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Effects of two calcium silicate cements on cell viability, angiogenic growth factor release and related gene expression in stem cells from the apical papilla

Authors
 O. A. Peters  ;  J. Galicia  ;  A. Arias  ;  M. Tolar  ;  E. Ng  ;  S. J. Shin 
Citation
 INTERNATIONAL ENDODONTIC JOURNAL, Vol.49(12) : 1132-1140, 2016 
Journal Title
INTERNATIONAL ENDODONTIC JOURNAL
ISSN
 0143-2885 
Issue Date
2016
Abstract
AIM: To evaluate the effects of two types of calcium silicate cements on viability, angiogenic growth factor release, and angiogenic and inflammation-related gene expression in human stem cells from the apical papilla (SCAP).

METHODOLOGY: SCAPs were grown for 7 days with either ProRoot mineral trioxide aggregate (MTA) or Biodentine (BD). Cell viability and media concentrations of vascular endothelial growth factor (VEGF/VEGFA) and angiopoietin 1 (ANGPT1) were measured. The expression of genes related to angiogenic potential and inflammatory response was measured by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). One-way and two-way analyses of variance with multiple comparisons Tukey's test were performed (P < 0.05).

RESULTS: Cells in contact with either cement were associated with increased cell viability compared with the no-treatment group at day 1 but there were no differences amongst groups at days 3 and 7. Exposure to either cement significantly increased VEGF concentrations at day 3; however, ANGPT-1 levels decreased significantly compared with the no-treatment group at day 3. Exposure to MTA and BD stimulated expression of VEGFA and FIGF/VEGFD. Furthermore, exposure to both cements significantly decreased the mRNA levels of ANGPT1 and FGF2 relative to the no-treatment group.

CONCLUSIONS: Both MTA and BD stimulated the expression of angiogenic genes and release of VEGF, inducing similar expression patterns; however, they appeared to inhibit the expression of specific genes, including ANGPT1 and FGF2.
Full Text
http://onlinelibrary.wiley.com/doi/10.1111/iej.12571/abstract
DOI
10.1111/iej.12571
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Conservative Dentistry (보존과학교실) > 1. Journal Papers
Yonsei Authors
Shin, Su Jung(신수정) ORCID logo https://orcid.org/0000-0001-5795-3919
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/152620
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