Cited 6 times in
Disk Carbapenemase Test for the Rapid Detection of KPC-, NDM-, and Other Metallo-β-Lactamase-Producing Gram-Negative Bacilli
DC Field | Value | Language |
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dc.contributor.author | 김현수 | - |
dc.contributor.author | 용동은 | - |
dc.contributor.author | 이경원 | - |
dc.contributor.author | 정석훈 | - |
dc.date.accessioned | 2017-10-26T07:18:31Z | - |
dc.date.available | 2017-10-26T07:18:31Z | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 2234-3806 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/151861 | - |
dc.description.abstract | BACKGROUND: Rapid detection of carbapenemase-producing gram-negative bacilli (GNB) is required for optimal treatment of infected patients. We developed and assessed a new disk carbapenemase test (DCT). METHODS: Paper disks containing 0.3 mg of imipenem and bromothymol blue indicator were developed, and the performance of the DCT were evaluated by using 742 strains of GNB with or without carbapenemases. RESULTS: The paper disks were simple to prepare, and the dried disks were stable at -20°C and at 4°C. The DCT detected 212 of 215 strains (98.6% sensitivity with 95% confidence interval [CI] 96.0-99.5%) of GNB with known class A (KPC and Sme) and class B (NDM, IMP, VIM, and SIM) carbapenemases within 60 min, but failed to detect GES-5 carbapenemase. The DCT also detected all two Escherichia coli isolates with OXA-48, but failed to detect GNB with OXA-232, and other OXA carbapenemases. The DCT showed 100% specificity (95% CI, 99.2-100%) in the test of 448 imipenem-nonsusceptible, but carbapenemase genes not tested, clinical isolates of GNB. CONCLUSIONS: The DCT is simple and can be easily performed, even in small laboratories, for the rapid detection of GNB with KPC, NDM and the majority of IMP, VIM, and SIM carbapenemases. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Korean Society for Laboratory Medicine | - |
dc.relation.isPartOf | ANNALS OF LABORATORY MEDICINE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Anti-Bacterial Agents/pharmacology* | - |
dc.subject.MESH | Bacterial Proteins/metabolism* | - |
dc.subject.MESH | Bromthymol Blue/chemistry | - |
dc.subject.MESH | Drug Resistance, Bacterial | - |
dc.subject.MESH | Gram-Negative Bacteria/drug effects | - |
dc.subject.MESH | Gram-Negative Bacteria/enzymology* | - |
dc.subject.MESH | Imipenem/pharmacology | - |
dc.subject.MESH | Microbial Sensitivity Tests/methods* | - |
dc.subject.MESH | Paper | - |
dc.subject.MESH | beta-Lactamases/metabolism* | - |
dc.title | Disk Carbapenemase Test for the Rapid Detection of KPC-, NDM-, and Other Metallo-β-Lactamase-Producing Gram-Negative Bacilli | - |
dc.type | Article | - |
dc.publisher.location | Korea | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Laboratory Medicine | - |
dc.contributor.googleauthor | Hyunsoo Kim | - |
dc.contributor.googleauthor | Ji Yeon Sung | - |
dc.contributor.googleauthor | Dongeun Yong | - |
dc.contributor.googleauthor | Seok Hoon Jeong | - |
dc.contributor.googleauthor | Wonkeun Song | - |
dc.contributor.googleauthor | Kyungwon Lee | - |
dc.contributor.googleauthor | Yunsop Chong | - |
dc.identifier.doi | 10.3343/alm.2016.36.5.434 | - |
dc.contributor.localId | A02423 | - |
dc.contributor.localId | A02649 | - |
dc.contributor.localId | A03619 | - |
dc.contributor.localId | A04829 | - |
dc.relation.journalcode | J00164 | - |
dc.identifier.eissn | 2234-3814 | - |
dc.relation.journalsince | 2012~ | - |
dc.identifier.pmid | 27374708 | - |
dc.relation.journalbefore | ~2011 Korean Journal of Laboratory Medicine (대한진단검사의학회지) | - |
dc.subject.keyword | Disk carbapenemase test | - |
dc.subject.keyword | KPC-type carbapenemase | - |
dc.subject.keyword | Metallo-β-lactamase | - |
dc.subject.keyword | NDM-type carbapenemase | - |
dc.contributor.alternativeName | Kim, Hyun Soo | - |
dc.contributor.alternativeName | Yong, Dong Eun | - |
dc.contributor.alternativeName | Lee, Kyung Won | - |
dc.contributor.alternativeName | Jeong, Seok Hoon | - |
dc.contributor.affiliatedAuthor | Yong, Dong Eun | - |
dc.contributor.affiliatedAuthor | Lee, Kyung Won | - |
dc.contributor.affiliatedAuthor | Jeong, Seok Hoon | - |
dc.contributor.affiliatedAuthor | Kim, Hyun Soo | - |
dc.citation.volume | 36 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 434 | - |
dc.citation.endPage | 440 | - |
dc.identifier.bibliographicCitation | ANNALS OF LABORATORY MEDICINE, Vol.36(5) : 434-440, 2016 | - |
dc.date.modified | 2017-10-24 | - |
dc.identifier.rimsid | 46186 | - |
dc.type.rims | ART | - |
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