Cited 18 times in
The paraffin-embedded RNA metric (PERM) for RNA isolated from formalin-fixed, paraffin-embedded tissue
DC Field | Value | Language |
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dc.contributor.author | 조한별 | - |
dc.date.accessioned | 2017-10-26T07:27:32Z | - |
dc.date.available | 2017-10-26T07:27:32Z | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 0736-6205 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/152055 | - |
dc.description.abstract | RNA isolated from formalin-fixed, paraffin-embedded (FFPE) tissue is commonly evaluated in both investigative and diagnostic pathology. However, the quality of the data is directly impacted by RNA quality. The RNA integrity number (RIN), an algorithm based on a combination of electrophoretic features, is widely applied to RNA isolated from paraffin-embedded tissue, but it is a poor indicator of the quality of that RNA. Here we describe the novel paraffin-embedded RNA metric (PERM) for quantifying the quality of RNA from FFPE tissue. The PERM is based on a formula that approximates a weighted area-under-the-curve analysis of an electropherogram of the extracted RNA. Using biochemically degraded RNAs prepared from experimentally fixed mouse kidney specimens, we demonstrate that PERM values correlate with mRNA transcript measurements determined using the QuantiGene system. Furthermore, PERM values correlate with real-time PCR data. Our results demonstrate that the PERM can be used to qualify RNA for different end-point studies and may be a valuable tool for molecular studies using RNA extracted from FFPE tissue. | - |
dc.description.statementOfResponsibility | open | - |
dc.publisher | Informa Healthcare | - |
dc.relation.isPartOf | BIOTECHNIQUES | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Benchmarking | - |
dc.subject.MESH | Electrophoresis/standards | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Fixatives/chemistry | - |
dc.subject.MESH | Formaldehyde/chemistry* | - |
dc.subject.MESH | Kidney/chemistry* | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Inbred BALB C | - |
dc.subject.MESH | Paraffin Embedding/standards* | - |
dc.subject.MESH | Quality Control | - |
dc.subject.MESH | RNA/analysis* | - |
dc.subject.MESH | RNA/genetics* | - |
dc.subject.MESH | Real-Time Polymerase Chain Reaction/standards* | - |
dc.subject.MESH | Tissue Fixation/standards | - |
dc.subject.MESH | United States | - |
dc.title | The paraffin-embedded RNA metric (PERM) for RNA isolated from formalin-fixed, paraffin-embedded tissue | - |
dc.type | Article | - |
dc.publisher.location | England | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Obstetrics & Gynecology | - |
dc.contributor.googleauthor | Joon-Yong Chung | - |
dc.contributor.googleauthor | Hanbyoul Cho | - |
dc.contributor.googleauthor | Stephen M. Hewitt | - |
dc.identifier.doi | 10.2144/000114415 | - |
dc.contributor.localId | A03921 | - |
dc.relation.journalcode | J00332 | - |
dc.identifier.eissn | 1940-9818 | - |
dc.identifier.pmid | 27177816 | - |
dc.subject.keyword | RNA integrity | - |
dc.subject.keyword | formalin-fixed | - |
dc.subject.keyword | molecular pathology | - |
dc.subject.keyword | paraffin-embedded | - |
dc.contributor.alternativeName | Cho, Han Byoul | - |
dc.contributor.affiliatedAuthor | Cho, Han Byoul | - |
dc.citation.volume | 60 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 239 | - |
dc.citation.endPage | 244 | - |
dc.identifier.bibliographicCitation | BIOTECHNIQUES, Vol.60(5) : 239-244, 2016 | - |
dc.date.modified | 2017-10-24 | - |
dc.identifier.rimsid | 46835 | - |
dc.type.rims | ART | - |
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