Cited 2 times in
Clinical Usefulness of a One-Tube Nested Reverse Transcription Quantitative Polymerase Chain Reaction Assay for Evaluating Human Epidermal Growth Factor Receptor 2 mRNA Overexpression in Formalin-Fixed and Paraffin-Embedded Breast Cancer Tissue Samples
DC Field | Value | Language |
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dc.contributor.author | 김승일 | - |
dc.date.accessioned | 2017-11-02T08:26:29Z | - |
dc.date.available | 2017-11-02T08:26:29Z | - |
dc.date.issued | 2017 | - |
dc.identifier.issn | 1015-2008 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/154470 | - |
dc.description.abstract | BACKGROUND: Currently, the two main methods used to analyze human epidermal growth factor receptor 2 (HER2) amplification or overexpression have a limited accuracy and high costs. These limitations can be overcome by the development of complementary quantitative methods. METHODS: In this study, we analyzed HER2 mRNA expression in clinical formalin-fixed and paraffin-embedded (FFPE) samples using a one-tube nested reverse transcription quantitative polymerase chain reaction (RT-qPCR) assay. We measured expression relative to 3 reference genes and compared the results to those obtained by conventional immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) assays with 226 FFPE breast cancer tissue samples. RESULTS: The one-tube nested RT-qPCR assay proved to be highly sensitive and specific based on comparisons with IHC (96.9 and 97.7%, respectively) and FISH (92.4 and 92.9%, respectively) obtained with the validation set. Comparisons with clinicopathological data revealed significant associations between HER2 overexpression and TNM stage (p < 0.01), histological type (p < 0.01), ER status (p < 0.001), PR status (p < 0.05), HER2 status (p < 0.001), and molecular subtypes (p < 0.001). CONCLUSION: Based on these findings, our one-tube nested RT-qPCR assay is a potentially useful and complementary screening tool for the detection of HER2 mRNA overexpression. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | S. Karger | - |
dc.relation.isPartOf | PATHOBIOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Biomarkers, Tumor | - |
dc.subject.MESH | Breast/metabolism* | - |
dc.subject.MESH | Breast/pathology | - |
dc.subject.MESH | Breast Neoplasms/genetics* | - |
dc.subject.MESH | Breast Neoplasms/metabolism | - |
dc.subject.MESH | Breast Neoplasms/pathology | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Formaldehyde | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Immunohistochemistry | - |
dc.subject.MESH | In Situ Hybridization, Fluorescence | - |
dc.subject.MESH | Paraffin Embedding | - |
dc.subject.MESH | Real-Time Polymerase Chain Reaction | - |
dc.subject.MESH | Receptor, ErbB-2/genetics* | - |
dc.subject.MESH | Receptor, ErbB-2/metabolism | - |
dc.subject.MESH | Sensitivity and Specificity | - |
dc.subject.MESH | Tissue Fixation | - |
dc.title | Clinical Usefulness of a One-Tube Nested Reverse Transcription Quantitative Polymerase Chain Reaction Assay for Evaluating Human Epidermal Growth Factor Receptor 2 mRNA Overexpression in Formalin-Fixed and Paraffin-Embedded Breast Cancer Tissue Samples | - |
dc.type | Article | - |
dc.publisher.location | Switzerland | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Surgery | - |
dc.contributor.googleauthor | Wang H.a | - |
dc.contributor.googleauthor | Ahn S.b | - |
dc.contributor.googleauthor | Park S.b | - |
dc.contributor.googleauthor | Kim S.c | - |
dc.contributor.googleauthor | Lee H.b | - |
dc.identifier.doi | 10.1159/000447301 | - |
dc.contributor.localId | A00658 | - |
dc.relation.journalcode | J02470 | - |
dc.identifier.eissn | 1423-0291 | - |
dc.identifier.pmid | 27544315 | - |
dc.identifier.url | https://www.karger.com/Article/Abstract/447301 | - |
dc.subject.keyword | Human epidermal growth factor receptor 2 overexpression | - |
dc.subject.keyword | Molecular diagnosis | - |
dc.subject.keyword | Immunohistochemistry | - |
dc.subject.keyword | Fluorescence in situ hybridization | - |
dc.subject.keyword | One-tube nested reverse transcription quantitative polymerase chain reaction | - |
dc.subject.keyword | Breast cancer | - |
dc.contributor.alternativeName | Kim, Seung Il | - |
dc.contributor.affiliatedAuthor | Kim, Seung Il | - |
dc.citation.title | Pathobiology | - |
dc.citation.volume | 84 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 57 | - |
dc.citation.endPage | 70 | - |
dc.identifier.bibliographicCitation | PATHOBIOLOGY, Vol.84(2) : 57-70, 2017 | - |
dc.date.modified | 2017-11-01 | - |
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