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Staurosporine Induces ROS-Mediated Process Formation in Human Gingival Fibroblasts and Rat Cortical Astrocytes

Authors
 Han Gil Lee  ;  Du Sik Kim  ;  Seong Ah Moon  ;  Jeong Wan Kang  ;  Jeong Taeg Seo 
Citation
 International Journal of Oral Biology, Vol.40(1) : 27-33, 2015 
Journal Title
 International Journal of Oral Biology 
ISSN
 1226-7155 
Issue Date
2015
Keywords
staurosporine ; microtubule ; human gingival fibroblasts ; rat astrocytes
Abstract
In the present study, we investigated the effect of staurosporine on the formation of cellular processes in human gingival fibroblasts and rat astrocytes. Staurosporine caused a rapid induction of process formation in human gingival fibroblasts and rat astrocytes in a concentration dependent manner. The process formation of human gingival fibroblasts and rat astrocytes was prevented by the pretreatment with N-acetylcysteine, suggesting that staurosporine-induced ROS production was responsible for the process formation. Colchicine, a microtubule depolymerizing agent, inhibited the staurosporine-induced process formation, whereas cytochalasin D, an actin filament breakdown agent, failed to suppress the formation of cellular processes. This result indicated that polymerization of microtubule, and not actin filament, was responsible for the formation of cellular processes induced by staurosporine. In support of this hypothesis, Western blot analysis was conducted using anti-tubulin antibody, and the results showed that the amount of polymerized microtubule was increased by the treatment with staurosporine while that of depolymerized beta-tubulin in soluble fraction was decreased. These results indicate that staurosporine induces ROSmediated, microtubule-dependent formation of cellular processes in human gingival fibroblasts and rat astrocytes.
Files in This Item:
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DOI
10.11620/IJOB.2015.40.1.027
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral and Maxillofacial Surgery (구강악안면외과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Kang, Jeong Wan(강정완) ORCID logo https://orcid.org/0000-0002-3945-8452
Moon, Seong Ah(문성아)
Seo, Jeong Taeg(서정택) ORCID logo https://orcid.org/0000-0003-2697-0251
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/156905
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