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Role of MIZ-1 in AMELX gene expression

Authors
 Hee-Jin Noh  ;  Dong-In Koh  ;  Kon-O. Lee  ;  Bu-Nam Jeon  ;  Min-Kyeong Kim  ;  Malcom L. Snead  ;  Man-Wook Hur 
Citation
 Biochemistry and Biophysics Reports, Vol.8 : 340-345, 2016 
Journal Title
 Biochemistry and Biophysics Reports 
ISSN
 2405-5808 
Issue Date
2016
Abstract
Amelogenin (AMELX) is the main component of the developing tooth enamel matrix and is essential for enamel thickness and structure. However, little is known about its transcriptional regulation. Using gene expression analysis, we found that MIZ-1, a potent transcriptional activator of CDKN1A, is expressed during odontoblastic differentiation of hDPSCs (human dental pulp stem cells), and is essential for odontoblast differentiation and mineralization. We also investigated how MIZ-1 regulates gene expression of AMELX. Oligonucleotide-pull down assays showed that MIZ-1 binds to an MRE (MIZ-1 binding element) of the AMELX proximal promoter region (bp, ?170 to ?25). Combined, our ChIP, transient transcription assays, and promoter mutagenesis revealed that MIZ-1 directly binds to the MRE of the Amelx promoter recruits p300 and induces Amelx gene transcription. Finally, we show that the zinc finger protein MIZ-1 is an essential transcriptional activator of Amelx, which is critical in odontogenesis and matrix mineralization in the developing tooth.
Full Text
http://www.sciencedirect.com/science/article/pii/S2405580816302217
DOI
10.1016/j.bbrep.2016.10.007
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
Yonsei Authors
Koh, Dong In(고동인)
Jeon, Bu Nam(전부남)
Hur, Man Wook(허만욱) ORCID logo https://orcid.org/0000-0002-3416-1334
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/152678
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