta/genetics | - |
dc.subject.MESH | Mesencephalon/cytology | - |
dc.subject.MESH | Mesencephalon/metabolism | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Inbred C57BL | - |
dc.subject.MESH | Mice, Inbred ICR | - |
dc.subject.MESH | Mice, Transgenic | - |
dc.subject.MESH | Nerve Tissue Proteins/genetics | - |
dc.subject.MESH | Neural Stem Cells/cytology* | - |
dc.subject.MESH | Neural Stem Cells/metabolism* | - |
dc.subject.MESH | Nuclear Receptor Subfamily 4, Group A, Member 2/genetics | - |
dc.subject.MESH | POU Domain Factors/genetics | - |
dc.subject.MESH | Parkinsonian Disorders/metabolism | - |
dc.subject.MESH | Parkinsonian Disorders/pathology | - |
dc.subject.MESH | Parkinsonian Disorders/therapy | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Rats, Inbred Lew | - |
dc.subject.MESH | Rats, Wistar | - |
dc.subject.MESH | Transcription Factors/genetics | - |
dc.title | Generation of Dopamine Neurons from Rodent Fibroblasts through the Expandable Neural Precursor Cell Stage | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Physiology | - |
dc.contributor.googleauthor | Mi-Sun Lim | - |
dc.contributor.googleauthor | Mi-Yoon Chang | - |
dc.contributor.googleauthor | Sang-Mi Kim | - |
dc.contributor.googleauthor | Sang-Hoon Yi | - |
dc.contributor.googleauthor | Haeyoung Suh-Kim | - |
dc.contributor.googleauthor | Sung Jun Jung | - |
dc.contributor.googleauthor | Min Jung Kim | - |
dc.contributor.googleauthor | Jin Hyuk Kim | - |
dc.contributor.googleauthor | Yong-Sung Lee | - |
dc.contributor.googleauthor | Soo Young Lee | - |
dc.contributor.googleauthor | Dong-Wook Kim | - |
dc.contributor.googleauthor | Sang-Hun Lee | - |
dc.contributor.googleauthor | Chang-Hwan Park | - |
dc.identifier.doi | 10.1074/jbc.M114.629808 | - |
dc.contributor.localId | A00406 | - |
dc.relation.journalcode | J01258 | - |
dc.identifier.eissn | 1083-351X | - |
dc.identifier.pmid | 26023233 | - |
dc.subject.keyword | Parkinson disease | - |
dc.subject.keyword | cell therapy | - |
dc.subject.keyword | neural stem cell (NSC) | - |
dc.subject.keyword | neurodegeneration | - |
dc.subject.keyword | reprogramming | - |
dc.contributor.alternativeName | Kim, Dong Wook | - |
dc.contributor.affiliatedAuthor | Kim, Dong Wook | - |
dc.citation.volume | 290 | - |
dc.citation.number | 28 | - |
dc.citation.startPage | 17401 | - |
dc.citation.endPage | 17414 | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, Vol.290(28) : 17401-17414, 2015 | - |
dc.identifier.rimsid | 41253 | - |
dc.type.rims | ART | - |
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