Cited 17 times in
Isoflurane preconditioning inhibits the effects of tissue-type plasminogen activator on brain endothelial cell in an in vitro model of ischemic stroke
DC Field | Value | Language |
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dc.contributor.author | 구본녀 | - |
dc.contributor.author | 김소연 | - |
dc.contributor.author | 김은정 | - |
dc.contributor.author | 김정민 | - |
dc.contributor.author | 이재훈 | - |
dc.date.accessioned | 2017-11-02T08:20:43Z | - |
dc.date.available | 2017-11-02T08:20:43Z | - |
dc.date.issued | 2017 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/154361 | - |
dc.description.abstract | Tissue-type plasminogen activator (tPA) is the only treatment for ischemic stroke. However, tPA could induce the intracranial hemorrhage (ICH), which is the main cause of death in ischemic stroke patient after tPA treatment. At present, there is no treatment strategy to ameliorate tPA-induced brain injury after ischemia. Therefore, we investigated the effect of pre-treated isoflurane, which is a volatile anesthetic and has beneficial effects on neurological dysfunction, brain edema and infarct volume in ischemic stroke model. In this study, we used oxygen/glucose deprivation and reperfusion (OGD/R) condition to mimic an ischemic stroke in vitro. Matrix metalloproteinases (MMP) activity was measured in endothelial cell media. Also, neuronal cell culture was performed to investigate the effect of pretreated isoflurane on the neuronal cell survival after tPA-induced injury during OGD/R. Isoflurane pretreatment prevented tPA-induced MMP-2 and MMP-9 activity and suppressed tPA-triggered LRP/NF-κB/Cox-2 signaling after OGD/R. Neuronal cells, incubated with endothelial cell conditioned medium (EC-CM) after tPA + OGD/R, showed upregulation of pro-apoptotic molecules. However, neurons incubated with isoflurane-pretreated EC-CM showed increased anti-apoptotic molecules. Our findings suggest that isoflurane pretreatment could attenuate tPA-exaggerated brain ischemic injury, by reducing tPA-induced LRP/NF-κB/Cox-2 in endothelial cells, endothelial MMP-2 and MMP-9 activation, and subsequent pro-apoptotic molecule in neurons after OGD/R. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Ivyspring International Publisher | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF MEDICAL SCIENCES | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Apoptosis/drug effects | - |
dc.subject.MESH | Brain Injuries/complications | - |
dc.subject.MESH | Brain Injuries/drug therapy* | - |
dc.subject.MESH | Brain Injuries/genetics | - |
dc.subject.MESH | Brain Injuries/pathology | - |
dc.subject.MESH | Brain Ischemia/complications | - |
dc.subject.MESH | Brain Ischemia/drug therapy* | - |
dc.subject.MESH | Brain Ischemia/genetics | - |
dc.subject.MESH | Brain Ischemia/pathology | - |
dc.subject.MESH | Culture Media, Conditioned | - |
dc.subject.MESH | Cyclooxygenase 2/genetics | - |
dc.subject.MESH | Disease Models, Animal | - |
dc.subject.MESH | Endothelial Cells/drug effects | - |
dc.subject.MESH | Endothelial Cells/pathology | - |
dc.subject.MESH | Glucose/metabolism | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Intracranial Hemorrhages/chemically induced | - |
dc.subject.MESH | Intracranial Hemorrhages/drug therapy | - |
dc.subject.MESH | Intracranial Hemorrhages/genetics | - |
dc.subject.MESH | Intracranial Hemorrhages/pathology | - |
dc.subject.MESH | Isoflurane/administration & dosage* | - |
dc.subject.MESH | Matrix Metalloproteinase 2/genetics | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | NF-kappa B/genetics | - |
dc.subject.MESH | Neurons/drug effects | - |
dc.subject.MESH | Neurons/pathology | - |
dc.subject.MESH | Oxygen/metabolism | - |
dc.subject.MESH | Receptors, LDL/genetics | - |
dc.subject.MESH | Stroke/drug therapy* | - |
dc.subject.MESH | Stroke/genetics | - |
dc.subject.MESH | Stroke/pathology | - |
dc.subject.MESH | Tissue Plasminogen Activator/administration & dosage* | - |
dc.subject.MESH | Tissue Plasminogen Activator/adverse effects | - |
dc.subject.MESH | Tissue Plasminogen Activator/genetics | - |
dc.subject.MESH | Tumor Suppressor Proteins/genetics | - |
dc.title | Isoflurane preconditioning inhibits the effects of tissue-type plasminogen activator on brain endothelial cell in an in vitro model of ischemic stroke | - |
dc.type | Article | - |
dc.publisher.location | Australia | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Anesthesiology and Pain Medicine | - |
dc.contributor.googleauthor | So Yeong Cheon | - |
dc.contributor.googleauthor | So Yeon Kim | - |
dc.contributor.googleauthor | Eun Hee Kam | - |
dc.contributor.googleauthor | Jae Hoon Lee | - |
dc.contributor.googleauthor | Jeong Min Kim | - |
dc.contributor.googleauthor | Eun Jung Kim | - |
dc.contributor.googleauthor | Tae Whan Kim | - |
dc.contributor.googleauthor | Bon-Nyeo Koo | - |
dc.identifier.doi | 10.7150/ijms.18037 | - |
dc.contributor.localId | A00616 | - |
dc.contributor.localId | A00816 | - |
dc.contributor.localId | A00884 | - |
dc.contributor.localId | A03092 | - |
dc.contributor.localId | A00193 | - |
dc.relation.journalcode | J02917 | - |
dc.identifier.eissn | 1449-1907 | - |
dc.identifier.pmid | 28539818 | - |
dc.subject.keyword | Tissue-type plasminogen activator | - |
dc.subject.keyword | endothelial cell | - |
dc.subject.keyword | isoflurane | - |
dc.subject.keyword | matrix metalloproteinase | - |
dc.subject.keyword | neuronal cell | - |
dc.subject.keyword | oxygen/glucose deprivation | - |
dc.contributor.alternativeName | Ku, Bon Nyo | - |
dc.contributor.alternativeName | Kim, So Yeon | - |
dc.contributor.alternativeName | Kim, Eun Jung | - |
dc.contributor.alternativeName | Kim, Jeongmin | - |
dc.contributor.alternativeName | Lee, Jae Hoon | - |
dc.contributor.affiliatedAuthor | Kim, So Yeon | - |
dc.contributor.affiliatedAuthor | Kim, Eun Jung | - |
dc.contributor.affiliatedAuthor | Kim, Jeongmin | - |
dc.contributor.affiliatedAuthor | Lee, Jae Hoon | - |
dc.contributor.affiliatedAuthor | Ku, Bon Nyo | - |
dc.citation.title | International Journal of Medical Sciences | - |
dc.citation.volume | 14 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 425 | - |
dc.citation.endPage | 433 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MEDICAL SCIENCES, Vol.14(5) : 425-433, 2017 | - |
dc.date.modified | 2017-11-01 | - |
dc.identifier.rimsid | 42931 | - |
dc.type.rims | ART | - |
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