Cited 8 times in
Human MicroRNA Responses Predict Cytomegalovirus Replication Following Solid Organ Transplantation
DC Field | Value | Language |
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dc.contributor.author | 한상훈 | - |
dc.date.accessioned | 2017-11-02T08:34:32Z | - |
dc.date.available | 2017-11-02T08:34:32Z | - |
dc.date.issued | 2017 | - |
dc.identifier.issn | 0022-1899 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/154629 | - |
dc.description.abstract | BACKGROUND: Homo sapiens mature micro-ribonucleic acid (miRNA)-200b-3p and 200c-3p are predicted to bind to 3' untranslated region of mRNA encoding human cytomegalovirus (HCMV) immediate early protein 2 (IE2). We hypothesized that expression of these miRNAs pretransplant could predict HCMV replication after solid organ transplantation (SOT). METHODS: A total of 272 SOT recipients were HCMV-seropositive pretransplant and were managed using preemptive therapy. Pretransplant peripheral blood mononuclear cells were stimulated with HCMV followed by collection of RNA 1 day poststimulation. The miRNAs were quantified using real-time reverse transcription-polymerase chain reaction. Human foreskin fibroblasts were transfected with 200b-3p and 200c-3p and infected with HCMV 1 hour post-transfection. Protein was collected at 3 days postinfection (dpi) and 7 dpi underwent immunoblotting for IE2. RESULTS: Medians of 200b-3p and 200c-3p were significantly lower in recipients with HCMV replication (n = 144) (361.6 vs 552.6, P = .035; 3586.8 vs 12986.8 copies/μL, P = .03, respectively). Multivariate regression revealed that 200b-3p ≥100 copies/μL (odds ratio [OR]: 0.53; P = .02), was associated with less HCMV replication. Transfection with 200b-3p resulted in 2.7- and 2.5-fold decreased IE2 at 3 dpi and 7 dpi, respectively, compared to mock cells. CONCLUSIONS: MicroRNAs may play a biologically relevant role in controlling HCMV replication post-transplant. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Oxford University Press | - |
dc.relation.isPartOf | JOURNAL OF INFECTIOUS DISEASES | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adult | - |
dc.subject.MESH | Aged | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Cytomegalovirus/physiology* | - |
dc.subject.MESH | Cytomegalovirus Infections/virology* | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Follow-Up Studies | - |
dc.subject.MESH | HeLa Cells | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Immediate-Early Proteins/genetics | - |
dc.subject.MESH | Immediate-Early Proteins/metabolism | - |
dc.subject.MESH | Leukocytes, Mononuclear/metabolism | - |
dc.subject.MESH | Leukocytes, Mononuclear/virology | - |
dc.subject.MESH | Logistic Models | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | MicroRNAs/genetics | - |
dc.subject.MESH | MicroRNAs/metabolism* | - |
dc.subject.MESH | Middle Aged | - |
dc.subject.MESH | Multivariate Analysis | - |
dc.subject.MESH | Organ Transplantation* | - |
dc.subject.MESH | Prospective Studies | - |
dc.subject.MESH | RNA, Messenger/genetics | - |
dc.subject.MESH | RNA, Messenger/metabolism | - |
dc.subject.MESH | Reproducibility of Results | - |
dc.subject.MESH | Virus Replication* | - |
dc.title | Human MicroRNA Responses Predict Cytomegalovirus Replication Following Solid Organ Transplantation | - |
dc.type | Article | - |
dc.publisher.location | United States | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Internal Medicine | - |
dc.contributor.googleauthor | Sang Hoon Han | - |
dc.contributor.googleauthor | Deepali Kumar | - |
dc.contributor.googleauthor | Victor H. Ferreira | - |
dc.contributor.googleauthor | Adrian Egli | - |
dc.contributor.googleauthor | Hans H. Hirsch | - |
dc.contributor.googleauthor | Maja Weisser | - |
dc.contributor.googleauthor | Christian Garzoni | - |
dc.contributor.googleauthor | Christian van Delden | - |
dc.contributor.googleauthor | Pierre-Yves Bochud | - |
dc.contributor.googleauthor | Oriol Manuel | - |
dc.contributor.googleauthor | Pascal Meylan | - |
dc.contributor.googleauthor | Katia Boggian | - |
dc.contributor.googleauthor | Shahid Husain | - |
dc.contributor.googleauthor | Nicolas J. Mueller | - |
dc.contributor.googleauthor | Atul Humar | - |
dc.contributor.googleauthor | Swiss Transplant Cohort Study | - |
dc.identifier.doi | 10.1093/infdis/jiw596 | - |
dc.contributor.localId | A04286 | - |
dc.relation.journalcode | J01454 | - |
dc.identifier.eissn | 1537-6613 | - |
dc.identifier.pmid | 28003351 | - |
dc.identifier.url | https://academic.oup.com/jid/article-lookup/doi/10.1093/infdis/jiw596 | - |
dc.subject.keyword | cytomegalovirus | - |
dc.subject.keyword | microRNA | - |
dc.subject.keyword | solid organ transplant | - |
dc.subject.keyword | immediate early protein | - |
dc.contributor.alternativeName | Han, Sang Hoon | - |
dc.contributor.affiliatedAuthor | Han, Sang Hoon | - |
dc.citation.title | Journal of Infectious Diseases | - |
dc.citation.volume | 215 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 537 | - |
dc.citation.endPage | 546 | - |
dc.identifier.bibliographicCitation | JOURNAL OF INFECTIOUS DISEASES, Vol.215(4) : 537-546, 2017 | - |
dc.date.modified | 2017-11-01 | - |
dc.identifier.rimsid | 43687 | - |
dc.type.rims | ART | - |
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