Cited 0 times in
Functional Study of O-GlcNAcylation on ULK1 under glucose deprivation
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Pak, Jin Won | - |
dc.date.accessioned | 2017-07-11T16:10:20Z | - |
dc.date.available | 2017-07-11T16:10:20Z | - |
dc.date.issued | 2016 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/149127 | - |
dc.description | Dept. of Integrated OMICS for Biomedical Science,/박사 | - |
dc.description.abstract | O-GlcNAcylation is a dynamic posttranslational modification that occurs in the serine or threonine residues of nuclear and cytosolic proteins. O-GlcNAcylation regulates various cellular events, such as a nutrient sensing, epigenetic regulation, translational regulation, and cell proliferation, as well as disease-related signal pathways. UDP-GlcNAc, a precursor of O-GlcNAcylation, is synthesized from glucose by the hexosamine biosynthetic pathway (HBP). The level of UDP-GlcNAc depends on the cellular glucose condition. For this reason, O-GlcNAcylation is called a nutrient sensor. Although O-GlcNAcylation plays a role in nutrient sensing and metabolism, the relationship between O-GlcNAcylation and autophagy remains unknown. In previous studies, decreased O-GlcNAcylation by OGT siRNA induced autophagy without starvation. In this study, we observed that a unc-51 like autophagy activating kinase 1(ULK1), a regulator of autophagy, is O-GlcNAcylated. Under glucose deprivation, O-GlcNAcylated ULk1 is increased, and increased O-GlcNAcylation induces ULK1 S757 phosphorylation, which leads to ULK1 inactivation. The ULK1 S757A mutant decreases O-GlcNAcylation on ULK1 and binding affinity with OGT compared with ULK1 wild type. These data imply that O-GlcNAcylation on ULK1 decreases ULK1 kinase activity by increasing S757 phosphorylation and reduces autophagy induction. | - |
dc.description.statementOfResponsibility | open | - |
dc.publisher | Graduate School, Yonsei University | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Functional Study of O-GlcNAcylation on ULK1 under glucose deprivation | - |
dc.title.alternative | 포도당 결핍상태에서 ULK1의 O-GlcNAc 수식화 기능연구 | - |
dc.type | Thesis | - |
dc.contributor.alternativeName | Pak, Jin Won | - |
dc.type.local | Dissertation | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.