Cited 51 times in
Resistance Mechanisms and Clinical Features of Fluconazole-Nonsusceptible Candida tropicalis Isolates Compared with Fluconazole-Less-Susceptible Isolates
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이경원 | - |
dc.date.accessioned | 2017-10-26T07:29:45Z | - |
dc.date.available | 2017-10-26T07:29:45Z | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 0066-4804 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/152105 | - |
dc.description.abstract | We investigated the azole resistance mechanisms and clinical features of fluconazole-nonsusceptible (FNS) isolates of Candida tropicalis recovered from Korean surveillance cultures in comparison with fluconazole-less-susceptible (FLS) isolates. Thirty-five clinical isolates of C. tropicalis, comprising 9 FNS (fluconazole MIC, 4 to 64 μg/ml), 12 FLS (MIC, 1 to 2 μg/ml), and 14 control (MIC, 0.125 to 0.5 μg/ml) isolates, were assessed. CDR1, MDR1, and ERG11 expression was quantified, and the ERG11 and UPC2 genes were sequenced. Clinical features of 16 patients with FNS or FLS bloodstream isolates were analyzed. Both FNS and FLS isolates had >10-fold higher mean expression levels of CDR1, MDR1, and ERG11 genes than control isolates (P values of <0.02 for all). When FNS and FLS isolates were compared, FNS isolates had 3.4-fold higher mean ERG11 expression levels than FLS isolates (P = 0.004), but there were no differences in those of CDR1 or MDR1 Of all 35 isolates, 4 (2 FNS and 2 FLS) and 28 (8 FNS, 11 FLS, and 9 control) isolates exhibited amino acid substitutions in Erg11p and Upc2p, respectively. Both FNS and FLS bloodstream isolates were associated with azole therapeutic failure (3/4 versus 4/7) or uncleared fungemia (4/6 versus 4/10), but FNS isolates were identified more frequently from patients with previous azole exposure (6/6 versus 3/10; P = 0.011) and immunosuppression (6/6 versus 3/10; P = 0.011). These results reveal that the majority of FNS C. tropicalis isolates show overexpression of CDR1, MDR1, and ERG11 genes, and fungemia develops after azole exposure in patients with immunosuppression. | - |
dc.description.statementOfResponsibility | open | - |
dc.publisher | American Society for Microbiology | - |
dc.relation.isPartOf | ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | ATP-Binding Cassette Transporters/genetics | - |
dc.subject.MESH | ATP-Binding Cassette Transporters/metabolism | - |
dc.subject.MESH | ATP-Binding Cassette, Sub-Family B, Member 1/genetics | - |
dc.subject.MESH | ATP-Binding Cassette, Sub-Family B, Member 1/metabolism | - |
dc.subject.MESH | Amino Acid Substitution | - |
dc.subject.MESH | Antifungal Agents/pharmacology | - |
dc.subject.MESH | Candida tropicalis/drug effects | - |
dc.subject.MESH | Candida tropicalis/genetics* | - |
dc.subject.MESH | Candida tropicalis/growth & development | - |
dc.subject.MESH | Candida tropicalis/isolation & purification | - |
dc.subject.MESH | Candidiasis/drug therapy | - |
dc.subject.MESH | Candidiasis/etiology | - |
dc.subject.MESH | Candidiasis/immunology | - |
dc.subject.MESH | Candidiasis/microbiology* | - |
dc.subject.MESH | Cytochrome P-450 Enzyme System/genetics | - |
dc.subject.MESH | Cytochrome P-450 Enzyme System/metabolism | - |
dc.subject.MESH | Drug Resistance, Fungal/genetics* | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Fluconazole/pharmacology | - |
dc.subject.MESH | Fungal Proteins/genetics* | - |
dc.subject.MESH | Fungal Proteins/metabolism | - |
dc.subject.MESH | Fungemia/drug therapy | - |
dc.subject.MESH | Fungemia/etiology | - |
dc.subject.MESH | Fungemia/immunology | - |
dc.subject.MESH | Fungemia/microbiology* | - |
dc.subject.MESH | Gene Expression | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Immunosuppressive Agents/adverse effects | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Microbial Sensitivity Tests | - |
dc.subject.MESH | Mutation* | - |
dc.subject.MESH | Public Health Surveillance | - |
dc.subject.MESH | Republic of Korea | - |
dc.subject.MESH | Sequence Analysis, DNA | - |
dc.subject.MESH | Trans-Activators/genetics | - |
dc.subject.MESH | Trans-Activators/metabolism | - |
dc.title | Resistance Mechanisms and Clinical Features of Fluconazole-Nonsusceptible Candida tropicalis Isolates Compared with Fluconazole-Less-Susceptible Isolates | - |
dc.type | Article | - |
dc.publisher.location | United States | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Laboratory Medicine | - |
dc.contributor.googleauthor | Min Ji Choi | - |
dc.contributor.googleauthor | Eun Jeong Won | - |
dc.contributor.googleauthor | Jong Hee Shin | - |
dc.contributor.googleauthor | Soo Hyun Kim | - |
dc.contributor.googleauthor | Wee-Gyo Lee | - |
dc.contributor.googleauthor | Mi-Na Kim | - |
dc.contributor.googleauthor | Kyungwon Lee | - |
dc.contributor.googleauthor | Myung Geun Shin | - |
dc.contributor.googleauthor | Soon Pal Suh | - |
dc.contributor.googleauthor | Dong Wook Ryang | - |
dc.contributor.googleauthor | Young Jun Im | - |
dc.identifier.doi | 10.1128/AAC.02652-15 | - |
dc.contributor.localId | A02649 | - |
dc.relation.journalcode | J00189 | - |
dc.identifier.eissn | 1098-6596 | - |
dc.identifier.pmid | 27044550 | - |
dc.contributor.alternativeName | Lee, Kyung Won | - |
dc.contributor.affiliatedAuthor | Lee, Kyung Won | - |
dc.citation.volume | 60 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 3653 | - |
dc.citation.endPage | 3661 | - |
dc.identifier.bibliographicCitation | ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Vol.60(6) : 3653-3661, 2016 | - |
dc.date.modified | 2017-10-24 | - |
dc.identifier.rimsid | 46885 | - |
dc.type.rims | ART | - |
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