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Evaluation of the extraction method for the cytotoxicity testing of latex gloves

Authors
 Hyun Sook Baek  ;  Ja Young Yoo  ;  Dong Kyun Rah  ;  Dong-Wook Han  ;  Dong Hee Lee  ;  Oh-Hun Kwon  ;  Jong-Chul Park 
Citation
 YONSEI MEDICAL JOURNAL, Vol.46(4) : 579-583, 2005 
Journal Title
 YONSEI MEDICAL JOURNAL 
ISSN
 0513-5796 
Issue Date
2005
MeSH
Animals ; Cell Survival/drug effects* ; Cells, Cultured ; Culture Media ; Gloves, Protective ; Latex/isolation & purification* ; Latex/toxicity* ; Mice ; Temperature ; Toxicity Tests/methods*
Keywords
Extraction conditions ; cytotoxicity ; latex gloves ; L-929 cells ; medical devices
Abstract
In this study, the cytotoxicity of medical latex gloves to cultured L-929 cells was determined using various extraction conditions. According to the extraction time and temperature, three types of extraction conditions were used: 1) 24 h at 37℃; 2) 72 h at 37℃; 3) 72 h at 50℃. Also, four different extraction vehicles were used, namely, distilled water (DW), 9 g/l sodium chloride (saline) in DW, and culture media with or without serum. Under the above-mentioned conditions, the samples were extracted and then 2-fold serially diluted in the concentration range 3.13 - 50%. When extracted with either DW or saline for 24 h or 72 h at 37℃, only 50% diluted samples showed distinct cytotoxicity to L-929 cells. Moreover, no cytotoxic potentials were observed when gloves were extracted with DW or saline at 50℃ for 72 h. Cytotoxicity was markedly greater when gloves were extracted with culture medium, irrespective of the presence of serum in the medium. These results suggest that optimal extraction conditions should be established for the cytotoxicity evaluations of biomaterials and medical devices.
Files in This Item:
T200500898.pdf Download
DOI
10.3349/ymj.2005.46.4.579
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Plastic and Reconstructive Surgery (성형외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
Yonsei Authors
Kwon, Oh Hun(권오헌)
Rah, Dong Kyun(나동균)
Park, Jong Chul(박종철) ORCID logo https://orcid.org/0000-0003-0083-5991
Baek, Hyun Sook(백현숙)
Han, Dong Wook(한동욱)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/150785
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