Cited 1 times in
A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering
DC Field | Value | Language |
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dc.contributor.author | 송재우 | - |
dc.date.accessioned | 2017-10-26T07:56:24Z | - |
dc.date.available | 2017-10-26T07:56:24Z | - |
dc.date.issued | 2016 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/152718 | - |
dc.description.abstract | Photo-thermal angular light scattering (PT-AS) is a novel optical method for measuring the hemoglobin concentration ([Hb]) of blood samples. On the basis of the intrinsic photothermal response of hemoglobin molecules, the sensor enables high-sensitivity, chemical-free measurement of [Hb]. [Hb] detection capability with a limit of 0.12 g/dl over the range of 0.35 - 17.9 g/dl has been demonstrated previously. The method can be readily implemented using inexpensive consumer electronic devices such as a laser pointer and a webcam. The use of a micro-capillary tube as a blood container also enables the hemoglobin assay with a nanoliter-scale blood volume and a low operating cost. Here, detailed instructions for the PT-AS optical setup and signal processing procedures are presented. Experimental protocols and representative results for blood samples in anemic conditions ([Hb] = 5.3, 7.5, and 9.9 g/dl) are also provided, and the measurements are compared with those from a hematology analyzer. Its simplicity in implementation and operation should enable its wide adoption in clinical laboratories and resource-limited settings. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | MYJoVE Corporation | - |
dc.relation.isPartOf | JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering | - |
dc.type | Article | - |
dc.publisher.location | United States | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Laboratory Medicine | - |
dc.contributor.googleauthor | Uihan Kim | - |
dc.contributor.googleauthor | Jaewoo Song | - |
dc.contributor.googleauthor | Suho Ryu | - |
dc.contributor.googleauthor | Soocheol Kim | - |
dc.contributor.googleauthor | Chulmin Joo | - |
dc.identifier.doi | 10.3791/55006 | - |
dc.contributor.localId | A02054 | - |
dc.relation.journalcode | J03021 | - |
dc.identifier.eissn | 1940-087X | - |
dc.relation.journalsince | 2006 | - |
dc.identifier.pmid | 28060269 | - |
dc.identifier.url | https://www.jove.com/video/55006/a-rapid-chemical-free-hemoglobin-assay-with-photothermal-angular | - |
dc.contributor.alternativeName | Song, Jae Woo | - |
dc.contributor.affiliatedAuthor | Song, Jae Woo | - |
dc.citation.volume | 118 | - |
dc.citation.startPage | 55006 | - |
dc.identifier.bibliographicCitation | JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, Vol.118 : 55006, 2016 | - |
dc.date.modified | 2017-10-24 | - |
dc.identifier.rimsid | 39726 | - |
dc.type.rims | ART | - |
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