Cited 13 times in
Proteomic analysis of the proteins expressed by hydrogen peroxide treated cultured human dermal microvascular endothelial cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박윤기 | - |
dc.contributor.author | 방동식 | - |
dc.contributor.author | 이광훈 | - |
dc.contributor.author | 정기양 | - |
dc.date.accessioned | 2017-05-04T07:35:00Z | - |
dc.date.available | 2017-05-04T07:35:00Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 1615-9853 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/147498 | - |
dc.description.abstract | Reactive oxygen species (ROS) have been traditionally regarded as toxic by-products of aerobic metabolism. However, ROS also act as intracellular signaling molecules and can mediate phenotypes in vascular endothelial cells, which may be physiological or pathological in nature. To clarify the molecular mechanisms of ROS signaling, we examined hydrogen peroxide (H(2)O(2))-responsive proteins in cultured human dermal microvascular endothelial cells (HMVEC) using proteomic tools. Protein expression in HMVEC was studied after they had been exposed to low- and high-levels of H(2)O(2) for various times, and intracellular ROS production was examined by flow cytometer and UV spectrophotometer. Proteins obtained from dose- and time-dependent series were separated by two-dimensional gel electrophoresis and tentatively identified by matrix-assisted laser desorption-time of flight mass spectrometry, by matching the tryptic mass maps obtained with entries in the NCBI and Swiss-Prot protein sequence database. At least 163 proteins were changed by H(2)O(2), and 60 proteins were identified. Oxidative stress triggered dramatic change in the expression of proteins in primary microvessel endothelial cells, and their mapping to cellular process provided a view of the ubiquitous cellular changes elicited by H(2)O(2). These results could provide a framework for the understanding of the mechanisms of cellular redox homeostasis and H(2)O(2) metabolism in microendothelium environment in various biological processes as well as pathological conditions. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Wiley-VCH | - |
dc.relation.isPartOf | PROTEOMICS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Amino Acid Sequence | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Cytoplasm/metabolism | - |
dc.subject.MESH | Electrophoresis, Gel, Two-Dimensional | - |
dc.subject.MESH | Endothelial Cells/metabolism* | - |
dc.subject.MESH | Flow Cytometry | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Hydrogen Peroxide/pharmacology* | - |
dc.subject.MESH | Microcirculation/cytology | - |
dc.subject.MESH | Microcirculation/metabolism | - |
dc.subject.MESH | Mitochondria/drug effects | - |
dc.subject.MESH | Mitochondria/metabolism | - |
dc.subject.MESH | Molecular Sequence Data | - |
dc.subject.MESH | Proteome/biosynthesis* | - |
dc.subject.MESH | Reactive Oxygen Species/metabolism | - |
dc.subject.MESH | Skin/blood supply* | - |
dc.subject.MESH | Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization | - |
dc.subject.MESH | Spectrophotometry, Ultraviolet | - |
dc.subject.MESH | beta-Galactosidase/metabolism | - |
dc.title | Proteomic analysis of the proteins expressed by hydrogen peroxide treated cultured human dermal microvascular endothelial cells | - |
dc.type | Article | - |
dc.publisher.location | Germany | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Dermatology (피부과학교실) | - |
dc.contributor.department | Dept. of Dermatology (피부과학교실) | - |
dc.contributor.department | Dept. of Dermatology (피부과학교실) | - |
dc.contributor.department | Dept. of Dermatology (피부과학교실) | - |
dc.contributor.googleauthor | Moon Kyung Ha | - |
dc.contributor.googleauthor | Kee Yang Chung | - |
dc.contributor.googleauthor | Dongsik Bang | - |
dc.contributor.googleauthor | Yoon Kee Park | - |
dc.contributor.googleauthor | Kwang Hoon Lee | - |
dc.identifier.doi | 10.1002/pmic.200401043 | - |
dc.contributor.localId | A01595 | - |
dc.contributor.localId | A01784 | - |
dc.contributor.localId | A02674 | - |
dc.contributor.localId | A03582 | - |
dc.relation.journalcode | J02566 | - |
dc.identifier.eissn | 1615-9861 | - |
dc.identifier.pmid | 15838903 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1002/pmic.200401043/abstract | - |
dc.subject.keyword | 15838903 | - |
dc.contributor.alternativeName | Park, Yoon Kee | - |
dc.contributor.alternativeName | Bang, Dong Sik | - |
dc.contributor.alternativeName | Lee, Kwang Hoon | - |
dc.contributor.alternativeName | Chung, Kee Yang | - |
dc.citation.volume | 5 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 1507 | - |
dc.citation.endPage | 1519 | - |
dc.identifier.bibliographicCitation | PROTEOMICS, Vol.5(6) : 1507-1519, 2005 | - |
dc.date.modified | 2017-05-04 | - |
dc.identifier.rimsid | 40277 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.