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A polyethylene glycol-based hydrogel as macroporous scaffold for tumorsphere formation of glioblastoma multiforme

Authors
 Yoonjee Oh  ;  Junghwa Cha  ;  Seok-Gu Kang  ;  Pilnam Kim 
Citation
 Journal of Industrial and Engineering Chemistry, Vol.39 : 10-15, 2016 
Journal Title
 Journal of Industrial and Engineering Chemistry 
ISSN
 1226-086X 
Issue Date
2016
Keywords
Polyethylene glycol ; Macroporous hydrogel ; Tumorsphere ; Glioblastoma multiform
Abstract
We report a formation of macroporous scaffold which is based on polyethylene glycol (PEG)-based alginate (ALG) interpenetrating polymer network (IPN) hydrogel. Using this scaffold, we assess the tumorsphere (TS)-forming ability of glioma cancer stem cells (gCSCs), which subpopulation has been highlighted as a main cause of therapeutic resistance due to self-renewal and potential of differentiation properties. Although there have been numerous methods to study the TSs, however, there is no plausible method to evaluate the formation of single gCSC cell-derived TSs, due to fusion-induced cell aggregation. To provide reliable assessment, the PEGDA hydrogel interpenetrated with ALG was fabricated as macroporous scaffold for TS formation of patient-derived gCSCs. With UV-ionic dual crosslinking process, the pore size of PEGDA-ALG hydrogel is magnified enough to be applied as a macroporous scaffold, providing increased internal voids for TS growth and expansion. As a result, within macroporous scaffold, the multiple number of single gCSC-derived TSs was successfully formed inside the structural voids
Full Text
http://www.sciencedirect.com/science/article/pii/S1226086X16301113
DOI
10.1016/j.jiec.2016.05.012
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers
Yonsei Authors
Kang, Seok Gu(강석구) ORCID logo https://orcid.org/0000-0001-5676-2037
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/151916
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