Cited 17 times in
Histone deacetylase-1 represses transcription by interacting with zinc-fingers and interfering with the DNA binding activity of Sp1
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김명화 | - |
dc.contributor.author | 박혜진 | - |
dc.contributor.author | 이정안 | - |
dc.contributor.author | 허만욱 | - |
dc.date.accessioned | 2017-09-30T06:33:11Z | - |
dc.date.available | 2017-09-30T06:33:11Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 1015-8987 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/150027 | - |
dc.description.abstract | Sp1 activates the transcription of many cellular and viral genes, and histone deacetylase 1 (HDAC1) removes the acetyl group of nucleosomal core histones. Treatment of cells with the histone deacetylase 1 inhibitor, TSA, robustly activates the transcription of the Sp1-dependent promoters, suggesting the inhibition of Sp1 activity which is critical in the activation of transcription, by HDAC1. We assessed the protein-protein interactions occurring between Sp1 and HDAC1, and the transcriptional regulatory mechanism controlled by this interaction. In vitro GST fusion pull down assays, co-immunoprecipitation, and mammalian two-hybrid assays revealed that the HDAC1 noncatalytic domain (a.a. 237-482) interacts directly with the zinc-finger DNA binding domain of Sp1. DNase I footprinting revealed that this interaction prevents the binding of Sp1 zinc-fingers to the target GC-box. Gal4-HDAC1 fusion, targeted proximally to the GC-boxes, potently repressed the transcription of pG5-5x(GC)-Luc, in which Sp1 potently activates transcription. This repression of transcription does not involve the deacetylase activity of HDAC1, and is accomplished by the direct protein-protein interactions which occur between the Sp1 zinc-finger DNA binding domain and HDAC1, which interferes with the promoter GC-box binding of Sp1. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | S. Karger | - |
dc.relation.isPartOf | CELLULAR PHYSIOLOGY AND BIOCHEMISTRY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Sp1 | - |
dc.subject.MESH | Histone deacetylase 1 | - |
dc.subject.MESH | GC-box | - |
dc.subject.MESH | Trichostatin A, TSA | - |
dc.subject.MESH | Transcription | - |
dc.subject.MESH | Repression | - |
dc.title | Histone deacetylase-1 represses transcription by interacting with zinc-fingers and interfering with the DNA binding activity of Sp1 | - |
dc.type | Article | - |
dc.publisher.location | Switzerland | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) | - |
dc.contributor.department | Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) | - |
dc.contributor.department | Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) | - |
dc.contributor.department | Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) | - |
dc.contributor.googleauthor | Kang J.-E. | - |
dc.contributor.googleauthor | Kim M.-H. | - |
dc.contributor.googleauthor | Lee J.-A. | - |
dc.contributor.googleauthor | Park H. | - |
dc.contributor.googleauthor | Min-Nyung L. | - |
dc.contributor.googleauthor | Auh C.-K. | - |
dc.contributor.googleauthor | Hur M.-W | - |
dc.identifier.doi | 10.1159/000087728 | - |
dc.contributor.localId | A00430 | - |
dc.contributor.localId | A01770 | - |
dc.contributor.localId | A04862 | - |
dc.contributor.localId | A04350 | - |
dc.relation.journalcode | J00501 | - |
dc.identifier.eissn | 1421-9778 | - |
dc.identifier.pmid | 10.1159/000087728 | - |
dc.identifier.url | http://www.karger.com/Article/FullText/87728 | - |
dc.subject.keyword | Sp1 | - |
dc.subject.keyword | Histone deacetylase 1 | - |
dc.subject.keyword | GC-box | - |
dc.subject.keyword | Trichostatin A, TSA | - |
dc.subject.keyword | Transcription | - |
dc.subject.keyword | Repression | - |
dc.contributor.alternativeName | Kim, Myung Hwa | - |
dc.contributor.alternativeName | Park, Hye Jin | - |
dc.contributor.alternativeName | Lee, J. A. | - |
dc.contributor.alternativeName | Hur, Man Wook | - |
dc.citation.volume | 16 | - |
dc.citation.number | 1~3 | - |
dc.citation.startPage | 23 | - |
dc.citation.endPage | 30 | - |
dc.identifier.bibliographicCitation | CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, Vol.16(1~3) : 23-30, 2005 | - |
dc.date.modified | 2017-05-04 | - |
dc.identifier.rimsid | 42709 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.