Cited 25 times in
Combination therapy with polymyxin B and netropsin against clinical isolates of multidrug-resistant Acinetobacter baumannii
DC Field | Value | Language |
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dc.contributor.author | 용동은 | - |
dc.date.accessioned | 2017-10-26T07:39:42Z | - |
dc.date.available | 2017-10-26T07:39:42Z | - |
dc.date.issued | 2016 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/152344 | - |
dc.description.abstract | Polymyxins are last-resort antibiotics for treating infections of Gram-negative bacteria. The recent emergence of polymyxin-resistant bacteria, however, urgently demands clinical optimisation of polymyxin use to minimise further evolution of resistance. In this study we developed a novel combination therapy using minimal concentrations of polymyxin B. After large-scale screening of Streptomyces secondary metabolites, we identified a reliable polymixin synergist and confirmed as netropsin using high-pressure liquid chromatography, nuclear magnetic resonance, and mass spectrometry followed by in vitro assays using various Gram-negative pathogenic bacteria. To evaluate the effectiveness of combining polymixin B and netropsin in vivo, we performed survival analysis on greater wax moth Galleria mellonella infected with colistin-resistant clinical Acinetobacter baumannii isolates as well as Escherichia coli, Shigella flexineri, Salmonella typhimuruim, and Pseudomonas aeruginosa. The survival of infected G. mellonella was significantly higher when treated with polymyxin B and netropsin in combination than when treated with polymyxin B or netropsin alone. We propose a netropsin combination therapy that minimises the use of polymyxin B when treating infections with multidrug resistant Gram-negative bacteria. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.language | English | - |
dc.publisher | Nature Publishing Group | - |
dc.relation.isPartOf | SCIENTIFIC REPORTS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Acinetobacter Infections/drug therapy* | - |
dc.subject.MESH | Acinetobacter Infections/microbiology | - |
dc.subject.MESH | Acinetobacter baumannii/drug effects* | - |
dc.subject.MESH | Acinetobacter baumannii/isolation & purification | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Anti-Bacterial Agents/therapeutic use* | - |
dc.subject.MESH | Drug Resistance, Multiple, Bacterial/physiology | - |
dc.subject.MESH | Drug Synergism | - |
dc.subject.MESH | Drug Therapy, Combination | - |
dc.subject.MESH | Escherichia coli/drug effects | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Microbial Sensitivity Tests | - |
dc.subject.MESH | Models, Animal | - |
dc.subject.MESH | Moths/microbiology* | - |
dc.subject.MESH | Netropsin/therapeutic use* | - |
dc.subject.MESH | Polymyxin B/therapeutic use* | - |
dc.subject.MESH | Pseudomonas aeruginosa/drug effects | - |
dc.subject.MESH | Salmonella typhimurium/drug effects | - |
dc.subject.MESH | Shigella flexneri/drug effects | - |
dc.title | Combination therapy with polymyxin B and netropsin against clinical isolates of multidrug-resistant Acinetobacter baumannii | - |
dc.type | Article | - |
dc.publisher.location | England | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Laboratory Medicine | - |
dc.contributor.googleauthor | Joon-hui Chung | - |
dc.contributor.googleauthor | Abhayprasad Bhat | - |
dc.contributor.googleauthor | Chang-Jin Kim | - |
dc.contributor.googleauthor | Dongeun Yong | - |
dc.contributor.googleauthor | Choong-Min Ryu | - |
dc.identifier.doi | 10.1038/srep28168 | - |
dc.contributor.localId | A02423 | - |
dc.relation.journalcode | J02646 | - |
dc.identifier.eissn | 2045-2322 | - |
dc.identifier.pmid | 27306928 | - |
dc.contributor.alternativeName | Yong, Dong Eun | - |
dc.contributor.affiliatedAuthor | Yong, Dong Eun | - |
dc.citation.volume | 6 | - |
dc.citation.startPage | 28168 | - |
dc.identifier.bibliographicCitation | SCIENTIFIC REPORTS, Vol.6 : 28168, 2016 | - |
dc.date.modified | 2017-10-24 | - |
dc.identifier.rimsid | 48079 | - |
dc.type.rims | ART | - |
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