The pulsed dye laser (PDL) has been shown to be effective for hypertrophic scars and keloids in dermatology initially. The fundamentals of PDL are based on photoacoustic, photothermal and photochemical effects. Because of noninvasive and physiologic features of PDL, it is recently used to treat vocal cord diseases. In particualr, treating sulcus vocalis with PDL (PDL glottoplasty) is a recently introduced surgical treatment. The sulcus vocalis irradiated with PDL can induce collagen synthesis and natural mucosal healing without adherent tissue injury. Although PDL glottoplasty is beneficial in the treatment of sulcus vocalis, we experienced two cases of secondary sublesional laryngeal cysts after PDL glottoplasty during long-term follow-up periods. There have been no reported cases of complication after PDL treatment for sulcus vocalis until now. Therefore, these two cases seem to be the first complication of PDL application reported in literature.