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Loss of Cyclin B1 followed by downregulation of Cyclin A/Cdk2, apoptosis and antiproliferation in Hela cell line

DC FieldValueLanguage
dc.contributor.author김영태-
dc.contributor.author조남훈-
dc.contributor.author최영득-
dc.contributor.author최윤표-
dc.contributor.author홍성희-
dc.contributor.author강숙희-
dc.date.accessioned2017-05-04T07:36:03Z-
dc.date.available2017-05-04T07:36:03Z-
dc.date.issued2005-
dc.identifier.issn0020-7136-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/147526-
dc.description.abstractRecent studies have shown that Cyclin B1 is overexpressed in various tumor types but present at low levels in normal tissues. To explore the possibility of employing Cyclin B1 as an anticancer target, we knocked down Cyclin B1 in an HeLa cell line using RNA interference (RNAi). Subsequently, we monitored cell cycle-related molecules by Western blot together with immunofluorescence and determined cell cycle distribution by flow cytometry. XTT and soft agar colony growth experiments were performed to detect cell viability and proliferation. Furthermore, we analyzed cell apoptosis by measuring Bcl-2 and Bax protein level and DNA-ladder assay. After performing Cyclin B1 RNAi, Cyclin B1, Cyclin A and Cdk2 protein levels were found to be markedly downregulated, whereas Cdc2 was almost unaffected; S-phase fraction increased significantly; HeLa cell viability and cell colony forming ability were markedly diminished after the RNAi; Bcl-2 was noticeably attenuated but Bax was hardly changed; and HeLa cells displayed typical DNA ladder. The loss of Cyclin B1 resulted in the downregulation of Cyclin A and Cdk2, S-phase delay and eventually led to cell apoptosis and the decrease of cell viability and proliferation. Our studies suggest that Cyclin B1 may be a promising anticancer target.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherWiley-Liss-
dc.relation.isPartOfInternational Journal of Cancer-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleLoss of Cyclin B1 followed by downregulation of Cyclin A/Cdk2, apoptosis and antiproliferation in Hela cell line-
dc.typeArticle-
dc.publisher.locationUnited States-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Obstetrics and Gynecology (산부인과학교실)-
dc.contributor.departmentDept. of Pathology (병리학교실)-
dc.contributor.departmentDept. of Urology (비뇨의학교실)-
dc.contributor.departmentDept. of Pathology (병리학교실)-
dc.contributor.departmentDept. of Pathology (병리학교실)-
dc.contributor.googleauthorXian He Xie-
dc.contributor.googleauthorHee Jung An-
dc.contributor.googleauthorSuki Kang-
dc.contributor.googleauthorSunghui Hong-
dc.contributor.googleauthorYoon Pyo Choi-
dc.contributor.googleauthorYoung Tae Kim-
dc.contributor.googleauthorYoung Deuk Choi-
dc.contributor.googleauthorNam Hoon Cho-
dc.identifier.doi10.1002/ijc.21056-
dc.contributor.localIdA00729-
dc.contributor.localIdA03812-
dc.contributor.localIdA04111-
dc.contributor.localIdA04143-
dc.contributor.localIdA04406-
dc.relation.journalcodeJ01092-
dc.identifier.eissn1097-0215-
dc.identifier.urlhttp://onlinelibrary.wiley.com/doi/10.1002/ijc.21056/abstract-
dc.contributor.alternativeNameKim, Young Tae-
dc.contributor.alternativeNameCho, Nam Hoon-
dc.contributor.alternativeNameChoi, Young Deuk-
dc.contributor.alternativeNameChoi, Yoon Pyo-
dc.contributor.alternativeNameHong, Sung Hui-
dc.citation.volume116-
dc.citation.number4-
dc.citation.startPage520-
dc.citation.endPage525-
dc.identifier.bibliographicCitationInternational Journal of Cancer, Vol.116(4) : 520-525, 2005-
dc.date.modified2017-05-04-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Urology (비뇨의학교실) > 1. Journal Papers

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