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Microvesicles from brain-extract-treated mesenchymal stem cells improve neurological functions in a rat model of ischemic stroke

DC FieldValueLanguage
dc.contributor.author김동욱-
dc.date.accessioned2017-10-26T07:34:53Z-
dc.date.available2017-10-26T07:34:53Z-
dc.date.issued2016-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/152218-
dc.description.abstractTransplantation of mesenchymal stem cells (MSCs) was reported to improve functional outcomes in a rat model of ischemic stroke, and subsequent studies suggest that MSC-derived microvesicles (MVs) can replace the beneficial effects of MSCs. Here, we evaluated three different MSC-derived MVs, including MVs from untreated MSCs (MSC-MVs), MVs from MSCs treated with normal rat brain extract (NBE-MSC-MVs), and MVs from MSCs treated with stroke-injured rat brain extract (SBE-MSC-MVs), and tested their effects on ischemic brain injury induced by permanent middle cerebral artery occlusion (pMCAO) in rats. NBE-MSC-MVs and SBE-MSC-MVs had significantly greater efficacy than MSC-MVs for ameliorating ischemic brain injury with improved functional recovery. We found similar profiles of key signalling proteins in NBE-MSC-MVs and SBE-MSC-MVs, which account for their similar therapeutic efficacies. Immunohistochemical analyses suggest that brain-extract-treated MSC-MVs reduce inflammation, enhance angiogenesis, and increase endogenous neurogenesis in the rat brain. We performed mass spectrometry proteomic analyses and found that the total proteomes of brain-extract-treated MSC-MVs are highly enriched for known vesicular proteins. Notably, MSC-MV proteins upregulated by brain extracts tend to be modular for tissue repair pathways. We suggest that MSC-MV proteins stimulated by the brain microenvironment are paracrine effectors that enhance MSC therapy for stroke injury.-
dc.description.statementOfResponsibilityopen-
dc.formatapplication/pdf-
dc.languageEnglish-
dc.publisherNature Publishing Group-
dc.relation.isPartOfSCIENTIFIC REPORTS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHBrain*-
dc.subject.MESHBrain Chemistry-
dc.subject.MESHBrain Ischemia/metabolism-
dc.subject.MESHBrain Ischemia/pathology-
dc.subject.MESHBrain Ischemia/physiopathology-
dc.subject.MESHBrain Ischemia/therapy*-
dc.subject.MESHCell-Derived Microparticles*-
dc.subject.MESHComplex Mixtures/chemistry-
dc.subject.MESHComplex Mixtures/pharmacology*-
dc.subject.MESHDisease Models, Animal-
dc.subject.MESHMale-
dc.subject.MESHMesenchymal Stromal Cells*-
dc.subject.MESHRats-
dc.subject.MESHRats, Sprague-Dawley-
dc.subject.MESHRecovery of Function*-
dc.subject.MESHStroke/pathology-
dc.subject.MESHStroke/physiopathology-
dc.subject.MESHStroke/therapy*-
dc.titleMicrovesicles from brain-extract-treated mesenchymal stem cells improve neurological functions in a rat model of ischemic stroke-
dc.typeArticle-
dc.publisher.locationEngland-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Physiology-
dc.contributor.googleauthorJi Yong Lee-
dc.contributor.googleauthorEiru Kim-
dc.contributor.googleauthorSeong-Mi Choi-
dc.contributor.googleauthorDong-Wook Kim-
dc.contributor.googleauthorKwang Pyo Kim-
dc.contributor.googleauthorInsuk Lee-
dc.contributor.googleauthorHan-Soo Kim-
dc.identifier.doi10.1038/srep33038-
dc.contributor.localIdA00406-
dc.relation.journalcodeJ02646-
dc.identifier.eissn2045-2322-
dc.identifier.pmid27609711-
dc.contributor.alternativeNameKim, Dong Wook-
dc.contributor.affiliatedAuthorKim, Dong Wook-
dc.citation.volume6-
dc.citation.startPage33038-
dc.identifier.bibliographicCitationSCIENTIFIC REPORTS, Vol.6 : 33038, 2016-
dc.date.modified2017-10-24-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Physiology (생리학교실) > 1. Journal Papers

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