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Disk Carbapenemase Test for the Rapid Detection of KPC-, NDM-, and Other Metallo-β-Lactamase-Producing Gram-Negative Bacilli

DC Field Value Language
dc.contributor.author김현수-
dc.contributor.author용동은-
dc.contributor.author이경원-
dc.contributor.author정석훈-
dc.date.accessioned2017-10-26T07:18:31Z-
dc.date.available2017-10-26T07:18:31Z-
dc.date.issued2016-
dc.identifier.issn2234-3806-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/151861-
dc.description.abstractBACKGROUND: Rapid detection of carbapenemase-producing gram-negative bacilli (GNB) is required for optimal treatment of infected patients. We developed and assessed a new disk carbapenemase test (DCT). METHODS: Paper disks containing 0.3 mg of imipenem and bromothymol blue indicator were developed, and the performance of the DCT were evaluated by using 742 strains of GNB with or without carbapenemases. RESULTS: The paper disks were simple to prepare, and the dried disks were stable at -20°C and at 4°C. The DCT detected 212 of 215 strains (98.6% sensitivity with 95% confidence interval [CI] 96.0-99.5%) of GNB with known class A (KPC and Sme) and class B (NDM, IMP, VIM, and SIM) carbapenemases within 60 min, but failed to detect GES-5 carbapenemase. The DCT also detected all two Escherichia coli isolates with OXA-48, but failed to detect GNB with OXA-232, and other OXA carbapenemases. The DCT showed 100% specificity (95% CI, 99.2-100%) in the test of 448 imipenem-nonsusceptible, but carbapenemase genes not tested, clinical isolates of GNB. CONCLUSIONS: The DCT is simple and can be easily performed, even in small laboratories, for the rapid detection of GNB with KPC, NDM and the majority of IMP, VIM, and SIM carbapenemases.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherKorean Society for Laboratory Medicine-
dc.relation.isPartOfANNALS OF LABORATORY MEDICINE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnti-Bacterial Agents/pharmacology*-
dc.subject.MESHBacterial Proteins/metabolism*-
dc.subject.MESHBromthymol Blue/chemistry-
dc.subject.MESHDrug Resistance, Bacterial-
dc.subject.MESHGram-Negative Bacteria/drug effects-
dc.subject.MESHGram-Negative Bacteria/enzymology*-
dc.subject.MESHImipenem/pharmacology-
dc.subject.MESHMicrobial Sensitivity Tests/methods*-
dc.subject.MESHPaper-
dc.subject.MESHbeta-Lactamases/metabolism*-
dc.titleDisk Carbapenemase Test for the Rapid Detection of KPC-, NDM-, and Other Metallo-β-Lactamase-Producing Gram-Negative Bacilli-
dc.typeArticle-
dc.publisher.locationKorea-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Laboratory Medicine-
dc.contributor.googleauthorHyunsoo Kim-
dc.contributor.googleauthorJi Yeon Sung-
dc.contributor.googleauthorDongeun Yong-
dc.contributor.googleauthorSeok Hoon Jeong-
dc.contributor.googleauthorWonkeun Song-
dc.contributor.googleauthorKyungwon Lee-
dc.contributor.googleauthorYunsop Chong-
dc.identifier.doi10.3343/alm.2016.36.5.434-
dc.contributor.localIdA02423-
dc.contributor.localIdA02649-
dc.contributor.localIdA03619-
dc.contributor.localIdA04829-
dc.relation.journalcodeJ00164-
dc.identifier.eissn2234-3814-
dc.relation.journalsince2012~-
dc.identifier.pmid27374708-
dc.relation.journalbefore~2011 Korean Journal of Laboratory Medicine (대한진단검사의학회지)-
dc.subject.keywordDisk carbapenemase test-
dc.subject.keywordKPC-type carbapenemase-
dc.subject.keywordMetallo-β-lactamase-
dc.subject.keywordNDM-type carbapenemase-
dc.contributor.alternativeNameKim, Hyun Soo-
dc.contributor.alternativeNameYong, Dong Eun-
dc.contributor.alternativeNameLee, Kyung Won-
dc.contributor.alternativeNameJeong, Seok Hoon-
dc.contributor.affiliatedAuthorYong, Dong Eun-
dc.contributor.affiliatedAuthorLee, Kyung Won-
dc.contributor.affiliatedAuthorJeong, Seok Hoon-
dc.contributor.affiliatedAuthorKim, Hyun Soo-
dc.citation.volume36-
dc.citation.number5-
dc.citation.startPage434-
dc.citation.endPage440-
dc.identifier.bibliographicCitationANNALS OF LABORATORY MEDICINE, Vol.36(5) : 434-440, 2016-
dc.date.modified2017-10-24-
dc.identifier.rimsid46186-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers

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