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Detection of Circulating Tumor Cells in Breast Cancer Patients Using Cytokeratin-19 Real-Time RT-PCR

DC FieldValueLanguage
dc.contributor.author김건민-
dc.contributor.author박세호-
dc.contributor.author박형석-
dc.contributor.author손주혁-
dc.contributor.author김승일-
dc.date.accessioned2017-11-02T08:05:32Z-
dc.date.available2017-11-02T08:05:32Z-
dc.date.issued2017-
dc.identifier.issn0513-5796-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/154065-
dc.description.abstractPURPOSE: The roles of circulating tumor cells (CTCs) as predictive and prognostic factors, as well as key mediators in the metastatic cascade, have been investigated. This study aimed to validate a method to quantify CTCs in peripheral blood using a real-time reverse transcriptase polymerase chain reaction (RT-PCR) assay for cytokeratin (CK)-19 and to evaluate the utility of this assay in detecting CTCs in breast cancer patients. MATERIALS AND METHODS: Real-time monitoring PCR of fluorescently labeled specific hybridization probes for CK-19 mRNA was established. Peripheral blood samples from 30 healthy donors, 69 patients with early breast cancer, 47 patients with locally advanced breast cancer, and 126 patients with metastatic breast cancer were prospectively obtained and analyzed for CTC detection. RESULTS: CK-19 mRNA was not detectable in healthy subjects using the real-time RT-PCR method. The detection rates of CK-19 mRNA in breast cancer patients were 47.8% for early breast cancer (33/69), 46.8% for locally advanced breast cancer (22/47), and 61.1% for metastatic breast cancer (77/129). The detection rate of CK-19-positive CTCs in metastatic disease was slightly higher than early or locally advanced breast cancer; however, the detection rate according to disease burden was not statistically different (p=0.097). The detection rate was higher in patients with pleural metastasis (p=0.045). CTC detection was associated with poor survival (p=0.014). CONCLUSION: A highly specific and sensitive CK-19 mRNA-based method to detect CTCs in peripheral blood in breast cancer patients can be used in further prospective studies to evaluate the predictive and prognostic importance of CTCs.-
dc.description.statementOfResponsibilityopen-
dc.formatapplication/pdf-
dc.languageEnglish-
dc.publisherYonsei University-
dc.relation.isPartOfYONSEI MEDICAL JOURNAL-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHBiomarkers, Tumor/blood*-
dc.subject.MESHBreast Neoplasms/blood-
dc.subject.MESHBreast Neoplasms/pathology*-
dc.subject.MESHFemale-
dc.subject.MESHHumans-
dc.subject.MESHKeratin-19/blood*-
dc.subject.MESHKeratin-19/genetics-
dc.subject.MESHNeoplastic Cells, Circulating*-
dc.subject.MESHPrognosis-
dc.subject.MESHProspective Studies-
dc.subject.MESHRNA, Messenger/blood*-
dc.subject.MESHReal-Time Polymerase Chain Reaction-
dc.subject.MESHReverse Transcriptase Polymerase Chain Reaction*/methods-
dc.titleDetection of Circulating Tumor Cells in Breast Cancer Patients Using Cytokeratin-19 Real-Time RT-PCR-
dc.typeArticle-
dc.publisher.locationKorea (South)-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Internal Medicine-
dc.contributor.googleauthorHyung Seok Park-
dc.contributor.googleauthorHyun Ju Han-
dc.contributor.googleauthorSoohyeon Lee-
dc.contributor.googleauthorGun Min Kim-
dc.contributor.googleauthorSeho Park-
dc.contributor.googleauthorYeon A Choi-
dc.contributor.googleauthorJeong Dong Lee-
dc.contributor.googleauthorGi Moon Kim-
dc.contributor.googleauthorJoohyuk Sohn-
dc.contributor.googleauthorSeung Il Kim-
dc.identifier.doi10.3349/ymj.2017.58.1.19-
dc.contributor.localIdA01524-
dc.contributor.localIdA01753-
dc.contributor.localIdA01995-
dc.contributor.localIdA00658-
dc.contributor.localIdA00287-
dc.relation.journalcodeJ02813-
dc.identifier.eissn1976-2437-
dc.identifier.pmid27873491-
dc.subject.keywordCytokeratin-19 mRNA-
dc.subject.keywordRT-PCR-
dc.subject.keywordcirculating tumor cells-
dc.subject.keywordmetastatic breast cancer-
dc.subject.keywordperipheral blood samples-
dc.contributor.alternativeNameKim, Gun Min-
dc.contributor.alternativeNamePark, Se Ho-
dc.contributor.alternativeNamePark, Hyung Seok-
dc.contributor.alternativeNameSohn, Joo Hyuk-
dc.contributor.alternativeNameKim, Seung Il-
dc.contributor.affiliatedAuthorPark, Se Ho-
dc.contributor.affiliatedAuthorPark, Hyung Seok-
dc.contributor.affiliatedAuthorSohn, Joo Hyuk-
dc.contributor.affiliatedAuthorKim, Seung Il-
dc.contributor.affiliatedAuthorKim, Gun Min-
dc.citation.titleYonsei Medical Journal-
dc.citation.volume58-
dc.citation.number1-
dc.citation.startPage19-
dc.citation.endPage26-
dc.identifier.bibliographicCitationYONSEI MEDICAL JOURNAL, Vol.58(1) : 19-26, 2017-
dc.date.modified2017-11-01-
dc.identifier.rimsid41559-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers

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