Cited 127 times in
Caspase-2 primers cancer cells for TRAIL-mediated apoptosis by processing procaspase-8
DC Field | Value | Language |
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dc.contributor.author | 김건홍 | - |
dc.contributor.author | 신순아 | - |
dc.date.accessioned | 2017-05-08T08:01:46Z | - |
dc.date.available | 2017-05-08T08:01:46Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 0261-4189 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/147683 | - |
dc.description.abstract | Although caspase-2 is believed to be involved in death receptor-mediated apoptosis, the exact function, mode of activation, and regulation of caspase-2 remain unknown. Here we show that protein kinase (PK) CK2 phosphorylates procaspase-2 directly at serine-157. When intracellular PKCK2 activity is low or downregulated by specific inhibitors, procaspase-2 is dephosphorylated, dimerized, and activated in a PIDDosome-independent manner. The activated caspase-2 then processes procaspase-8 monomers between the large and small subunits, thereby priming cancer cells for TNF-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis. The processed procaspase-8 that is recruited to death-inducing signaling complex by TRAIL engagement becomes fully activated, and cancer cells undergo apoptosis. PKCK2 activity is low in TRAIL-sensitive cancer cell lines but high in TRAIL-resistant cancer cell lines. Thus, downregulating PKCK2 activity is required for TRAIL-mediated apoptosis to occur in TRAIL-resistant cancer cells. Our data provide novel insights into the regulation, mode of activation, and function of caspase-2 in TRAIL-mediated apoptosis. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 3532~3542 | - |
dc.language | English | - |
dc.publisher | Wiley Blackwell | - |
dc.relation.isPartOf | EMBO JOURNAL | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Apoptosis* | - |
dc.subject.MESH | Apoptosis Regulatory Proteins/pharmacology | - |
dc.subject.MESH | Casein Kinase II/antagonists & inhibitors | - |
dc.subject.MESH | Casein Kinase II/genetics | - |
dc.subject.MESH | Casein Kinase II/metabolism* | - |
dc.subject.MESH | Caspase 2 | - |
dc.subject.MESH | Caspase 8 | - |
dc.subject.MESH | Caspases/genetics | - |
dc.subject.MESH | Caspases/metabolism* | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Tumor | - |
dc.subject.MESH | Dimerization | - |
dc.subject.MESH | Enzyme Activation | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Membrane Glycoproteins/pharmacology | - |
dc.subject.MESH | Neoplasms/enzymology* | - |
dc.subject.MESH | Phosphorylation | - |
dc.subject.MESH | Serine/metabolism | - |
dc.subject.MESH | TNF-Related Apoptosis-Inducing Ligand | - |
dc.subject.MESH | Tumor Necrosis Factor-alpha/pharmacology | - |
dc.title | Caspase-2 primers cancer cells for TRAIL-mediated apoptosis by processing procaspase-8 | - |
dc.type | Article | - |
dc.publisher.location | England | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) | - |
dc.contributor.department | Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) | - |
dc.contributor.googleauthor | Soonah Shin | - |
dc.contributor.googleauthor | Yoonmi Lee | - |
dc.contributor.googleauthor | Wooseok Kim | - |
dc.contributor.googleauthor | Hyeonseok Ko | - |
dc.contributor.googleauthor | Hyeyeon Choi | - |
dc.contributor.googleauthor | Kunhong Kim | - |
dc.identifier.doi | 10.1038/sj.emboj.7600827 | - |
dc.contributor.localId | A00289 | - |
dc.contributor.localId | A02119 | - |
dc.relation.journalcode | J00763 | - |
dc.identifier.eissn | 1460-2075 | - |
dc.identifier.pmid | 16193064 | - |
dc.subject.keyword | caspase-2 | - |
dc.subject.keyword | caspase-8 | - |
dc.subject.keyword | PKCK2 | - |
dc.subject.keyword | priming | - |
dc.subject.keyword | TRAIL | - |
dc.contributor.alternativeName | Kim, Kun Hong | - |
dc.contributor.alternativeName | Shin, Soonah | - |
dc.citation.volume | 24 | - |
dc.citation.number | 20 | - |
dc.citation.startPage | 3532 | - |
dc.citation.endPage | 3542 | - |
dc.identifier.bibliographicCitation | EMBO JOURNAL, Vol.24(20) : 3532-3542, 2005 | - |
dc.date.modified | 2017-05-04 | - |
dc.identifier.rimsid | 45226 | - |
dc.type.rims | ART | - |
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