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Detection of Carbapenemases in Clinical Enterobacteriaceae Isolates Using the VITEK AST-N202 Card

DC FieldValueLanguage
dc.contributor.author이경원-
dc.contributor.author정석훈-
dc.date.accessioned2018-03-26T16:52:24Z-
dc.date.available2018-03-26T16:52:24Z-
dc.date.issued2015-
dc.identifier.issn2093-2340-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/156900-
dc.description.abstractBACKGROUND: The rapid and accurate detection of carbapenemase-producing Enterobacteriaceae (CPE) in clinical microbiology laboratories is essential for the treatment and control of infections caused by these microorganisms. This study was performed to evaluate the ability of the VITEK AST-N202 card to detect CPE isolates. MATERIALS AND METHODS: A total of 43 (Klebsiella pneumoniae, n = 37; Escherichia coli, n = 3; and Enterobacter cloacae, n = 3) CPE isolates and 79 carbapenemase-non-producing Enterobacteriaceae (CNE) isolates were included in this study. The CPE isolates harbored KPC-2 (n = 11), KPC-3 (n = 20), GES-5 (n = 5), VIM-2 (n = 2), IMP-1 (n = 1), NDM-1 (n = 2), or OXA-232 (n = 2). Of the 79 CNE isolates, eight K. pneumoniae isolates were resistant to ertapenem, imipenem, and meropenem, while the remaining 71 isolates were susceptible to the carbapenems. Antimicrobial susceptibilities were tested using the VITEK AST-N202 card, and the results were interpreted as positive when the isolates showed resistant or intermediate results. Modified-Hodge tests (MHTs) were performed using ertapenem or meropenem disks for the screening of carbapenemase production. Polymerase chain reaction (PCR) and direct sequencing were used to identify β-lactamase genes. RESULTS: Sensitivity of MHT with ertapenem and meropenem disks for the detection of carbapenemase was 81.4% (35/43) and 81.4% (35/43), respectively, and a combination with both antibiotic disks increased the sensitivity to 88.4% (38/43). Specificity of the MHT was 100% (79/79) for the CNE isolates. Sensitivity of ertapenem, imipenem, and meropenem as assessed by the VITEK AST-N202 card was 100% (43/43), 93% (40/43), and 95.3% (41/43), respectively. Specificity (89.8%, 71/79) of the test with each carbapenem was improved to 100% (71/71) when eight carbapenem-resistant CNE isolates were excluded from the testing. CONCLUSION: The VITEK AST-N202 card showed high sensitivity for the detection of carbapenemases in Enterobacteriaceae strains. PCR and sequencing experiments for the detection of carbapenemases are recommended when clinical Enterobacteriaceae isolates show non-susceptibility to carbapenems.-
dc.description.statementOfResponsibilityopen-
dc.languageKorean, English-
dc.publisher대한감염학회-
dc.relation.isPartOfINFECTION AND CHEMOTHERAPY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleDetection of Carbapenemases in Clinical Enterobacteriaceae Isolates Using the VITEK AST-N202 Card-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Laboratory Medicine-
dc.contributor.googleauthorIl Kwon Bae-
dc.contributor.googleauthorHyun-Kyung Kang-
dc.contributor.googleauthorIn-Ho Jang-
dc.contributor.googleauthorWoonhyoung Lee-
dc.contributor.googleauthorKeonhan Kim-
dc.contributor.googleauthorJung Ok Kim-
dc.contributor.googleauthorSeok Hoon Jeong-
dc.contributor.googleauthorKyungwon Lee-
dc.identifier.doi10.3947/ic.2015.47.3.167-
dc.contributor.localIdA02649-
dc.contributor.localIdA03619-
dc.relation.journalcodeJ01053-
dc.identifier.eissn2092-6448-
dc.identifier.pmid26483990-
dc.subject.keywordCarbapenemase-producing Enterobacteriaceae-
dc.subject.keywordGES-
dc.subject.keywordKPC-
dc.subject.keywordNDM-
dc.subject.keywordVITEK AST-N202 card-
dc.contributor.alternativeNameLee, Kyung Won-
dc.contributor.alternativeNameJeong, Seok Hoon-
dc.contributor.affiliatedAuthorLee, Kyung Won-
dc.contributor.affiliatedAuthorJeong, Seok Hoon-
dc.citation.volume47-
dc.citation.number3-
dc.citation.startPage167-
dc.citation.endPage174-
dc.identifier.bibliographicCitationINFECTION AND CHEMOTHERAPY, Vol.47(3) : 167-174, 2015-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers

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