Cited 9 times in
Integrated omics-analysis reveals Wnt-mediated NAD+ metabolic reprogramming in cancer stem-like cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 정재호 | - |
dc.contributor.author | 기현정 | - |
dc.date.accessioned | 2017-10-26T07:09:16Z | - |
dc.date.available | 2017-10-26T07:09:16Z | - |
dc.date.issued | 2016 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/151671 | - |
dc.description.abstract | Abnormal tumor cell metabolism is a consequence of alterations in signaling pathways that provide critical selective advantage to cancer cells. However, a systematic characterization of the metabolic and signaling pathways altered in cancer stem-like cells (CSCs) is currently lacking. Using nuclear magnetic resonance and mass spectrometry, we profiled the whole-cell metabolites of a pair of parental (P-231) and stem-like cancer cells (S-231), and then integrated with whole transcriptome profiles. We identified elevated NAAD+ in S-231 along with a coordinated increased expression of genes in Wnt/calcium signaling pathway, reflecting the correlation between metabolic reprogramming and altered signaling pathways. The expression of CD38 and ALP, upstream NAAD+ regulatory enzymes, was oppositely regulated between P- and S-231; high CD38 strongly correlated with NAADP in P-231 while high ALP with NAAD+ levels in S-231. Antagonizing Wnt activity by dnTCF4 transfection reversed the levels of NAAD+ and ALP expression in S-231. Of note, elevated NAAD+ caused a decrease of cytosolic Ca2+ levels preventing calcium-induced apoptosis in nutrient-deprived conditions. Reprograming of NAD+ metabolic pathway instigated by Wnt signaling prevented cytosolic Ca2+ overload thereby inhibiting calcium-induced apoptosis in S-231. These results suggest that "oncometabolites" resulting from cross talk between the deranged core cancer signaling pathway and metabolic network provide a selective advantage to CSCs. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.language | English | - |
dc.publisher | Impact Journals | - |
dc.relation.isPartOf | ONCOTARGET | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | ADP-ribosyl Cyclase 1/metabolism | - |
dc.subject.MESH | Alkaline Phosphatase/metabolism | - |
dc.subject.MESH | Calcium/metabolism | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Cellular Reprogramming* | - |
dc.subject.MESH | Cytosol/metabolism | - |
dc.subject.MESH | Flow Cytometry | - |
dc.subject.MESH | Gene Expression Profiling | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | In Situ Nick-End Labeling | - |
dc.subject.MESH | Magnetic Resonance Spectroscopy | - |
dc.subject.MESH | Mass Spectrometry | - |
dc.subject.MESH | Membrane Glycoproteins/metabolism | - |
dc.subject.MESH | Metabolomics/methods | - |
dc.subject.MESH | NAD/metabolism* | - |
dc.subject.MESH | NADP/analogs & derivatives* | - |
dc.subject.MESH | NADP/metabolism | - |
dc.subject.MESH | Neoplastic Stem Cells/pathology* | - |
dc.subject.MESH | Spheroids, Cellular | - |
dc.subject.MESH | Transcription Factor 4/genetics | - |
dc.subject.MESH | Transfection | - |
dc.subject.MESH | Wnt Signaling Pathway* | - |
dc.title | Integrated omics-analysis reveals Wnt-mediated NAD+ metabolic reprogramming in cancer stem-like cells | - |
dc.type | Article | - |
dc.publisher.location | United States | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Surgery | - |
dc.contributor.googleauthor | Jueun Lee | - |
dc.contributor.googleauthor | Hyun Jung Kee | - |
dc.contributor.googleauthor | Soonki Min | - |
dc.contributor.googleauthor | Ki Cheong Park | - |
dc.contributor.googleauthor | Sunho Park | - |
dc.contributor.googleauthor | Tae Hyun Hwang | - |
dc.contributor.googleauthor | Do Hyun Ryu | - |
dc.contributor.googleauthor | Geum-Sook Hwang | - |
dc.contributor.googleauthor | Jae-Ho Cheong | - |
dc.identifier.doi | 10.18632/oncotarget.10432 | - |
dc.contributor.localId | A03717 | - |
dc.relation.journalcode | J02421 | - |
dc.identifier.eissn | 1949-2553 | - |
dc.identifier.pmid | 27391070 | - |
dc.subject.keyword | Wnt signaling | - |
dc.subject.keyword | calcium signaling | - |
dc.subject.keyword | cancer stem cells | - |
dc.subject.keyword | integrated analysis | - |
dc.subject.keyword | metabolic reprogramming | - |
dc.contributor.alternativeName | Cheong, Jae Ho | - |
dc.contributor.affiliatedAuthor | Cheong, Jae Ho | - |
dc.citation.volume | 7 | - |
dc.citation.number | 30 | - |
dc.citation.startPage | 48562 | - |
dc.citation.endPage | 48576 | - |
dc.identifier.bibliographicCitation | ONCOTARGET, Vol.7(30) : 48562-48576, 2016 | - |
dc.date.modified | 2017-10-24 | - |
dc.identifier.rimsid | 45685 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.