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The synthetic chemoattractant peptide WKYMVm induces superoxide production by human eosinophils via the phosphoinositide 3-kinase-mediated activation of ERK1/2
DC Field | Value | Language |
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dc.contributor.author | 신명헌 | - |
dc.contributor.author | 이영아 | - |
dc.date.accessioned | 2017-05-08T08:01:28Z | - |
dc.date.available | 2017-05-08T08:01:28Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 1018-2438 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/147677 | - |
dc.description.abstract | Background: Eosinophils play a key role in allergic inflammation and parasitic infections. The synthetic peptide, Trp-Lys-Tyr-Met-Val-D-Met (WKYMVm), has been previously shown to activate eosinophils and thus to enhance respiratory burst through the formyl peptide receptors. Objective: This study was undertaken to determine the intracellular signaling pathway involved in WKYMVm-stimulated superoxide production by human eosinophils. Methods: Purified eosinophils from peripheral blood were stimulated with various concentrations (10–3 to 10 μM) of WKYMVm and the involvement of PI3-kinase and MAP kinases in WKYMVm-triggered superoxide production was investigated using pharmacological inhibitors. Results: WKYMVm-induced superoxide production by eosinophils was strongly inhibited by pretreatment with the PI3-kinase inhibitor LY294002. In addition, pretreatment with the ERK1/2 kinase inhibitor PD98059 resulted in marked inhibition of superoxide production induced by WKYMVm. Indeed, WKYMVm strongly induced phosphorylation of ERK1/2. The ERK1/2 activation by the peptide was transient and peaked after 2 min of stimulation. Furthermore, ERK1/2 activation by WKYMVm was completely inhibited by pretreatment with the PI3-kinase inhibitor LY294002, but not by the PKC inhibitor Ro-31-8220. Conclusion: These results suggest that WKYMVm stimulates human eosinophils to induce superoxide production via a PI3-kinase-mediated ERK1/2 pathway. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | S. Karger | - |
dc.relation.isPartOf | INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | The synthetic chemoattractant peptide WKYMVm induces superoxide production by human eosinophils via the phosphoinositide 3-kinase-mediated activation of ERK1/2 | - |
dc.type | Article | - |
dc.publisher.location | Switzerland | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Tropica Medicine (열대의학교실) | - |
dc.contributor.department | Dept. of Tropica Medicine (열대의학교실) | - |
dc.contributor.googleauthor | Shin M.H. | - |
dc.contributor.googleauthor | Lee Y.-A. | - |
dc.contributor.googleauthor | Bae Y.-S. | - |
dc.contributor.googleauthor | Kita H. | - |
dc.contributor.googleauthor | Kim Y. | - |
dc.contributor.googleauthor | Ryu S.H. | - |
dc.identifier.doi | 10.1159/000085428 | - |
dc.contributor.localId | A02099 | - |
dc.contributor.localId | A02959 | - |
dc.relation.journalcode | J01074 | - |
dc.identifier.eissn | 1423-0097 | - |
dc.identifier.pmid | 10.1159/000085428 | - |
dc.identifier.url | http://www.karger.com/Article/FullText/85428 | - |
dc.contributor.alternativeName | Shin, Myeong Heon | - |
dc.contributor.alternativeName | Lee, Young Ah | - |
dc.citation.volume | 137 | - |
dc.citation.number | Suppl. 1 | - |
dc.citation.startPage | 21 | - |
dc.citation.endPage | 26 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, Vol.137(Suppl. 1) : 21-26, 2005 | - |
dc.date.modified | 2017-05-04 | - |
dc.identifier.rimsid | 45220 | - |
dc.type.rims | ART | - |
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