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Anti-Idiotypic Antibody as a Potential Candidate Vaccine for Neisseria meningitidis Serogroup B

 In Ho Park  ;  Ju Ho Youn  ;  In-Hong Choi  ;  Moon H. Nahm  ;  Se Jong Kim  ;  Jeon-Soo Shin 
 INFECTION AND IMMUNITY, Vol.73(10) : 6399-6406, 2005 
Journal Title
Issue Date
Animals ; Antibodies, Anti-Idiotypic/immunology* ; Antibodies, Monoclonal/immunology* ; Bacterial Capsules ; Immunoglobulin Variable Region/immunology ; Meningococcal Infections/prevention & control ; Meningococcal Vaccines/immunology* ; Mice ; Mice, Inbred BALB C ; Molecular Sequence Data ; Neisseria meningitidis, Serogroup B/immunology* ; Polysaccharides, Bacterial/immunology* ; Surface Plasmon Resonance
Sepsis and meningitis caused by Neisseria meningitidis serogroup B (NMGB) are serious diseases in infants and young adults, but no effective vaccine is available. The capsular polysaccharide (PS) of NMGB has poor immunogenicity and a structural similarity to polysialic acid (PSA) on neuronal tissue that may elicit autoantibodies. Using HmenB3, a protective and nonautoreactive monoclonal antibody (MAb) to NMGB capsular PS, we produced an anti-idiotypic MAb, Naid60, which mimics the capsular PS of NMGB. We produced an anti-anti-idiotypic MAb, MoB34, by using the immunogenic site on Naid60 responsible for inducing the anti-NMGB PS antibody response. MoB34 elicited the complement-mediated killing of representative strains of serogroup B meningococci. MoB34 did not bind to CHP-134, a neuroblastoma cell line expressing α(2-8) PSA, or to mouse brain cryosections at a high concentration. Naid60-keyhole limpet hemocyanin immunization inhibited the growth of live NMGB in intraperitoneally challenged mice; in contrast, three of five control mice developed bacteremia. Thus, Naid60 has an immunogenic site that elicits antibodies with bactericidal activity against NMGB and no autoimmunity to PSA. We suggest that the immunogenic region of Naid60 is a candidate for the development of a new vaccine against NMGB.
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1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Se Jong(김세종)
Park, Inho(박인호) ORCID logo https://orcid.org/0000-0003-2190-5469
Shin, Jeon Soo(신전수) ORCID logo https://orcid.org/0000-0002-8294-3234
Choi, In Hong(최인홍) ORCID logo https://orcid.org/0000-0001-9851-0137
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