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MUC8 as a ciliated cell marker in human nasal epithelium

Authors
 Chang-hoon Kim  ;  Hyun Jik Kim  ;  Kyoung Seob Song  ;  Je-kyung Seong  ;  Kyung-su Kim  ;  Jeung-gweon Lee  ;  Joo-heon Yoon 
Citation
 Acta Oto-Laryngologica, Vol.125(1) : 76-81, 2005 
Journal Title
 Acta Oto-Laryngologica 
ISSN
 0001-6489 
Issue Date
2005
MeSH
Ciliated cell ; MUC8 ; nasal epithelial cells
Keywords
Ciliated cell ; MUC8 ; nasal epithelial cells
Abstract
CONCLUSIONS: This study indicates that MUC8 protein is expressed in ciliated cells from human nasal epithelial cells and is upregulated by IL-1beta treatment. These results suggest that MUC8 gene and protein expression levels could be used as a ciliated cell marker in human nasal epithelium. OBJECTIVES: To examine MUC8 mRNA expression patterns according to the mucociliary differentiation of normal human nasal epithelial (NHNE) cells, and to investigate the localization of MUC8 proteins in nasal polyps. MATERIAL AND METHODS: Passage-2 NHNE cells were cultured using an air-liquid interface technique. On Days 2, 7, 14 and 28 after confluence, ciliated cells were counted by means of cytospin slide immunostaining using H6C5 and beta-tubulin, and MUC8 mRNA levels were determined using real-time quantitative polymerase chain reaction (PCR). After synthesizing polyclonal anti-MUC8 peptide antibodies, MUC8 immunostaining was performed using nasal polyps. MUC8 mRNA and protein levels were determined in NHNE cells treated with IL-1beta (10 ng/ml for 24 h) using reverse transcriptase-PCR and Western blot analysis. RESULTS: The increases in the number of ciliated cells and the MUC8 gene expression level with increasing culture time in the NHNE cells were quite similar. MUC8 was expressed in the ciliated cells of human nasal polyps. The MUC8 protein level and the mRNA level were upregulated as a result of IL-1beta treatment.
Full Text
http://informahealthcare.com/doi/abs/10.1080/00016480410015785
DOI
10.1080/00016480410015785
Appears in Collections:
5. Research Institutes (연구소) > Airway Hucus Institute (기도점액연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Su(김경수) ORCID logo https://orcid.org/0000-0003-1460-0640
Kim, Chang Hoon(김창훈) ORCID logo https://orcid.org/0000-0003-1238-6396
Kim, Hyun Jik(김현직)
Song, Kyoung Seob(송경섭)
Yoon, Joo Heon(윤주헌)
Lee, Jeung Gweon(이정권)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/147530
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