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국내 한 대학병원의 임상검체에서 분리된 Bacteroides fragilis 독소 유전자의 특성

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dc.contributor.author이경원-
dc.contributor.author임정훈-
dc.date.accessioned2017-10-26T07:44:02Z-
dc.date.available2017-10-26T07:44:02Z-
dc.date.issued2016-
dc.identifier.issn1738-3544-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/152441-
dc.description.abstractEnterotoxigenic Bacteroides fragilis (ETBF) produces enterotoxins known to be a virulence factor. Three isotypes of the B. fragilis toxin (BFT) gene have been identified: bft-1, bft-2, and bft-3. We investigated the presence of bft isotypes in clinical B. fragilis isolates and the antimicrobial resistance of BFT-negative and BFT-positive isolates. Overall, 537 B. fragilis isolates were collected from extraintestinal specimens over 8 years (2006∼2013) from a university hospital in Korea. Samples were analyzed by multiplex PCR to identify the bft gene isotypes. Additionally, the antimicrobial susceptibility of 107 B. fragilis isolates (74 BFT-negative and 33 BFT-positive) was examined by the CLSI agar dilution method. PCR revealed a total bft gene detection rate of 30%, while 33% and 29% of blood and other extraintestinal isolates contained the gene, respectively. Among ETBF isolates, the most common isotype was bft-1 gene, followed by bft-2 and bft-3 (bft-1 77%, bft-2 14%, bft-3 9%). Resistance rates (%) for BFT-negative and positive isolates differed in response to various antimicrobial agents, with 3%, 5%, 1% and 38% of BFT-negative isolates and 3%, 6%, 3% an 42% of BFT-positive isolates being resistant to piperacillin-tazobactam, cefoxitin, imipenem, and clindamycin, respectively. Interestingly, neither BFT-negative nor positive isolates showed antimicrobial resistance to chloramphenicol and metronidazole. Overall, the proportion of ETBF from blood was similar to that of other extraintestinal sites and the bft-1 gene was the predominant isotype. Higher antimicrobial resistance rates were found in BFT-positive isolates than BFT-negative isolates, but these differences were not statistically significant.-
dc.description.statementOfResponsibilityopen-
dc.languageKorean-
dc.publisher대한임상검사학회-
dc.relation.isPartOfKorean Journal of Clinical Laboratory Science-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.title국내 한 대학병원의 임상검체에서 분리된 Bacteroides fragilis 독소 유전자의 특성-
dc.title.alternativeCharacterization of bft Genes among Enterotoxigenic Bacteroides fragilis Isolates from Extraintestinal Specimens at a University Hospital in Korea-
dc.typeArticle-
dc.publisher.locationKorea-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Laboratory Medicine-
dc.contributor.googleauthor김명숙-
dc.contributor.googleauthor김현수-
dc.contributor.googleauthor지승은-
dc.contributor.googleauthor임정훈-
dc.contributor.googleauthor권선영-
dc.contributor.googleauthor김완희-
dc.contributor.googleauthor이기종-
dc.contributor.googleauthor이경원-
dc.identifier.doi10.15324/kjcls.2016.48.2.82-
dc.contributor.localIdA04654-
dc.contributor.localIdA02649-
dc.relation.journalcodeJ01982-
dc.relation.journalsince2013~-
dc.relation.journalbefore~2012 Korean Journal of Clinical Laboratory Science (대한임상검사학회지)-
dc.subject.keywordAntimicrobial resistance-
dc.subject.keywordBacteroides fragilis toxin-
dc.subject.keywordbft gene isotype-
dc.subject.keywordEnterotoxigenic Bacteroides fragilis-
dc.contributor.alternativeNameLee, Kyung Won-
dc.contributor.alternativeNameRim, John Hoon-
dc.contributor.affiliatedAuthorRim, John Hoon-
dc.contributor.affiliatedAuthorLee, Kyung Won-
dc.citation.volume48-
dc.citation.number2-
dc.citation.startPage82-
dc.citation.endPage87-
dc.identifier.bibliographicCitationKorean Journal of Clinical Laboratory Science, Vol.48(2) : 82-87, 2016-
dc.date.modified2017-10-24-
dc.identifier.rimsid48647-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers

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