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Human MicroRNA Responses Predict Cytomegalovirus Replication Following Solid Organ Transplantation

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dc.contributor.author한상훈-
dc.date.accessioned2017-11-02T08:34:32Z-
dc.date.available2017-11-02T08:34:32Z-
dc.date.issued2017-
dc.identifier.issn0022-1899-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/154629-
dc.description.abstractBACKGROUND: Homo sapiens mature micro-ribonucleic acid (miRNA)-200b-3p and 200c-3p are predicted to bind to 3' untranslated region of mRNA encoding human cytomegalovirus (HCMV) immediate early protein 2 (IE2). We hypothesized that expression of these miRNAs pretransplant could predict HCMV replication after solid organ transplantation (SOT). METHODS: A total of 272 SOT recipients were HCMV-seropositive pretransplant and were managed using preemptive therapy. Pretransplant peripheral blood mononuclear cells were stimulated with HCMV followed by collection of RNA 1 day poststimulation. The miRNAs were quantified using real-time reverse transcription-polymerase chain reaction. Human foreskin fibroblasts were transfected with 200b-3p and 200c-3p and infected with HCMV 1 hour post-transfection. Protein was collected at 3 days postinfection (dpi) and 7 dpi underwent immunoblotting for IE2. RESULTS: Medians of 200b-3p and 200c-3p were significantly lower in recipients with HCMV replication (n = 144) (361.6 vs 552.6, P = .035; 3586.8 vs 12986.8 copies/μL, P = .03, respectively). Multivariate regression revealed that 200b-3p ≥100 copies/μL (odds ratio [OR]: 0.53; P = .02), was associated with less HCMV replication. Transfection with 200b-3p resulted in 2.7- and 2.5-fold decreased IE2 at 3 dpi and 7 dpi, respectively, compared to mock cells. CONCLUSIONS: MicroRNAs may play a biologically relevant role in controlling HCMV replication post-transplant.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherOxford University Press-
dc.relation.isPartOfJOURNAL OF INFECTIOUS DISEASES-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAdult-
dc.subject.MESHAged-
dc.subject.MESHCell Line-
dc.subject.MESHCytomegalovirus/physiology*-
dc.subject.MESHCytomegalovirus Infections/virology*-
dc.subject.MESHFemale-
dc.subject.MESHFollow-Up Studies-
dc.subject.MESHHeLa Cells-
dc.subject.MESHHumans-
dc.subject.MESHImmediate-Early Proteins/genetics-
dc.subject.MESHImmediate-Early Proteins/metabolism-
dc.subject.MESHLeukocytes, Mononuclear/metabolism-
dc.subject.MESHLeukocytes, Mononuclear/virology-
dc.subject.MESHLogistic Models-
dc.subject.MESHMale-
dc.subject.MESHMicroRNAs/genetics-
dc.subject.MESHMicroRNAs/metabolism*-
dc.subject.MESHMiddle Aged-
dc.subject.MESHMultivariate Analysis-
dc.subject.MESHOrgan Transplantation*-
dc.subject.MESHProspective Studies-
dc.subject.MESHRNA, Messenger/genetics-
dc.subject.MESHRNA, Messenger/metabolism-
dc.subject.MESHReproducibility of Results-
dc.subject.MESHVirus Replication*-
dc.titleHuman MicroRNA Responses Predict Cytomegalovirus Replication Following Solid Organ Transplantation-
dc.typeArticle-
dc.publisher.locationUnited States-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Internal Medicine-
dc.contributor.googleauthorSang Hoon Han-
dc.contributor.googleauthorDeepali Kumar-
dc.contributor.googleauthorVictor H. Ferreira-
dc.contributor.googleauthorAdrian Egli-
dc.contributor.googleauthorHans H. Hirsch-
dc.contributor.googleauthorMaja Weisser-
dc.contributor.googleauthorChristian Garzoni-
dc.contributor.googleauthorChristian van Delden-
dc.contributor.googleauthorPierre-Yves Bochud-
dc.contributor.googleauthorOriol Manuel-
dc.contributor.googleauthorPascal Meylan-
dc.contributor.googleauthorKatia Boggian-
dc.contributor.googleauthorShahid Husain-
dc.contributor.googleauthorNicolas J. Mueller-
dc.contributor.googleauthorAtul Humar-
dc.contributor.googleauthorSwiss Transplant Cohort Study-
dc.identifier.doi10.1093/infdis/jiw596-
dc.contributor.localIdA04286-
dc.relation.journalcodeJ01454-
dc.identifier.eissn1537-6613-
dc.identifier.pmid28003351-
dc.identifier.urlhttps://academic.oup.com/jid/article-lookup/doi/10.1093/infdis/jiw596-
dc.subject.keywordcytomegalovirus-
dc.subject.keywordmicroRNA-
dc.subject.keywordsolid organ transplant-
dc.subject.keywordimmediate early protein-
dc.contributor.alternativeNameHan, Sang Hoon-
dc.contributor.affiliatedAuthorHan, Sang Hoon-
dc.citation.titleJournal of Infectious Diseases-
dc.citation.volume215-
dc.citation.number4-
dc.citation.startPage537-
dc.citation.endPage546-
dc.identifier.bibliographicCitationJOURNAL OF INFECTIOUS DISEASES, Vol.215(4) : 537-546, 2017-
dc.date.modified2017-11-01-
dc.identifier.rimsid43687-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

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