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Sorafenib inhibits 5-fluorouracil-resistant gastric cancer cell growth.

DC FieldValueLanguage
dc.contributor.author송시영-
dc.contributor.author김선아-
dc.date.accessioned2017-11-02T08:38:48Z-
dc.date.available2017-11-02T08:38:48Z-
dc.date.issued2017-
dc.identifier.issn0036-5521-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/154711-
dc.description.abstractBACKGROUND: Sorafenib is a multi-kinase inhibitor used in the treatment of various cancers. This study investigated the inhibitory effect of sorafenib on xenograft models of gastric cancer cells and 5-fluorouracil (5-FU)-resistant cells. METHODS: The half-maximal inhibitory concentration (IC50) of sorafenib in NCI-N87 cells was determined. Xenograft models were established using BALB/c nude mice and were divided into four groups treated with vehicle, sorafenib (20 mg kg-1 day-1), 5-FU (50 mg kg-1 week-1), or a combination of sorafenib (20 mg kg-1 day-1) plus 5-FU (50 mg kg-1 week-1). 5-FU-resistant NCI-N87 cells were established by repeated exposure to 5-FU. RESULTS: Sorafenib inhibited NCI-N87 cell growth in a concentration-dependent manner with a mean IC50 of 16.345 ± 5.391 μM. Phosphorylation levels of mitogen-activated protein kinase kinase and extracellular signal-regulated kinase in these cells decreased in a dose-dependent manner after exposure to sorafenib. Sorafenib induced the activation of caspase-3, and its combination with 5-FU more effectively inhibited the growth of xenograft tumors than either sorafenib or 5-FU alone (p < 0.05). Sorafenib markedly inhibited 5-FU-resistant NCI-N87 cell growth as well as sphere formation in both parental and 5-FU-resistant NCI-N87 cells. CONCLUSIONS: The sorafenib and 5-FU combination exhibited enhanced antitumor effects in a gastric cancer xenograft model and inhibited 5-FU-resistant cell proliferation and sphere formation. These findings suggest that sorafenib is useful in overcoming gastric cancer resistance to conventional chemotherapy.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherInforma Healthcare-
dc.relation.isPartOfSCANDINAVIAN JOURNAL OF GASTROENTEROLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHAntineoplastic Combined Chemotherapy Protocols/administration & dosage*-
dc.subject.MESHApoptosis-
dc.subject.MESHCaspase 3/metabolism-
dc.subject.MESHCell Cycle/drug effects-
dc.subject.MESHCell Line, Tumor-
dc.subject.MESHCell Proliferation-
dc.subject.MESHDrug Resistance, Neoplasm-
dc.subject.MESHExtracellular Signal-Regulated MAP Kinases/metabolism-
dc.subject.MESHFemale-
dc.subject.MESHFluorouracil/administration & dosage*-
dc.subject.MESHHumans-
dc.subject.MESHMice-
dc.subject.MESHMice, Inbred BALB C-
dc.subject.MESHMice, Nude-
dc.subject.MESHNiacinamide/administration & dosage-
dc.subject.MESHNiacinamide/analogs & derivatives*-
dc.subject.MESHPhenylurea Compounds/administration & dosage*-
dc.subject.MESHStomach/pathology-
dc.subject.MESHStomach Neoplasms/drug therapy*-
dc.subject.MESHStomach Neoplasms/pathology-
dc.subject.MESHXenograft Model Antitumor Assays-
dc.titleSorafenib inhibits 5-fluorouracil-resistant gastric cancer cell growth.-
dc.typeArticle-
dc.publisher.locationEngland-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Internal Medicine-
dc.contributor.googleauthorHee Man Kim-
dc.contributor.googleauthorSun A Kim-
dc.contributor.googleauthorSoo Been Park-
dc.contributor.googleauthorJae Hee Cho-
dc.contributor.googleauthorSi Young Song-
dc.identifier.doi10.1080/00365521.2017.1278786-
dc.contributor.localIdA02035-
dc.relation.journalcodeJ02632-
dc.identifier.eissn1502-7708-
dc.identifier.pmid28110575-
dc.identifier.urlhttp://www.tandfonline.com/doi/full/10.1080/00365521.2017.1278786-
dc.subject.keyword5-fluorouracil-
dc.subject.keywordGastric cancer-
dc.subject.keyworddrug resistance-
dc.subject.keywordneoplastic stem cell-
dc.subject.keywordsorafenib-
dc.subject.keywordsphere formation-
dc.contributor.alternativeNameSong, Si Young-
dc.contributor.affiliatedAuthorSong, Si Young-
dc.citation.titleScandinavian Journal of Gastroenterology-
dc.citation.volume52-
dc.citation.number5-
dc.citation.startPage577-
dc.citation.endPage584-
dc.identifier.bibliographicCitationSCANDINAVIAN JOURNAL OF GASTROENTEROLOGY, Vol.52(5) : 577-584, 2017-
dc.date.modified2017-11-01-
dc.identifier.rimsid44187-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

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