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Dose-linear pharmacokinetics, tissue distribution, and excretion of a recombinant fusion protein 125I-GST-TatdMt possessing potent anti-obesity activity

DC FieldValueLanguage
dc.contributor.author허만욱-
dc.date.accessioned2017-09-30T06:33:29Z-
dc.date.available2017-09-30T06:33:29Z-
dc.date.issued2005-
dc.identifier.issn0167-0115-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/150032-
dc.description.abstractThis study first examined the pharmacokinetic disposition of GST-TatdMt, a recombinant Tat protein possessing potent anti-obesity activity, in rats after i.v. injection. GST-TatdMt was over-expressed in E. coli, purified, and radioiodinated using the IODO-GEN method. The radioiodinated 125I-GST-TatdMt was administered to rats at doses of 9 μg (1640 nCi), 18 μg (3388 nCi), and 35 μg (6420 nCi). Upon administration, the total radioactivity in serum declined bi-exponentially, with the average terminal elimination half-life ranging from 13.7 to 15.7 h. There was a linear relationship between dose and AUCINF (r2=1.000) and between dose and Co (r2=0.999). The fraction of administered radioactivity excreted in feces was low (mean range 1.5–2.8%), while the majority of the radioactivity was excreted in urine (mean range 54.9–61.4%). The radioactivity found in the liver, lungs, spleen, and kidneys were higher than in serum, but the tissue-to-serum ratios were relatively low (<1.64). The radioactivity in testes, adipose tissue, heart, and brain was lower than in serum (tissue-to-serum ratios 0.046–0.27). The findings of this study indicate dose-linear pharmacokinetics of 125I-GST-TatdMt in rats over the i.v. dose range studied.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherElsevier/north Holland-
dc.relation.isPartOfREGULATORY PEPTIDES-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHGene Products, tat/administration & dosage-
dc.subject.MESHGene Products, tat/genetics-
dc.subject.MESHGene Products, tat/pharmacokinetics*-
dc.subject.MESHGlutathione Transferase/administration & dosage-
dc.subject.MESHGlutathione Transferase/genetics-
dc.subject.MESHGlutathione Transferase/pharmacokinetics*-
dc.subject.MESHInjections, Intravenous-
dc.subject.MESHIodine Radioisotopes/administration & dosage-
dc.subject.MESHIodine Radioisotopes/pharmacokinetics-
dc.subject.MESHMale-
dc.subject.MESHObesity/drug therapy*-
dc.subject.MESHObesity/metabolism-
dc.subject.MESHRats-
dc.subject.MESHRats, Sprague-Dawley-
dc.subject.MESHRecombinant Fusion Proteins/administration & dosage-
dc.subject.MESHRecombinant Fusion Proteins/genetics-
dc.subject.MESHRecombinant Fusion Proteins/pharmacokinetics*-
dc.titleDose-linear pharmacokinetics, tissue distribution, and excretion of a recombinant fusion protein 125I-GST-TatdMt possessing potent anti-obesity activity-
dc.typeArticle-
dc.publisher.locationNetherlands-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Biochemistry and Molecular Biology (생화학-분자생물학교실)-
dc.contributor.googleauthorBeom Soo Shin-
dc.contributor.googleauthorChul Hwan Kim-
dc.contributor.googleauthorYun Sik Jun-
dc.contributor.googleauthorEun Hye Park-
dc.contributor.googleauthorMan-Wook Hur-
dc.contributor.googleauthorKang Choon Lee-
dc.contributor.googleauthorYu Seok Yoon-
dc.contributor.googleauthorMin-Nyung Lee-
dc.contributor.googleauthorSun Dong Yoo-
dc.identifier.doi10.1016/j.regpep.2005.01.010-
dc.contributor.localIdA04350-
dc.relation.journalcodeJ02602-
dc.identifier.eissn1873-1686-
dc.identifier.pmid15927694-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0167011505000224-
dc.subject.keywordTat-
dc.subject.keywordGST-TatdMt-
dc.subject.keywordObesity-
dc.subject.keywordPharmacokinetics-
dc.subject.keywordTissue distribution-
dc.contributor.alternativeNameHur, Man Wook-
dc.citation.volume129-
dc.citation.number1-
dc.citation.startPage25-
dc.citation.endPage30-
dc.identifier.bibliographicCitationREGULATORY PEPTIDES, Vol.129(1) : 25-30, 2005-
dc.date.modified2017-05-04-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers

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