Cited 10 times in
Calcium influx-mediated translocation of m-calpain induces Ku80 cleavage and enhances the Ku80-related DNA repair pathway
DC Field | Value | Language |
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dc.contributor.author | 김어수 | - |
dc.date.accessioned | 2017-10-26T07:12:57Z | - |
dc.date.available | 2017-10-26T07:12:57Z | - |
dc.date.issued | 2016 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/151746 | - |
dc.description.abstract | Proteomic analysis of ionomycin-treated and untreated mammary epithelial MCF10A cells elucidated differences in Ku80 cleavage. Ku80, a subunit of the Ku protein complex, is an initiator of the non-homologous, end-joining (NHEJ), double-strand breaks (DSBs) repair pathway. The nuclear Ku80 was cleaved in a calcium concentration-dependent manner by m-calpain but not by m-calpain. The cleavage of nuclear Ku80 at its α/β domain was validated by Western blotting analysis using flag-tagged expression vectors of truncated versions of Ku80 and a flag antibody and was confirmed in m-calpain knock-down cells and in vitro cell-free evaluation with recombinant proteins of calpains, Ku70, and Ku80. In addition, the cleaved Ku80 still formed a Ku heterodimer and promoted DNA DSB repair activity. Taken together, these findings indicate that translocated m-calpain enhances the NHEJ pathway through the cleavage of Ku80. Based on the present study, m-calpain in DNA repair pathways might be a novel anticancer drug target, or its mechanism might be a possible route for resistance acquisition of DNA damage-inducing chemotherapeutics. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.language | English | - |
dc.publisher | Impact Journals | - |
dc.relation.isPartOf | ONCOTARGET | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Antineoplastic Agents/pharmacology | - |
dc.subject.MESH | Antineoplastic Agents/therapeutic use | - |
dc.subject.MESH | Calcium/metabolism* | - |
dc.subject.MESH | Calcium Ionophores/pharmacology | - |
dc.subject.MESH | Calpain/genetics | - |
dc.subject.MESH | Calpain/metabolism* | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Cell-Free System/metabolism | - |
dc.subject.MESH | DNA Breaks, Double-Stranded | - |
dc.subject.MESH | DNA End-Joining Repair/physiology* | - |
dc.subject.MESH | Drug Resistance, Neoplasm | - |
dc.subject.MESH | Epithelial Cells/drug effects | - |
dc.subject.MESH | Gene Knockdown Techniques | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Ionomycin/pharmacology | - |
dc.subject.MESH | Ku Autoantigen/metabolism* | - |
dc.subject.MESH | Molecular Targeted Therapy/methods | - |
dc.subject.MESH | Neoplasms/drug therapy | - |
dc.subject.MESH | Protein Binding | - |
dc.subject.MESH | Protein Domains/physiology | - |
dc.subject.MESH | Protein Multimerization/physiology | - |
dc.subject.MESH | Protein Transport/physiology | - |
dc.subject.MESH | Proteolysis | - |
dc.subject.MESH | Proteomics | - |
dc.subject.MESH | RNA Interference | - |
dc.subject.MESH | RNA, Small Interfering | - |
dc.subject.MESH | Recombinant Proteins/genetics | - |
dc.subject.MESH | Recombinant Proteins/metabolism | - |
dc.title | Calcium influx-mediated translocation of m-calpain induces Ku80 cleavage and enhances the Ku80-related DNA repair pathway | - |
dc.type | Article | - |
dc.publisher.location | United States | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Psychiatry | - |
dc.contributor.googleauthor | Kyung Hye Baek | - |
dc.contributor.googleauthor | Han Vit Yu | - |
dc.contributor.googleauthor | Eosu Kim | - |
dc.contributor.googleauthor | Younghwa Na | - |
dc.contributor.googleauthor | Youngjoo Kwon | - |
dc.identifier.doi | 10.18632/oncotarget.8791 | - |
dc.contributor.localId | A00686 | - |
dc.relation.journalcode | J02421 | - |
dc.identifier.eissn | 1949-2553 | - |
dc.identifier.pmid | 27121057 | - |
dc.subject.keyword | DNA damage | - |
dc.subject.keyword | Ku80-related DNA repair | - |
dc.subject.keyword | adriamycin | - |
dc.subject.keyword | m-calpain | - |
dc.contributor.alternativeName | Kim, Eo Su | - |
dc.contributor.affiliatedAuthor | Kim, Eo Su | - |
dc.citation.volume | 7 | - |
dc.citation.number | 21 | - |
dc.citation.startPage | 30831 | - |
dc.citation.endPage | 30844 | - |
dc.identifier.bibliographicCitation | ONCOTARGET, Vol.7(21) : 30831-30844, 2016 | - |
dc.date.modified | 2017-10-24 | - |
dc.identifier.rimsid | 45759 | - |
dc.type.rims | ART | - |
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