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Application of Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry to Screen the Extended-Spectrum β-Lactamase-Producing ST131 Escherichia coli Strains
DC Field | Value | Language |
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dc.contributor.author | 용동은 | - |
dc.contributor.author | 이경원 | - |
dc.date.accessioned | 2017-10-26T07:45:05Z | - |
dc.date.available | 2017-10-26T07:45:05Z | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 2288-0585 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/152471 | - |
dc.description.abstract | Background : Sequence type 131 (ST131) O25b serogroup Escherichia coli, producing CTX-M type extended-spectrum β-lactamase (ESBL), is a major clone involved in worldwide pandemic spread in both community- and healthcare-associated infections. Recently, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has become a routine tool for the identification of bacteria in many laboratories. This study aimed to assess the performance of MALDI-TOF MS for the screening of ESBL-producing E. coli ST131 in a rapid, inexpensive, and simple way. Methods : A total 26 clinical E. coli, isolated from blood between 2013 and 2014, were used. The characteristics are ST131-O25b ESBL producers (n=6), ST131-O16 ESBL producers (n=4), non-ST131 ESBL producers (n=11), and non-ST131 non-ESBL producers (n=5). Specific biomarker peaks to distinguish the ST131 clonal group from others were investigated by MicroIDSys (ASTA, South Korea) and ASTA Tinkerbell 2.0 software. Results : A peak at 9,713 m/z peak is useful to screen for ST131 E. coli, regardless of serogroup O25 or O16, showing a sensitivity of 100%, specificity of 56.2%, positive predictive value of 58.8%, and negative predictive value of 100% when using a relative intensity cutoff of 15%. Conclusion : We can screen for ST131 E. coli using MicroIDSys (ASTA), MALDI-TOF MS in a rapid, inexpensive, and simple way. However, other confirmatory tests are needed to confirm ST131 E. coli due to the low specificity of this method. | - |
dc.description.statementOfResponsibility | open | - |
dc.publisher | Korean Society of Clinical Microbiology | - |
dc.relation.isPartOf | Annals of Clinical Microbiology | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Application of Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry to Screen the Extended-Spectrum β-Lactamase-Producing ST131 Escherichia coli Strains | - |
dc.title.alternative | 말디토프 질량분석법을 이용한 Extended-Spectrum β-Lactamase 생성 ST131 Escherichia coli의 선별 | - |
dc.type | Article | - |
dc.publisher.location | Korea | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Laboratory Medicine | - |
dc.contributor.googleauthor | Young Ah Kim | - |
dc.contributor.googleauthor | Dongeun Yong | - |
dc.contributor.googleauthor | Yong Ha In | - |
dc.contributor.googleauthor | Hyung Soon Park | - |
dc.contributor.googleauthor | Kyungwon Lee | - |
dc.identifier.doi | 10.5145/ACM.2016.19.3.65 | - |
dc.contributor.localId | A02649 | - |
dc.contributor.localId | A02423 | - |
dc.relation.journalcode | J00156 | - |
dc.relation.journalsince | 2013~ | - |
dc.relation.journalbefore | ~2012 Korean Journal of Clinical Microbiology (대한임상미생물학회지) | - |
dc.subject.keyword | Escherichia coli | - |
dc.subject.keyword | Extended-spectrum βlactamase | - |
dc.subject.keyword | Matrix-assisted laser desorption | - |
dc.subject.keyword | ionization time-of-flight mass spectrometry | - |
dc.subject.keyword | Sequence type 131 | - |
dc.subject.keyword | Serogroup O16 | - |
dc.subject.keyword | Serogroup O25b | - |
dc.contributor.alternativeName | Yong, Dong Eun | - |
dc.contributor.alternativeName | Lee, Kyung Won | - |
dc.contributor.affiliatedAuthor | Lee, Kyung Won | - |
dc.contributor.affiliatedAuthor | Yong, Dong Eun | - |
dc.citation.volume | 19 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 65 | - |
dc.citation.endPage | 69 | - |
dc.identifier.bibliographicCitation | Annals of Clinical Microbiology, Vol.19(3) : 65-69, 2016 | - |
dc.date.modified | 2017-10-24 | - |
dc.identifier.rimsid | 48674 | - |
dc.type.rims | ART | - |
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