0 870

Cited 3 times in

ABCB1 2677G>T/A variant enhances chemosensitivity to anti-cancer agents acting on microtubule dynamics through LAMP1 inhibition

DC Field Value Language
dc.contributor.author김태수-
dc.contributor.author라선영-
dc.contributor.author안중배-
dc.contributor.author정현철-
dc.contributor.author정희철-
dc.contributor.author권우선-
dc.date.accessioned2017-11-02T08:08:59Z-
dc.date.available2017-11-02T08:08:59Z-
dc.date.issued2017-
dc.identifier.issn0006-2952-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/154134-
dc.description.abstractOverexpression of ABCB1 associated with single nucleotide variants in cancers was reported to encode a protein responsible for drug resistance. We studied chemosensitivity-related genes associated with ABCB1 2677G>T/A variant. The associated genes were identified based on the results of the significance analysis of microarray, and then prediction accuracy was evaluated using the prediction analysis of microarray. Functional assay of the selected gene was performed by using siRNA and drug accumulation study. A higher frequency of chemoresistance to microtubule-modulating agents was found in cell lines with wild-type ABCB1 compared to cell lines with 2677G>T/A ABCB1 variant. Based on the pharmacogenetic association study with 2677 variant, we identified seven genes that could predict chemosensitivity to microtubule dynamics modulators. The classification accuracy with these seven genes was 90.0%, and the predicted probability was 0.73. LAMP1 was the only gene that was commonly related to chemosensitivity. LAMP1 expression levels were relatively higher in chemoresistant ABCB1 wild-type compared to chemosensitive polymorphic cells. But, there was no difference in ABCB1 expression levels between the two groups. Following LAMP1 siRNA, chemosensitivity was restored due to increased intracellular drug accumulation in wild type cell line. In conclusion, ABCB1 2677G>T/A variant enhances chemosensitivity on microtubule dynamics through LAMP1 inhibition.-
dc.description.statementOfResponsibilityrestriction-
dc.publisherElsevier Science-
dc.relation.isPartOfBIOCHEMICAL PHARMACOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHATP Binding Cassette Transporter, Sub-Family B/genetics-
dc.subject.MESHATP Binding Cassette Transporter, Sub-Family B/physiology-
dc.subject.MESHAntineoplastic Agents/pharmacology*-
dc.subject.MESHCell Line, Tumor-
dc.subject.MESHGene Knockdown Techniques-
dc.subject.MESHHumans-
dc.subject.MESHLysosome-Associated Membrane Glycoproteins/antagonists & inhibitors*-
dc.subject.MESHLysosome-Associated Membrane Glycoproteins/genetics-
dc.subject.MESHMicrotubules/metabolism*-
dc.subject.MESHPharmacogenetics-
dc.subject.MESHRNA, Small Interfering/genetics-
dc.titleABCB1 2677G>T/A variant enhances chemosensitivity to anti-cancer agents acting on microtubule dynamics through LAMP1 inhibition-
dc.typeArticle-
dc.publisher.locationEngland-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Internal Medicine-
dc.contributor.googleauthorWoo Sun Kwon-
dc.contributor.googleauthorSun Young Rha-
dc.contributor.googleauthorHei-Cheul Jeung-
dc.contributor.googleauthorJoong Bae Ahn-
dc.contributor.googleauthorJae-Joon Jung-
dc.contributor.googleauthorDong Hyuk Ki-
dc.contributor.googleauthorTae Soo Kim-
dc.contributor.googleauthorHyun Cheol Chung-
dc.identifier.doi10.1016/j.bcp.2016.11.005-
dc.contributor.localIdA01316-
dc.contributor.localIdA02262-
dc.contributor.localIdA03773-
dc.contributor.localIdA03794-
dc.contributor.localIdA04549-
dc.relation.journalcodeJ00283-
dc.identifier.eissn1873-2968-
dc.identifier.pmid27832934-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0006295216303926-
dc.subject.keywordABCB1-
dc.subject.keywordChemosensitivity-
dc.subject.keywordLAMP1-
dc.subject.keywordVariant-
dc.contributor.alternativeNameKim, Tae Soo-
dc.contributor.alternativeNameRha, Sun Young-
dc.contributor.alternativeNameAhn, Joong Bae-
dc.contributor.alternativeNameChung, Hyun Cheol-
dc.contributor.alternativeNameJeung, Hei Cheul-
dc.contributor.affiliatedAuthorRha, Sun Young-
dc.contributor.affiliatedAuthorAhn, Joong Bae-
dc.contributor.affiliatedAuthorChung, Hyun Cheol-
dc.contributor.affiliatedAuthorJeung, Hei Cheul-
dc.contributor.affiliatedAuthorKim, Tae Soo-
dc.citation.titleBiochemical Pharmacology-
dc.citation.volume123-
dc.citation.startPage73-
dc.citation.endPage84-
dc.identifier.bibliographicCitationBIOCHEMICAL PHARMACOLOGY, Vol.123 : 73-84, 2017-
dc.date.modified2017-11-01-
dc.identifier.rimsid41633-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.