Cited 5 times in
Antibody to FcεRIα Suppresses Immunoglobulin E Binding to High-Affinity Receptor I in Allergic Inflammation
DC Field | Value | Language |
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dc.contributor.author | 김경원 | - |
dc.contributor.author | 김규언 | - |
dc.contributor.author | 손명현 | - |
dc.contributor.author | 홍정연 | - |
dc.date.accessioned | 2017-10-26T07:27:49Z | - |
dc.date.available | 2017-10-26T07:27:49Z | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 0513-5796 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/152063 | - |
dc.description.abstract | Purpose : High-affinity receptor I (FcεRI) on mast cells and basophils plays a key role in the immunoglobulin E (IgE)-mediated type I hypersensitivity mediated by allergen cross-linking of the specific IgE-FcεRI complex. Thus, prevention of IgE binding to FcεRI on these cells is an effective therapy for allergic disease. We have developed a strategy to disrupt IgE binding to FcεRI using an antibody targeting FcεRIα. Materials and Methods : Fab fragment antibodies, which lack the Fc domain, with high affinity and specificity for FcεRIα and effective inhibitory activity against IgE-FcεRI binding were screened. IgE-induced histamine, β-hexosaminidase and Ca2+ release in basophils were determined by ELISA. A B6.Cg-Fcer1atm1Knt Tg(FCER1A)1Bhk/J mouse model of passive cutaneous anaphylaxis (PCA) was used to examine the inhibitory effect of NPB311 on allergic skin inflammation. Results : NPB311 exhibited high affinity to human FcεRIα (KD=4 nM) and inhibited histamine, β-hexosaminidase and Ca2+ release in a concentration-dependent manner in hFcεRI-expressing cells. In hFcεRIα-expressing mice, dye leakage was higher in the PCA group than in controls, but decreased after NPB311 treatment. NPB311 could form a complex with FcεRIα and inhibit the release of inflammation mediators. Conclusion : Our approach for producing anti-FcεRIα Fab fragment antibody NPB311 may enable clinical application to a therapeutic pathway in IgE/FcεRI-mediated diseases. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.language | English | - |
dc.publisher | Yonsei University | - |
dc.relation.isPartOf | YONSEI MEDICAL JOURNAL | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Allergens | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Antibodies, Monoclonal/metabolism | - |
dc.subject.MESH | Antibodies, Monoclonal/pharmacology* | - |
dc.subject.MESH | Basophils/immunology* | - |
dc.subject.MESH | Basophils/metabolism | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Hypersensitivity/immunology | - |
dc.subject.MESH | Immunoglobulin E/immunology | - |
dc.subject.MESH | Immunoglobulin E/metabolism* | - |
dc.subject.MESH | Immunoglobulin E/physiology | - |
dc.subject.MESH | Immunoglobulin Fab Fragments/metabolism* | - |
dc.subject.MESH | Inflammation/metabolism | - |
dc.subject.MESH | Mast Cells | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Receptors, IgE/immunology* | - |
dc.subject.MESH | Receptors, IgE/metabolism | - |
dc.subject.MESH | Receptors, IgE/physiology | - |
dc.title | Antibody to FcεRIα Suppresses Immunoglobulin E Binding to High-Affinity Receptor I in Allergic Inflammation | - |
dc.type | Article | - |
dc.publisher.location | Korea (South) | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Pediatrics | - |
dc.contributor.googleauthor | Jung Yeon Hong | - |
dc.contributor.googleauthor | Jong-Hwan Bae | - |
dc.contributor.googleauthor | Kyung Eun Lee | - |
dc.contributor.googleauthor | Mina Kim | - |
dc.contributor.googleauthor | Min Hee Kim | - |
dc.contributor.googleauthor | Hyun Jung Kang | - |
dc.contributor.googleauthor | Eun Hye Park | - |
dc.contributor.googleauthor | Kyung Sook Yoo | - |
dc.contributor.googleauthor | Se Kyoo Jeong | - |
dc.contributor.googleauthor | Kyung Won Kim | - |
dc.contributor.googleauthor | Kyu-Earn Kim | - |
dc.contributor.googleauthor | Myung Hyun Sohn | - |
dc.identifier.doi | 10.3349/ymj.2016.57.6.1412 | - |
dc.contributor.localId | A00327 | - |
dc.contributor.localId | A01967 | - |
dc.contributor.localId | A00303 | - |
dc.relation.journalcode | J02813 | - |
dc.identifier.eissn | 1976-2437 | - |
dc.identifier.pmid | 27593869 | - |
dc.subject.keyword | Fab fragment | - |
dc.subject.keyword | Immunoglobulin E (IgE) | - |
dc.subject.keyword | antibody affinity | - |
dc.subject.keyword | high-affinity IgE receptor I (FcεRI) | - |
dc.subject.keyword | passive cutaneous anaphylaxis | - |
dc.contributor.alternativeName | Kim, Kyung Won | - |
dc.contributor.alternativeName | Kim, Kyu Earn | - |
dc.contributor.alternativeName | Son, Myung Hyun | - |
dc.contributor.affiliatedAuthor | Kim, Kyu Earn | - |
dc.contributor.affiliatedAuthor | Son, Myung Hyun | - |
dc.contributor.affiliatedAuthor | Kim, Kyung Won | - |
dc.citation.volume | 57 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 1412 | - |
dc.citation.endPage | 1419 | - |
dc.identifier.bibliographicCitation | YONSEI MEDICAL JOURNAL, Vol.57(6) : 1412-1419, 2016 | - |
dc.date.modified | 2017-10-24 | - |
dc.identifier.rimsid | 46843 | - |
dc.type.rims | ART | - |
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