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Glucose-stimulated upregulation of GLUT2 gene is mediated by sterol response element-binding protein-1c in the hepatocytes

DC FieldValueLanguage
dc.contributor.author김경섭-
dc.contributor.author김소연-
dc.contributor.author김재우-
dc.contributor.author김하일-
dc.contributor.author안용호-
dc.contributor.author임승순-
dc.date.accessioned2017-10-26T05:55:09Z-
dc.date.available2017-10-26T05:55:09Z-
dc.date.issued2005-
dc.identifier.issn0012-1797-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/150441-
dc.description.abstractGLUT2 is mainly expressed in the liver, β-cells of the pancreas, and the basolateral membrane of kidney proximal tubules and plays an important role in glucose homeostasis in living organisms. The transcription of the GLUT2 gene is known to be upregulated in the liver during postprandial hyperglycemic states or in type 2 diabetes. However, a molecular mechanism by which glucose activates GLUT2 gene expression is not known. In this study, we report evidence that sterol response element–binding protein (SREBP)-1c plays a key role in glucose-stimulated GLUT2 gene expression. The GLUT2 promoter reporter is activated by SREBP-1c, and the activation is inhibited by a dominant-negative form of SREBP-1c (SREBP-1c DN). Adenoviral expression of SREBP-1c DN suppressed glucose-stimulated GLUT2 mRNA level in primary hepatocytes. An electrophoretic mobility shift assay and mutational analysis of the GLUT2 promoter revealed that SREBP-1c binds to the −84/−76 region of the GLUT2 promoter. Chromatin immunoprecipitation revealed that the binding of SREBP-1c to the −84/−76 region was increased by glucose concentration in a dose-dependent manner. These results indicate that SREBP-1c mediates glucose-stimulated GLUT2 gene expression in hepatocytes.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherAmerican Diabetes Association-
dc.relation.isPartOfDIABETES-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleGlucose-stimulated upregulation of GLUT2 gene is mediated by sterol response element-binding protein-1c in the hepatocytes-
dc.typeArticle-
dc.publisher.locationUnited States-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Biochemistry and Molecular Biology (생화학-분자생물학교실)-
dc.contributor.departmentDept. of Biochemistry and Molecular Biology (생화학-분자생물학교실)-
dc.contributor.departmentDept. of Biochemistry and Molecular Biology (생화학-분자생물학교실)-
dc.contributor.departmentDept. of Biochemistry and Molecular Biology (생화학-분자생물학교실)-
dc.contributor.departmentDept. of Biochemistry and Molecular Biology (생화학-분자생물학교실)-
dc.contributor.departmentDept. of Biochemistry and Molecular Biology (생화학-분자생물학교실)-
dc.contributor.googleauthorSeung-Soon Im-
dc.contributor.googleauthorSeung-Youn Kang-
dc.contributor.googleauthorSo-Youn Kim-
dc.contributor.googleauthorHa-il Kim-
dc.contributor.googleauthorJae-Woo Kim-
dc.contributor.googleauthorKyung-Sup Kim-
dc.contributor.googleauthorYong-Ho Ahn-
dc.identifier.doi10.2337/diabetes.54.6.1684-
dc.contributor.localIdA00297-
dc.contributor.localIdA00617-
dc.contributor.localIdA00865-
dc.contributor.localIdA01092-
dc.contributor.localIdA02249-
dc.contributor.localIdA03376-
dc.relation.journalcodeJ00718-
dc.identifier.eissn1939-327X-
dc.identifier.pmid10.2337/diabetes.54.6.1684-
dc.contributor.alternativeNameKim, Kyung Sup-
dc.contributor.alternativeNameKim, So Youn-
dc.contributor.alternativeNameKim, Jae Woo-
dc.contributor.alternativeNameKim, Ha Il-
dc.contributor.alternativeNameAhn, Yong Ho-
dc.contributor.alternativeNameIm, Seung Soon-
dc.citation.volume54-
dc.citation.number6-
dc.citation.startPage1684-
dc.citation.endPage1691-
dc.identifier.bibliographicCitationDIABETES, Vol.54(6) : 1684-1691, 2005-
dc.date.modified2017-05-04-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers

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