0 251

Cited 0 times in

Behavioral, metabolic and functional brain changes in a rat model of chronic neuropathic pain: a longitudinal MRI study

Authors
 Catherine S. Hubbard  ;  Shariq A. Khan  ;  Su Xu  ;  Myeounghoon Cha  ;  Radi Masri  ;  David A. Seminowicz 
Citation
 NEUROIMAGE, Vol.107 : 333-344, 2015 
Journal Title
NEUROIMAGE
ISSN
 1053-8119 
Issue Date
2015
MeSH
Animals ; Anxiety/psychology ; Behavior, Animal/physiology* ; Brain Chemistry* ; Chronic Disease ; Cold Temperature ; Exploratory Behavior ; Female ; Gyrus Cinguli ; Longitudinal Studies ; Magnetic Resonance Imaging ; Neuralgia/metabolism ; Neuralgia/pathology* ; Neuralgia/psychology* ; Peripheral Nervous System Diseases/metabolism ; Peripheral Nervous System Diseases/pathology ; Peripheral Nervous System Diseases/psychology ; Physical Stimulation ; Rats ; Rats, Sprague-Dawley
Abstract
Peripheral neuropathy often manifests clinically with symptoms of mechanical and cold allodynia. However, the neuroplastic changes associated with peripheral neuropathic pain and the onset and progression of allodynic symptoms remain unclear. Here, we used a chronic neuropathic pain model (spared nerve injury; SNI) to examine functional and metabolic brain changes associated with the development and maintenance of mechanical and cold hypersensitivity, the latter which we assessed both behaviorally and during a novel acetone application paradigm using functional MRI (fMRI). Female Sprague-Dawley rats underwent SNI (n=7) or sham (n=5) surgery to the left hindpaw. Rats were anesthetized and scanned using a 7 T MRI scanner 1 week prior to (pre-injury) and 4 (early/subchronic) and 20 weeks (late/chronic) post-injury. Functional scans were acquired during acetone application to the left hindpaw. (1)H magnetic resonance spectroscopy was also performed to assess SNI-induced metabolic changes in the anterior cingulate cortex (ACC) pre- and 4 weeks post-injury. Mechanical and cold sensitivity, as well as anxiety-like behaviors, were assessed 2 weeks pre-injury, and 2, 5, 9, 14, and 19 weeks post-injury. Stimulus-evoked brain responses (acetone application to the left hindpaw) were analyzed across the pre- and post-injury time points. In response to acetone application during fMRI, SNI rats showed widespread and functionally diverse changes within pain-related brain regions including somatosensory and cingulate cortices and subcortically within the thalamus and the periaqueductal gray. These functional brain changes temporally coincided with early and sustained increases in both mechanical and cold sensitivity. SNI rats also showed increased glutamate within the ACC that correlated with behavioral measures of cold hypersensitivity. Together, our findings suggest that extensive functional reorganization within pain-related brain regions may underlie the development and chronification of allodynic-like behaviors.
Full Text
http://www.sciencedirect.com/science/article/pii/S1053811914010167
DOI
10.1016/j.neuroimage.2014.12.024
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Physiology (생리학교실) > 1. Journal Papers
Yonsei Authors
Cha, Myeoung Hoon(차명훈) ORCID logo https://orcid.org/0000-0002-7993-672X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/155699
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links