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Degranulation of human eosinophils induced by Paragonimus westermani-secreted protease

Authors
 Myeong Heon Shin  ;  Young-Bae Chung  ;  Hirohito Kita 
Citation
 KOREAN JOURNAL OF PARASITOLOGY, Vol.43(1) : 1-11, 2005 
Journal Title
 KOREAN JOURNAL OF PARASITOLOGY 
ISSN
 0023-4001 
Issue Date
2005
MeSH
Paragonimus westermani ; excretory-secretory products (ESP) ; cysteine protease ; eosinophils ; degranulation
Keywords
Paragonimus westermani ; excretory-secretory products (ESP) ; cysteine protease ; eosinophils ; degranulation
Abstract
Eosinophil degranulation is considered to be a key effector function for the killing of helminthic worms and tissue inflammation at worm-infected lesion sites. However, relatively little data are available with regard to eosinophil response after stimulation with worm-secreted products which contain a large quantity of cysteine proteases. In this study, we attempted to determine whether the degranulation of human eosinophils could be induced by the direct stimulation of the excretory-secretory products (ESP) of Paragonimus westermani, which causes pulmonary paragonimiasis in human beings. Incubation of eosinophils for 3 hr with Paragonimus-secreted products resulted in marked degranulation, as evidenced by the release of eosinophil-derived neurotoxin (EDN) in the culture supernatants. Moreover, superoxide anion was produced by eosinophils after stimulation of the ESP. The ESP-induced EDN release was found to be significantly inhibited when the ESP was pretreated with protease inhibitor cocktail or the cysteine protease inhibitor, E-64. These findings suggest that human eosinophils become degranulated in response to P. westermani-secreted proteases, which may contribute to in vivo tissue inflammation around the worms.
Files in This Item:
T200500459.pdf Download
DOI
10.3347/kjp.2005.43.1.33
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Shin, Myeong Heon(신명헌) ORCID logo https://orcid.org/0000-0001-8207-6110
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/147678
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