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The systemic effects of sclerostin overexpression using ΦC31 integrase in mice

DC FieldValueLanguage
dc.contributor.author강명모-
dc.contributor.author임승길-
dc.date.accessioned2017-10-26T07:42:53Z-
dc.date.available2017-10-26T07:42:53Z-
dc.date.issued2016-
dc.identifier.issn0006-291X-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/152414-
dc.description.abstractSclerostin, encoded by the Sost gene, is mainly produced by osteocytes in bone and antagonizes the Wnt/β-catenin signaling pathway, which is a requisite for bone formation. Currently, human anti-sclerostin antibodies are being tested in phase III clinical trials. In addition, serum sclerostin levels are reported to be associated with bone mineral density and fracture risk in normal individuals; however, the correlation between serum sclerostin and bone mass remains controversial. To study the effects of the continuous exposure of exogenous sclerostin on bone, a ΦC31 integrase system, which has the characteristics of site-specificity and efficiency, was applied for the delivery of the Sost gene in this study. We injected Sost-attB plasmid with or without ΦC31 integrase plasmid into the mouse tail vein using a hydrodynamic-based method. The site-specific integration of the Sost gene into the mouse genome was confirmed by examining a pseudo-attP site on the hepatic genomic DNA. Sclerostin was expressed in the hepatocytes, secreted into the blood flow, and maintained at high concentrations in the mice with both Sost-attB plasmid and ΦC31 integrase plasmid injections, which was observed by serial measurement. Moreover, the mice with long-term high levels of serum sclerostin showed trabecular bone loss on micro-CT analysis. Peripheral B cell populations were not affected. Our results suggested that sclerostin could be expressed in the liver and sustained successfully at high levels in the blood by using the ΦC31 integrase system, leading to trabecular bone loss. These findings may help to further ascertain the effects of sclerostin introduced exogenously on the skeleton.-
dc.description.statementOfResponsibilityrestriction-
dc.publisherElsevier-
dc.relation.isPartOfBiochemical and Biophysical Research Communications-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHBacteriophages/enzymology*-
dc.subject.MESHBacteriophages/genetics-
dc.subject.MESHBone and Bones/metabolism*-
dc.subject.MESHBone and Bones/pathology-
dc.subject.MESHGlycoproteins/blood*-
dc.subject.MESHGlycoproteins/genetics*-
dc.subject.MESHIntegrases/genetics*-
dc.subject.MESHMale-
dc.subject.MESHMice-
dc.subject.MESHMice, Inbred ICR-
dc.subject.MESHOsteoporosis/classification*-
dc.subject.MESHOsteoporosis/pathology-
dc.subject.MESHTransfection/methods-
dc.subject.MESHUp-Regulation/genetics-
dc.titleThe systemic effects of sclerostin overexpression using ΦC31 integrase in mice-
dc.typeArticle-
dc.publisher.locationUnited States-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Life Science-
dc.contributor.googleauthorDongdong Zhang-
dc.contributor.googleauthorBo Mi Park-
dc.contributor.googleauthorMyengmo Kang-
dc.contributor.googleauthorHeeJin Nam-
dc.contributor.googleauthorEun Jin Kim-
dc.contributor.googleauthorChuHyun Bae-
dc.contributor.googleauthorSung Kil Lim-
dc.identifier.doi10.1016/j.bbrc.2016.01.178-
dc.contributor.localIdA03375-
dc.contributor.localIdA04871-
dc.relation.journalcodeJ00281-
dc.identifier.pmid26845353-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0006291X16301784?via%3Dihub-
dc.subject.keywordBone-
dc.subject.keywordSerum sclerostin-
dc.subject.keywordSost-
dc.subject.keywordΦC31 integrase-
dc.contributor.alternativeNameKang, Myengmo-
dc.contributor.alternativeNameLim, Sung Kil-
dc.contributor.affiliatedAuthorLim, Sung Kil-
dc.contributor.affiliatedAuthorKang, Myengmo-
dc.citation.volume472-
dc.citation.number3-
dc.citation.startPage471-
dc.citation.endPage476-
dc.identifier.bibliographicCitationBiochemical and Biophysical Research Communications, Vol.472(3) : 471-476, 2016-
dc.date.modified2017-10-24-
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

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