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간세포성장인자가 하인두 편평세포암 세포주에서 Matrix Metalloproteinase(MMP)-2, 9과 Urokinase Type Plasmogen Activator에 미치는 영향
DC Field | Value | Language |
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dc.contributor.author | 최은창 | - |
dc.date.accessioned | 2017-05-04T07:26:16Z | - |
dc.date.available | 2017-05-04T07:26:16Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 1225-035X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/147287 | - |
dc.description.abstract | Background and Objectives:Recent reports revealed that hepatocyte growth factor (HGF) is related to tumor invasion and metastasis. Activation of the urokinase-type plasminogen activator (u-PA)/plasmin proteolytic network and matrix metalloproteinase has been shown to play a key role in tumor invasion and dissemination of various malignancies. So we examined the effect of HGF/c-Met on MMP-2, 9 and u-PA in FaDu cell, a hypopharyngeal squamous cell carcinoma cell line. Materials and Method:We performed RT-PCR and Western blot in FaDu. Tumor cell invasiveness was assessed by the membrane invasion assay (using Transwell chamber). To examine the role of MMP-2, 9 and the relation between HGF and MMP in the invasion of hypopharyngeal cancer, RT-PCR and zymography were performed in FaDu cells. We tested to confirm the HGF-mediated plasmin activation. Results:The expressions of c-Met mRNA and protein were detected in the hypopharyngeal cell line while that of HGF was not. HGF markedly enhanced the invasion of cancer cells in a Transwell invasion chamber in a dose-dependent manner (p<0.05). The expression of MMP was detected in hypopharyngeal cancer cells and exogenous HGF slightly enhanced the induction of MMP-2 activity in zymogram analysis. The activity of u-PA was detected in FaDu and HGF (above 10 units/mL) enhanced the activity of u-PA (p<0.05). Conclusion:These results suggest that HGF may play an important role in hypopharyngeal cancer through the activation of u-PA and matrix metalloproteinase. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 788~795 | - |
dc.language | Korean | - |
dc.publisher | 대한이비인후과학회 | - |
dc.relation.isPartOf | Korean Journal of Otolaryngology (대한이비인후과학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Hepatocyte growth factor | - |
dc.subject.MESH | c-Met, Hypopharyngeal cancer | - |
dc.subject.MESH | Invasion | - |
dc.subject.MESH | Urokinase-type plasminogen activator | - |
dc.subject.MESH | Matrix metalloproteinase | - |
dc.title | 간세포성장인자가 하인두 편평세포암 세포주에서 Matrix Metalloproteinase(MMP)-2, 9과 Urokinase Type Plasmogen Activator에 미치는 영향 | - |
dc.title.alternative | Effect of HGF in Invasion of Hypopharyngeal Squamous Cell Carcinoma Cell Line | - |
dc.type | Article | - |
dc.publisher.location | Korea | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Otorhinolaryngology (이비인후과학교실) | - |
dc.contributor.googleauthor | 김철호 | - |
dc.contributor.googleauthor | 문성균 | - |
dc.contributor.googleauthor | 배준호 | - |
dc.contributor.googleauthor | 이재호 | - |
dc.contributor.googleauthor | 최은창 | - |
dc.identifier.doi | OAK-2005-02362 | - |
dc.contributor.localId | A04161 | - |
dc.relation.journalcode | J02088 | - |
dc.relation.journalsince | 1958~2009 | - |
dc.relation.journalafter | 2009~ Korean Journal of Otorhinolaryngology-Head and Neck Surgery | - |
dc.subject.keyword | Hepatocyte growth factor | - |
dc.subject.keyword | c-Met, Hypopharyngeal cancer | - |
dc.subject.keyword | Invasion | - |
dc.subject.keyword | Urokinase-type plasminogen activator | - |
dc.subject.keyword | Matrix metalloproteinase | - |
dc.contributor.alternativeName | Choi, Eun Chang | - |
dc.citation.volume | 48 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 788 | - |
dc.citation.endPage | 795 | - |
dc.identifier.bibliographicCitation | Korean Journal of Otolaryngology (대한이비인후과학회지), Vol.48(6) : 788-795, 2005 | - |
dc.date.modified | 2017-05-04 | - |
dc.identifier.rimsid | 48460 | - |
dc.type.rims | ART | - |
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