Cited 44 times in
Optimization of the optical transparency of rodent tissues by modified PACT-based passive clearing
DC Field | Value | Language |
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dc.contributor.author | 조영은 | - |
dc.date.accessioned | 2017-10-26T07:59:05Z | - |
dc.date.available | 2017-10-26T07:59:05Z | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 1226-3613 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/152783 | - |
dc.description.abstract | Recently, a bio-electrochemical technique known as CLARITY was reported for three-dimensional phenotype mapping within transparent tissues, allowing clearer whole-body and organ visualization with CB-perfusion (CUBIC) and leading to the development of whole-body clearing and transparency of intact tissues with the PACT (passive clarity technique) and PARS (perfusion-assisted agent release in situ) methodologies. We evaluated the structure-function relationships in circuits of the whole central nervous system (CNS) and various internal organs using improved methods with optimized passive clarity. Thus, in the present study, we aimed to improve the original PACT procedure and passive clearing protocols for different intact rodent tissues. We determined the optimal conditions for the passive clarity method that allowed the production of a transparent whole CNS by clearing the brain and spinal cord, as well as various organs. We also improved the tissue transparency using mPACT (modified PACT), a method for direct passive clearing, and whole perfusion-based PARS-mPACT, a method for fusion clearing, and we identified the appropriate experimental conditions. These optimized methods can be used for easy and economical high-resolution mapping and phenotyping of normal and pathological elements within intact tissues. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/octet-stream | - |
dc.language | English | - |
dc.publisher | Nature Publishing Group | - |
dc.relation.isPartOf | EXPERIMENTAL AND MOLECULAR MEDICINE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Bone and Bones/anatomy & histology | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Central Nervous System/anatomy & histology | - |
dc.subject.MESH | Embryo, Mammalian/anatomy & histology | - |
dc.subject.MESH | Guinea Pigs | - |
dc.subject.MESH | Imaging, Three-Dimensional/methods* | - |
dc.subject.MESH | Immunohistochemistry/methods* | - |
dc.subject.MESH | Light | - |
dc.subject.MESH | Lipids/isolation & purification* | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Mice, Inbred C57BL | - |
dc.subject.MESH | Microscopy, Confocal/methods* | - |
dc.subject.MESH | Models, Anatomic | - |
dc.subject.MESH | Rats | - |
dc.title | Optimization of the optical transparency of rodent tissues by modified PACT-based passive clearing | - |
dc.type | Article | - |
dc.publisher.location | United States | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Neurosurgery | - |
dc.contributor.googleauthor | Jiwon Woo | - |
dc.contributor.googleauthor | Mirae Lee | - |
dc.contributor.googleauthor | Jeong Min Seo | - |
dc.contributor.googleauthor | Hyo Suk Park | - |
dc.contributor.googleauthor | Yong Eun Cho | - |
dc.identifier.doi | 10.1038/emm.2016.105 | - |
dc.contributor.localId | A03865 | - |
dc.relation.journalcode | J00860 | - |
dc.identifier.eissn | 2092-6413 | - |
dc.identifier.pmid | 27909337 | - |
dc.contributor.alternativeName | Cho, Yong Eun | - |
dc.contributor.affiliatedAuthor | Cho, Yong Eun | - |
dc.citation.volume | 48 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 274 | - |
dc.identifier.bibliographicCitation | EXPERIMENTAL AND MOLECULAR MEDICINE, Vol.48(12) : 274, 2016 | - |
dc.date.modified | 2017-10-24 | - |
dc.identifier.rimsid | 39791 | - |
dc.type.rims | ART | - |
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