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국내 한 대학병원의 임상검체에서 분리된 Bacteroides fragilis 독소 유전자의 특성
DC Field | Value | Language |
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dc.contributor.author | 이경원 | - |
dc.contributor.author | 임정훈 | - |
dc.date.accessioned | 2017-10-26T07:44:02Z | - |
dc.date.available | 2017-10-26T07:44:02Z | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 1738-3544 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/152441 | - |
dc.description.abstract | Enterotoxigenic Bacteroides fragilis (ETBF) produces enterotoxins known to be a virulence factor. Three isotypes of the B. fragilis toxin (BFT) gene have been identified: bft-1, bft-2, and bft-3. We investigated the presence of bft isotypes in clinical B. fragilis isolates and the antimicrobial resistance of BFT-negative and BFT-positive isolates. Overall, 537 B. fragilis isolates were collected from extraintestinal specimens over 8 years (2006∼2013) from a university hospital in Korea. Samples were analyzed by multiplex PCR to identify the bft gene isotypes. Additionally, the antimicrobial susceptibility of 107 B. fragilis isolates (74 BFT-negative and 33 BFT-positive) was examined by the CLSI agar dilution method. PCR revealed a total bft gene detection rate of 30%, while 33% and 29% of blood and other extraintestinal isolates contained the gene, respectively. Among ETBF isolates, the most common isotype was bft-1 gene, followed by bft-2 and bft-3 (bft-1 77%, bft-2 14%, bft-3 9%). Resistance rates (%) for BFT-negative and positive isolates differed in response to various antimicrobial agents, with 3%, 5%, 1% and 38% of BFT-negative isolates and 3%, 6%, 3% an 42% of BFT-positive isolates being resistant to piperacillin-tazobactam, cefoxitin, imipenem, and clindamycin, respectively. Interestingly, neither BFT-negative nor positive isolates showed antimicrobial resistance to chloramphenicol and metronidazole. Overall, the proportion of ETBF from blood was similar to that of other extraintestinal sites and the bft-1 gene was the predominant isotype. Higher antimicrobial resistance rates were found in BFT-positive isolates than BFT-negative isolates, but these differences were not statistically significant. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | Korean | - |
dc.publisher | 대한임상검사학회 | - |
dc.relation.isPartOf | Korean Journal of Clinical Laboratory Science | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | 국내 한 대학병원의 임상검체에서 분리된 Bacteroides fragilis 독소 유전자의 특성 | - |
dc.title.alternative | Characterization of bft Genes among Enterotoxigenic Bacteroides fragilis Isolates from Extraintestinal Specimens at a University Hospital in Korea | - |
dc.type | Article | - |
dc.publisher.location | Korea | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Laboratory Medicine | - |
dc.contributor.googleauthor | 김명숙 | - |
dc.contributor.googleauthor | 김현수 | - |
dc.contributor.googleauthor | 지승은 | - |
dc.contributor.googleauthor | 임정훈 | - |
dc.contributor.googleauthor | 권선영 | - |
dc.contributor.googleauthor | 김완희 | - |
dc.contributor.googleauthor | 이기종 | - |
dc.contributor.googleauthor | 이경원 | - |
dc.identifier.doi | 10.15324/kjcls.2016.48.2.82 | - |
dc.contributor.localId | A04654 | - |
dc.contributor.localId | A02649 | - |
dc.relation.journalcode | J01982 | - |
dc.relation.journalsince | 2013~ | - |
dc.relation.journalbefore | ~2012 Korean Journal of Clinical Laboratory Science (대한임상검사학회지) | - |
dc.subject.keyword | Antimicrobial resistance | - |
dc.subject.keyword | Bacteroides fragilis toxin | - |
dc.subject.keyword | bft gene isotype | - |
dc.subject.keyword | Enterotoxigenic Bacteroides fragilis | - |
dc.contributor.alternativeName | Lee, Kyung Won | - |
dc.contributor.alternativeName | Rim, John Hoon | - |
dc.contributor.affiliatedAuthor | Rim, John Hoon | - |
dc.contributor.affiliatedAuthor | Lee, Kyung Won | - |
dc.citation.volume | 48 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 82 | - |
dc.citation.endPage | 87 | - |
dc.identifier.bibliographicCitation | Korean Journal of Clinical Laboratory Science, Vol.48(2) : 82-87, 2016 | - |
dc.date.modified | 2017-10-24 | - |
dc.identifier.rimsid | 48647 | - |
dc.type.rims | ART | - |
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