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Clinical Usefulness of a One-Tube Nested Reverse Transcription Quantitative Polymerase Chain Reaction Assay for Evaluating Human Epidermal Growth Factor Receptor 2 mRNA Overexpression in Formalin-Fixed and Paraffin-Embedded Breast Cancer Tissue Samples

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dc.contributor.author김승일-
dc.date.accessioned2017-11-02T08:26:29Z-
dc.date.available2017-11-02T08:26:29Z-
dc.date.issued2017-
dc.identifier.issn1015-2008-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/154470-
dc.description.abstractBACKGROUND: Currently, the two main methods used to analyze human epidermal growth factor receptor 2 (HER2) amplification or overexpression have a limited accuracy and high costs. These limitations can be overcome by the development of complementary quantitative methods. METHODS: In this study, we analyzed HER2 mRNA expression in clinical formalin-fixed and paraffin-embedded (FFPE) samples using a one-tube nested reverse transcription quantitative polymerase chain reaction (RT-qPCR) assay. We measured expression relative to 3 reference genes and compared the results to those obtained by conventional immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) assays with 226 FFPE breast cancer tissue samples. RESULTS: The one-tube nested RT-qPCR assay proved to be highly sensitive and specific based on comparisons with IHC (96.9 and 97.7%, respectively) and FISH (92.4 and 92.9%, respectively) obtained with the validation set. Comparisons with clinicopathological data revealed significant associations between HER2 overexpression and TNM stage (p < 0.01), histological type (p < 0.01), ER status (p < 0.001), PR status (p < 0.05), HER2 status (p < 0.001), and molecular subtypes (p < 0.001). CONCLUSION: Based on these findings, our one-tube nested RT-qPCR assay is a potentially useful and complementary screening tool for the detection of HER2 mRNA overexpression.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherS. Karger-
dc.relation.isPartOfPATHOBIOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHBiomarkers, Tumor-
dc.subject.MESHBreast/metabolism*-
dc.subject.MESHBreast/pathology-
dc.subject.MESHBreast Neoplasms/genetics*-
dc.subject.MESHBreast Neoplasms/metabolism-
dc.subject.MESHBreast Neoplasms/pathology-
dc.subject.MESHCell Line, Tumor-
dc.subject.MESHFemale-
dc.subject.MESHFormaldehyde-
dc.subject.MESHHumans-
dc.subject.MESHImmunohistochemistry-
dc.subject.MESHIn Situ Hybridization, Fluorescence-
dc.subject.MESHParaffin Embedding-
dc.subject.MESHReal-Time Polymerase Chain Reaction-
dc.subject.MESHReceptor, ErbB-2/genetics*-
dc.subject.MESHReceptor, ErbB-2/metabolism-
dc.subject.MESHSensitivity and Specificity-
dc.subject.MESHTissue Fixation-
dc.titleClinical Usefulness of a One-Tube Nested Reverse Transcription Quantitative Polymerase Chain Reaction Assay for Evaluating Human Epidermal Growth Factor Receptor 2 mRNA Overexpression in Formalin-Fixed and Paraffin-Embedded Breast Cancer Tissue Samples-
dc.typeArticle-
dc.publisher.locationSwitzerland-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Surgery-
dc.contributor.googleauthorWang H.a-
dc.contributor.googleauthorAhn S.b-
dc.contributor.googleauthorPark S.b-
dc.contributor.googleauthorKim S.c-
dc.contributor.googleauthorLee H.b-
dc.identifier.doi10.1159/000447301-
dc.contributor.localIdA00658-
dc.relation.journalcodeJ02470-
dc.identifier.eissn1423-0291-
dc.identifier.pmid27544315-
dc.identifier.urlhttps://www.karger.com/Article/Abstract/447301-
dc.subject.keywordHuman epidermal growth factor receptor 2 overexpression-
dc.subject.keywordMolecular diagnosis-
dc.subject.keywordImmunohistochemistry-
dc.subject.keywordFluorescence in situ hybridization-
dc.subject.keywordOne-tube nested reverse transcription quantitative polymerase chain reaction-
dc.subject.keywordBreast cancer-
dc.contributor.alternativeNameKim, Seung Il-
dc.contributor.affiliatedAuthorKim, Seung Il-
dc.citation.titlePathobiology-
dc.citation.volume84-
dc.citation.number2-
dc.citation.startPage57-
dc.citation.endPage70-
dc.identifier.bibliographicCitationPATHOBIOLOGY, Vol.84(2) : 57-70, 2017-
dc.date.modified2017-11-01-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers

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