Cited 5 times in
Dose-linear pharmacokinetics, tissue distribution, and excretion of a recombinant fusion protein 125I-GST-TatdMt possessing potent anti-obesity activity
DC Field | Value | Language |
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dc.contributor.author | 허만욱 | - |
dc.date.accessioned | 2017-09-30T06:33:29Z | - |
dc.date.available | 2017-09-30T06:33:29Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 0167-0115 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/150032 | - |
dc.description.abstract | This study first examined the pharmacokinetic disposition of GST-TatdMt, a recombinant Tat protein possessing potent anti-obesity activity, in rats after i.v. injection. GST-TatdMt was over-expressed in E. coli, purified, and radioiodinated using the IODO-GEN method. The radioiodinated 125I-GST-TatdMt was administered to rats at doses of 9 μg (1640 nCi), 18 μg (3388 nCi), and 35 μg (6420 nCi). Upon administration, the total radioactivity in serum declined bi-exponentially, with the average terminal elimination half-life ranging from 13.7 to 15.7 h. There was a linear relationship between dose and AUCINF (r2=1.000) and between dose and Co (r2=0.999). The fraction of administered radioactivity excreted in feces was low (mean range 1.5–2.8%), while the majority of the radioactivity was excreted in urine (mean range 54.9–61.4%). The radioactivity found in the liver, lungs, spleen, and kidneys were higher than in serum, but the tissue-to-serum ratios were relatively low (<1.64). The radioactivity in testes, adipose tissue, heart, and brain was lower than in serum (tissue-to-serum ratios 0.046–0.27). The findings of this study indicate dose-linear pharmacokinetics of 125I-GST-TatdMt in rats over the i.v. dose range studied. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Elsevier/north Holland | - |
dc.relation.isPartOf | REGULATORY PEPTIDES | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Gene Products, tat/administration & dosage | - |
dc.subject.MESH | Gene Products, tat/genetics | - |
dc.subject.MESH | Gene Products, tat/pharmacokinetics* | - |
dc.subject.MESH | Glutathione Transferase/administration & dosage | - |
dc.subject.MESH | Glutathione Transferase/genetics | - |
dc.subject.MESH | Glutathione Transferase/pharmacokinetics* | - |
dc.subject.MESH | Injections, Intravenous | - |
dc.subject.MESH | Iodine Radioisotopes/administration & dosage | - |
dc.subject.MESH | Iodine Radioisotopes/pharmacokinetics | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Obesity/drug therapy* | - |
dc.subject.MESH | Obesity/metabolism | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Rats, Sprague-Dawley | - |
dc.subject.MESH | Recombinant Fusion Proteins/administration & dosage | - |
dc.subject.MESH | Recombinant Fusion Proteins/genetics | - |
dc.subject.MESH | Recombinant Fusion Proteins/pharmacokinetics* | - |
dc.title | Dose-linear pharmacokinetics, tissue distribution, and excretion of a recombinant fusion protein 125I-GST-TatdMt possessing potent anti-obesity activity | - |
dc.type | Article | - |
dc.publisher.location | Netherlands | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) | - |
dc.contributor.googleauthor | Beom Soo Shin | - |
dc.contributor.googleauthor | Chul Hwan Kim | - |
dc.contributor.googleauthor | Yun Sik Jun | - |
dc.contributor.googleauthor | Eun Hye Park | - |
dc.contributor.googleauthor | Man-Wook Hur | - |
dc.contributor.googleauthor | Kang Choon Lee | - |
dc.contributor.googleauthor | Yu Seok Yoon | - |
dc.contributor.googleauthor | Min-Nyung Lee | - |
dc.contributor.googleauthor | Sun Dong Yoo | - |
dc.identifier.doi | 10.1016/j.regpep.2005.01.010 | - |
dc.contributor.localId | A04350 | - |
dc.relation.journalcode | J02602 | - |
dc.identifier.eissn | 1873-1686 | - |
dc.identifier.pmid | 15927694 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0167011505000224 | - |
dc.subject.keyword | Tat | - |
dc.subject.keyword | GST-TatdMt | - |
dc.subject.keyword | Obesity | - |
dc.subject.keyword | Pharmacokinetics | - |
dc.subject.keyword | Tissue distribution | - |
dc.contributor.alternativeName | Hur, Man Wook | - |
dc.citation.volume | 129 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 25 | - |
dc.citation.endPage | 30 | - |
dc.identifier.bibliographicCitation | REGULATORY PEPTIDES, Vol.129(1) : 25-30, 2005 | - |
dc.date.modified | 2017-05-04 | - |
dc.identifier.rimsid | 42714 | - |
dc.type.rims | ART | - |
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