Cited 37 times in
Synaptopodin Is a Coincidence Detector of Tyrosine versus Serine/Threonine Phosphorylation for the Modulation of Rho Protein Crosstalk in Podocytes
DC Field | Value | Language |
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dc.contributor.author | 최훈영 | - |
dc.date.accessioned | 2017-11-02T08:39:15Z | - |
dc.date.available | 2017-11-02T08:39:15Z | - |
dc.date.issued | 2017 | - |
dc.identifier.issn | 1046-6673 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/154722 | - |
dc.description.abstract | Tyrosine and serine/threonine signal-transduction pathways influence many aspects of cell behavior, including the spatial and temporal regulation of the actin cytoskeleton. However, little is known about how input from diverse tyrosine and serine/threonine kinases is integrated to control Rho protein crosstalk and actin remodeling, which are critically important in podocyte health and disease. Here we unveil the proteolytically-regulated, actin organizing protein synaptopodin as a coincidence detector of tyrosine versus serine/threonine phosphorylation. We show that serine/threonine and tyrosine kinases duel for synaptopodin stability versus degradation. EGFR/Src-mediated tyrosine phosphorylation of synaptopodin in podocytes promotes binding to the serine/threonine phosphatase calcineurin. This leads to the loss of 14-3-3 binding, resulting in synaptopodin degradation, Vav2 activation, enhanced Rac1 signaling, and ultimate loss of stress fibers. Our studies reveal how synaptopodin, a single proteolytically-controlled protein, integrates antagonistic tyrosine versus serine/threonine phosphorylation events for the dynamic control of the actin cytoskeleton in podocytes. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | American Society of Nephrology | - |
dc.relation.isPartOf | JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Calcineurin/metabolism | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Microfilament Proteins/physiology* | - |
dc.subject.MESH | Phosphorylation | - |
dc.subject.MESH | Podocytes/physiology* | - |
dc.subject.MESH | Receptor Cross-Talk | - |
dc.subject.MESH | Serine/metabolism* | - |
dc.subject.MESH | Signal Transduction | - |
dc.subject.MESH | Threonine/metabolism* | - |
dc.subject.MESH | Tyrosine/metabolism* | - |
dc.subject.MESH | rhoA GTP-Binding Protein/physiology* | - |
dc.title | Synaptopodin Is a Coincidence Detector of Tyrosine versus Serine/Threonine Phosphorylation for the Modulation of Rho Protein Crosstalk in Podocytes | - |
dc.type | Article | - |
dc.publisher.location | United States | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Internal Medicine | - |
dc.contributor.googleauthor | Lisa Buvall | - |
dc.contributor.googleauthor | Hanna Wallentin | - |
dc.contributor.googleauthor | Jonas Sieber | - |
dc.contributor.googleauthor | Svetlana Andreeva | - |
dc.contributor.googleauthor | Hoon Young Choi | - |
dc.contributor.googleauthor | Peter Mundel | - |
dc.contributor.googleauthor | Anna Greka | - |
dc.identifier.doi | 10.1681/ASN.2016040414 | - |
dc.contributor.localId | A04226 | - |
dc.relation.journalcode | J01779 | - |
dc.identifier.eissn | 1533-3450 | - |
dc.identifier.pmid | 27628902 | - |
dc.identifier.url | http://jasn.asnjournals.org/content/28/3/837.long | - |
dc.subject.keyword | EGFR | - |
dc.subject.keyword | FSGS | - |
dc.subject.keyword | Rac1 | - |
dc.subject.keyword | TRPC5 | - |
dc.subject.keyword | cytoskeleton | - |
dc.subject.keyword | glomerular disease | - |
dc.contributor.alternativeName | Choi, Hoon Young | - |
dc.contributor.affiliatedAuthor | Choi, Hoon Young | - |
dc.citation.title | Journal of the American Society of Nephrology | - |
dc.citation.volume | 28 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 837 | - |
dc.citation.endPage | 851 | - |
dc.identifier.bibliographicCitation | JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, Vol.28(3) : 837-851, 2017 | - |
dc.date.modified | 2017-11-01 | - |
dc.identifier.rimsid | 44203 | - |
dc.type.rims | ART | - |
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