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Epstein-Barr virus BARF1-induced NFκB/miR-146a/SMAD4 alterations in stomach cancer cells
DC Field | Value | Language |
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dc.contributor.author | 라선영 | - |
dc.date.accessioned | 2018-01-23T05:54:03Z | - |
dc.date.available | 2018-01-23T05:54:03Z | - |
dc.date.issued | 2016 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/155795 | - |
dc.description.abstract | Epstein-Barr virus (EBV)-encoded BamHI-A rightward frame 1 (BARF1) is a putative viral oncogene in EBV-infected stomach cancer. The aim of the present study was to investigate BARF1-induced cellular protein and microRNA alterations. In this study, BARF1-expressing stomach cancer cells showed a high rate of proliferation, high levels of NFκB, and miR-146a upregulation, which was reversed by NFκB knockdown. During BARF1-induced NFκB upregulation, hCSF1 receptor level was unchanged. Knockdown of BARF1 in the naturally EBV-infected YCCEL1 stomach cancer cells suppressed cell proliferation, and downregulated NFκB and miR-146a. SMAD4 was identified as a miR-146a target and was downregulated in BARF1-expressing cells, whereas SMAD4 expression was restored by anti-miR-146a. Knockdown of BARF1 in YCCEL1 cells upregulated SMAD4, and this effect was reversed by miR-146a overexpression. Transfection of BARF1-expressing cells with pCEP4-SMAD4 abolished the cell proliferating effect of BARF1. In stomach cancer tissues, miR-146a was expressed at higher levels, and more frequent NFκB nuclear positivity immunohistochemically, but not of SMAD4 nuclear loss was found in the EBV-positive group compared with the EBV-negative group. In conclusion, EBV-encoded BARF1 promotes cell proliferation in stomach cancer by upregulating NFκB and miR-146a and downregulating SMAD4, thereby contributing to EBV-induced stomach cancer progression. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | ONCOTARGET | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Epstein-Barr virus BARF1-induced NFκB/miR-146a/SMAD4 alterations in stomach cancer cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Internal Medicine | - |
dc.contributor.googleauthor | Dong Ha Kim | - |
dc.contributor.googleauthor | Mee Soo Chang | - |
dc.contributor.googleauthor | Chan Jin Yoon | - |
dc.contributor.googleauthor | Jaap M. Middeldorp | - |
dc.contributor.googleauthor | Olivia M. Martinez | - |
dc.contributor.googleauthor | Sun-ju Byeon | - |
dc.contributor.googleauthor | Sun Young Rha | - |
dc.contributor.googleauthor | Sung Han Kim | - |
dc.contributor.googleauthor | Yang Soo Kim | - |
dc.contributor.googleauthor | Jun Hee Woo | - |
dc.identifier.doi | 10.18632/oncotarget.10511 | - |
dc.contributor.localId | A01316 | - |
dc.relation.journalcode | J02421 | - |
dc.identifier.eissn | 1949-2553 | - |
dc.identifier.pmid | 27438138 | - |
dc.contributor.alternativeName | Rha, Sun Young | - |
dc.contributor.affiliatedAuthor | Rha, Sun Young | - |
dc.citation.volume | 7 | - |
dc.citation.number | 50 | - |
dc.citation.startPage | 82213 | - |
dc.citation.endPage | 82227 | - |
dc.identifier.bibliographicCitation | ONCOTARGET , Vol.7(50) : 82213-82227, 2016 | - |
dc.identifier.rimsid | 48825 | - |
dc.type.rims | ART | - |
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