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제대혈 유래 CD34 양성세포로부터 적혈구 분화유도와 microarray를 이용한 유전자 프로파일 분석

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dc.contributor.author김현숙-
dc.contributor.author김현옥-
dc.contributor.author라선영-
dc.contributor.author유철주-
dc.date.accessioned2017-09-29T06:30:50Z-
dc.date.available2017-09-29T06:30:50Z-
dc.date.issued2005-
dc.identifier.issn1598-6535-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/149877-
dc.description.abstractBackground :In this study, we attempted to generate RBCs from CD34+ cells in cord blood using a 3-step culture protocol and also evaluated a change in immunophenotypic characteristics and expression profile according to erythropoietin (EPO) concentrations and culture duration. Method :Using mini-MACS columns, CD34+ cells were isolated from cord blood. The culture procedure comprised three steps. For each step, cells were cultured sequentially for 7 days in a serum free liquid medium with a specific combination of growth factors for 21 days. [1st step: Flt3-ligand (Flt3-L), thrombopoietin and stem cell factor (SCF); 2nd step: IGF-1, SCF and EPO; and 3rd step: IGF-1 and EPO] To evaluate the effect of EPO on proliferation and differentiation, cells were cultured with different EPO concentrations (0, 3, 10 & 20 U/mL). Cell count and morphology were monitored during the culture. For phenotyping, antibodies to CD34, CD38, CD45 and glycophorin A (GPA) were used. The expression profile of cultured cells was analyzed by 17,000-gene microarray analysis. Result :As EPO concentration increased, cell expansion was also increased, showing a maximum expansion at 20 U/mL. The cell population showed a gradual decrease in expression of CD34 and CD45, whereas the expression of GPA was not prominent in any conditions. However, we observed increased expression in some genes associated with erythropoiesis (e.g. glycophorin A, rhesus blood group CcEe antigens). Conclusion :This study shows that erythropoietin enhances the proliferation of hematopoietic progenitor cells. Our culture system did not achieve pure production of RBCs, but induced expression changes that indicated erythroid differentiation.-
dc.description.statementOfResponsibilityopen-
dc.languageKorean-
dc.publisher대한진단검사의학회-
dc.relation.isPartOfKOREAN JOURNAL OF LABORATORY MEDICINE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHCord blood-
dc.subject.MESHCD34+ cells-
dc.subject.MESHErythropoietin-
dc.subject.MESHRBCs-
dc.title제대혈 유래 CD34 양성세포로부터 적혈구 분화유도와 microarray를 이용한 유전자 프로파일 분석-
dc.title.alternativeEx Vivo Generation of RBCs from CD34+ Cells in Human Umbilical Cord Blood and Expression Profile Analysis Using Microarray-
dc.typeArticle-
dc.publisher.locationKorea-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Laboratory Medicine (진단검사의학교실)-
dc.contributor.departmentDept. of Laboratory Medicine (진단검사의학교실)-
dc.contributor.departmentDept. of Internal Medicine (내과학교실)-
dc.contributor.departmentDept. of Pediatrics (소아청소년과학교실)-
dc.contributor.googleauthor김창기-
dc.contributor.googleauthor이지혁-
dc.contributor.googleauthor박광일-
dc.contributor.googleauthor김현숙-
dc.contributor.googleauthor유철주-
dc.contributor.googleauthor라선영-
dc.contributor.googleauthor김현옥-
dc.identifier.doiOAK-2005-06764-
dc.contributor.localIdA01117-
dc.contributor.localIdA01122-
dc.contributor.localIdA01316-
dc.contributor.localIdA02524-
dc.relation.journalcodeJ02045-
dc.relation.journalsince2002~2006-
dc.relation.journalafter2006~ Korean Journal of Laboratory Medicine-
dc.relation.journalbefore~2005 Korean Journal of Clinical Pathology (대한임상병리학회지)-
dc.subject.keywordCord blood-
dc.subject.keywordCD34+ cells-
dc.subject.keywordErythropoietin-
dc.subject.keywordRBCs-
dc.contributor.alternativeNameKim, Hyon Suk-
dc.contributor.alternativeNameKim, Hyun Ok-
dc.contributor.alternativeNameRha, Sun Young-
dc.contributor.alternativeNameLyu, Chuhl Joo-
dc.citation.volume25-
dc.citation.number6-
dc.citation.startPage457-
dc.citation.endPage464-
dc.identifier.bibliographicCitationKOREAN JOURNAL OF LABORATORY MEDICINE , Vol.25(6) : 457-464, 2005-
dc.date.modified2017-05-04-
dc.identifier.rimsid41969-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아과학교실) > 1. Journal Papers

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